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Cannabidiol cbd

Manufactured by Cayman Chemical
Sourced in United States

Cannabidiol (CBD) is a naturally occurring compound found in the cannabis plant. It is a chemical that can be extracted and purified for use in various applications. CBD has a range of potential uses, but its core function is to serve as a research compound for scientific study.

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3 protocols using cannabidiol cbd

1

CBD Administration for Seizure Induction

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Cannabidiol (CBD, Cayman Chemical) was dissolved in a 1:1:18 ratio of 100% ethanol, cremophore, and 0.9% saline, respectively as previously described (Klein et al., 2017 (link)). CBD or vehicle was administered (intraperitoneal, i. p.) 2 hours prior to seizure induction or behavioral assessments. Pentylenetetrazole (PTZ, Sigma-Aldrich) was dissolved in 0.9% saline.
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2

Cannabidiol and THC Extraction and Verification

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Cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) were purchased from Cayman Chemical (Ann Arbor, MI, USA). CBD extract was produced through supercritical CO2 extraction from hemp (Helping Hands Hemp, Womelsdorf, PA, cultivar: YoungSim 10) and THC extract was provided by the NIDA drug program (Research Triangle Institute, Research Triangle Park, NC, USA) the composition of both was verified by an independent laboratory (Keystone State Testing, Harrisburg, PA, USA) Cisplatin was purchased from Acros Organics (Fairlawn, NJ, USA).
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3

Cannabinoid Standards Preparation

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Cannabidiol (CBD), Δ9-tetrahydrocannabinol (THC), CBD-d9,
and THC-d3 chemical standards (1 mg/mL)
and solid CBD were purchased from Cayman Chemical (Ann Arbor, MI).
Methanol was obtained from Pharmco (Brookfield, CT). Polyethylene
glycol (PEG 600) was purchased from Sigma-Aldrich (St. Louis, MO).
Nitrogen and ultra-high-purity helium gases were acquired from Airgas
(Albany, NY).
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