Hct mass spectrometer

Manufactured by Bruker

Sourced in Germany

The HCT mass spectrometer is a high-performance analytical instrument designed for the detection and identification of chemical compounds. It utilizes ion trap technology to provide accurate mass measurements and sensitive detection of a wide range of analytes. The HCT mass spectrometer is a versatile tool for applications in various fields, including chemical analysis, environmental monitoring, and life sciences.

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Lab products found in correlation

Inlab expert pro ism ph probe, by Mettler Toledo (2 mentions) Spectrum two spectrometer, by PerkinElmer (2 mentions) Lc290, by Berthold Technologies (1 mentions) Lc 200, by PerkinElmer (1 mentions) Discovery bio wide pore c18, by Merck Group (1 mentions) Uv vis spectrophotometer, by Jasco (1 mentions) Dualflex, by Synergy Software (1 mentions) Ac 400 mhz, by Bruker (1 mentions) Autoflex 2, by Bruker (1 mentions) Uv 2550 uv vis spectrophotometer, by Shimadzu (1 mentions) Ac 400 and 600 mhz nmr spectrometer, by Bruker (1 mentions) Ls 55 fluorescence spectrometer, by PerkinElmer (1 mentions) Uv 2600 spectrophotometer, by Shimadzu (1 mentions)

Close spelling variants of this product

HCT Ultra ETDII Ion-trap Mass Spectrometer Esquire HCT Plus Mass Spectrometer HCT Ultra Ion Trap mass spectrometer HCT ion trap mass spectrometer Esquire HCT mass spectrometer HCT Ultra 3D-Ion Trap mass spectrometer Esquire HCT ion trap mass spectrometer Bruker spectrometers

4 protocols using hct mass spectrometer

HPLC Analysis of Radioactive Compounds

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HPLC analysis of the described compounds (cold and radioactive) was performed using the same chromatographic equipment, a Perkin-Elmer LC 200 analytical HPLC coupled to an LC 290 UV/Vis detector and a Berthold LB-507 A radiometric detector. The column used was a Supelco Discovery BIO WidePore C18 (250 × 4.6 mm, 5 μm particle size). Mobile Phases A: H₂O (TFA 0.1%) B: ACN (TFA 0.1%). Gradient program: 5 min A-95%, 20 min going from A-95% to B-100%, 1 min going back to A-95% and 4 min A-95%. Mass spectra were acquired in an electrospray ionization/quadrupole ion trap (ESI/QITMS) Bruker HCT mass spectrometer. Samples were injected in mixtures of H₂O:ACN at a flow rate of 150 µL h⁻¹.

D’Onofrio A., Silva F., Gano L., Raposinho P., Fernandes C., Sikora A., Wyczółkowska M., Mikołajczak R., Garnuszek P, & Paulo A. (2022). Bioorthogonal Chemistry Approach for the Theranostics of GRPR-Expressing Cancers. Pharmaceutics, 14(12), 2569.

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Synthesis and Characterization of Novel Nucleoside Analogue

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All chemical reagents and solvents were obtained from Sigma-Aldrich (Ireland) Ltd. and unless otherwise stated were used without further purification. C8-Alkyne-dU-CEP was purchased from BaseClick GmbH. NMR spectra were recorded on Bruker AC 400 MHz or 600 MHz spectrometers (Supplementary S1). FT-IR spectra were collected on Perkin Elmer Spectrum Two spectrometer. ESI-MS analysis was performed on a Bruker HCT Mass Spectrometer. pH was monitored using a Mettler Toledo InLab Expert Pro-ISM pH probe. Crystallographic data was collected at 100(1)K on a Synergy Dualflex, AtlasS2 diffractometer (Supplementary S2). The structure was solved by dual space methods and refined on F2 using all the reflections (SHELXL-2018). Mass analysis of oligonucleotides was characterised at Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) on a Bruker Daltonics Autoflex II instrument (Supplementary S3). Thermal melting experiments were conducted on Agilent Cary 100 UV-Vis dual beam spectrophotometer equipped with a 6 × 6 Peltier multicell system with temperature controller. DNA was quantified on a Jasco UV-Vis spectrophotometer. Polyacrylamide gels were imaged on Syngene G:Box mini 9 gel documentation system.

Lauria T., Slator C., McKee V., Müller M., Stazzoni S., Crisp A.L., Carell T, & Kellett A. (2020). A Click Chemistry Approach to Developing Molecularly Targeted DNA Scissors. Chemistry (Weinheim an der Bergstrasse, Germany), 26(70).

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Comprehensive Analytical Characterization

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Mass spectrometry (MS) was performed on a Bruker HCT mass spectrometer (Bruker Co., Karlsruhe, Germany), interfaced with an atmospheric pressure chemical ionization ion source (i.e., APCI-MS) in negative ion mode. Single-crystal X-ray diffraction data were collected on an Agilent SuperNova diffractometer (Agilent Technologies Ltd., Cheadle, UK) using a Cu Kα (λ = 1.54184 Å) microfocus X-ray source. The crystal data processing was carried out through CrysAlis^Pro3. Within Olex2 [30 (link)], the structures were solved with the SHELXT and SHELXL-2015 [31 (link)] programs (George M. Sheldrick, Georg-August Universität Göttingen, Tammannstraße 4, Göttingen 37077, Germany), using the intrinsic phasing method, and refined using the full-matrix least-squares analysis based on F². The SQUEEZE program, part of the crystallographic software PLATON package (Version: 91117, (C) 1980–2021 A.L.Spek, Utrecht University, Utrecht, The Netherlands) [32 (link)], was used to calculate the disordered area of solvent and remove its contributions from the intensity data, as needed. The UV-Vis spectra were recorded on a Shimadzu UV-2550 UV-Vis spectrophotometer (Shimadzu Co., Kyoto, Japan), with redistilled toluene as a solvent.

Xie F.F., Chen Z.C., Wu Y.H., Tian H.R., Deng S.L., Xie S.Y, & Zheng L.S. (2022). Carbene Addition Isomers of C70 formed in the Flame of Low-Pressure Combustion. Nanomaterials, 12(18), 3087.

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Characterization of Chemical Compounds

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Chemicals, reagents and high-performance liquid chromatography (HPLC) grade solvents including CHCl3, MeOH and CH3CN were sourced from Sigma-Aldrich (Ireland) or Tokyo Chemical Industry (TCI, UK Ltd) and used without further purification. 1 H and 13 C NMR spectra were obtained on a Bruker AC 400 and 600 MHz NMR spectrometer. pH was monitored by a Mettler Toledo InLab Expert Pro-ISM pH probe. Electrospray ionization mass spectrometry (ESI-MS) measurements were recorded using a ion trap Bruker HCT mass spectrometer with samples being prepared in 100% HPLC-grade CH3CN prior to ESI-MS analysis. UV/Vis absorption spectroscopy studies were carried out on a Shimadzu UV-2600 spectrophotometer. FTIR measurements were conducted on a PerkinElmer Spectrum Two spectrometer. Fluorescence DNA binding studies were carried out on a Perkin Elmer LS55 Fluorescence Spectrometer.

Fantoni N.Z., Molphy Z., O'Carroll S., Menounou G., Mitrikas G., Krokidis M.G., Chatgilialoglu C., Colleran J., Banasiak A., Clynes M., Roche S., Roche S., Kelly S., McKee V, & Kellett A. (2021). Polypyridyl-Based Copper Phenanthrene Complexes: Combining Stability with Enhanced DNA Recognition. Chemistry (Weinheim an der Bergstrasse, Germany), 27(3).

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