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Elisa max mouse ifn γ kit

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The ELISA MAX mouse IFN-γ kit is a laboratory assay used to quantify the levels of interferon-gamma (IFN-γ) in mouse samples. It provides a standardized and reliable method to measure this cytokine.

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Mouse IFN-γ ELISA MAX Mouse IFN-γ ELISA IFN-γ ELISA kit IFN-γ ELISA Mouse IFN-γ ELISA MAX kits IFN-γ Mouse ELISA kits ELISAs

2 protocols using elisa max mouse ifn γ kit

1

Splenocyte IFN-γ Production Assay

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Splenocytes were prepared from the various groups of mice, plated in a concentration of 1 × 106 cells/well and RgpA (1 µg/ml) was added for 3 days. Supernatants were then collected and the level of IFN-γ in the supernatants was measured using an ELISA MAX mouse IFN-γ kit (BioLegend) according to the manufacturer’s instructions. Cytokine levels were determined using standard curves of recombinant cytokines and are expressed as picogram per milliliter.
Nassar M., Tabib Y., Capucha T., Mizraji G., Nir T., Saba F., Salameh R., Eli-Berchoer L., Wilensky A., Burstyn-Cohen T, & Hovav A.H. (2018). Multiple Regulatory Levels of Growth Arrest-Specific 6 in Mucosal Immunity Against an Oral Pathogen. Frontiers in Immunology, 9, 1374.
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2

Measuring Mouse IFN-γ by ELISA

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The level of IFN-γ in the supernatants of the ex vivo antigen presentation assay and the splenocyte re-stimulation assay was measured using an ELISA MAX mouse IFN-γ kit (BioLegend) according to the manufacturer’s instructions. Cytokine levels were determined using standard curves of recombinant cytokines and are expressed as picogram per milliliter.
Mizraji G., Heyman O., Van Dyke T.E, & Wilensky A. (2018). Resolvin D2 Restrains Th1 Immunity and Prevents Alveolar Bone Loss in Murine Periodontitis. Frontiers in Immunology, 9, 785.
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