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Wright giemsa stain kit

Manufactured by Abcam

The Wright-Giemsa stain kit is a laboratory reagent used for the differential staining of blood cells. The kit contains the necessary components to prepare and stain blood smears, allowing for the identification and classification of various blood cell types under a microscope.

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3 protocols using wright giemsa stain kit

1

Virus-Mediated Cell Membrane Fusion Assay

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Virus-mediated cell membrane FFWI was performed as previously described (Guirakhoo et al., 1993 (link)). Essentially, C6/36 cells were seeded onto glass chamber slides (ThermoFisher, Waltham, MA) before infection with VEEV TC83 at an MOI of 1.0 and incubation at 28 °C/5%CO2 while maintaining the pH at 7.5. Twenty-four hours after infection, cells were incubated with various concentrations of MAb diluted in BA-1 for 2 h at 28 °C/5%CO2. After MAb treatment, cells were exposed to fusion medium (DMEM buffered at pH < 5.0 with 2-N-morpholinoethanesulfonic acid) for 1 h, after which fusion medium was replaced with DMEM at neutral pH. Cells were incubated another 24 h at 28 °C/5%CO2 before being fixed in absolute methanol and stained using the Wright-Giemsa stain kit (Abcam, Cambridge, MA). An isotype MAb (100 μg/ml), virus-infected non-treated cells at pH 7.5, and uninfected MAb-treated cells at pH 5.0 were included as controls. The number of nuclei and the number of cells in five microscopic fields (magnification 100-fold) were counted and the fusion index [1 - (number of cells/number of nuclei)] was calculated. A one-way ANOVA with Tukey’s multiple comparisons test was used to compare differences in percent fusion.
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2

BALF Sample Preparation and Staining

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Each BALF sample was centrifuged at 300 × g for 10 min in a cytocentrifuge with a slide for attaching the cells. Then, the slide was stained using a Wright-Giemsa Stain Kit (Abcam, Dawinbio Inc., Hanam, Gyeonggi-do, Republic of Korea, ab245888), according to the manufacturer’s instructions.
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3

Virus-Mediated Cell Membrane Fusion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus-mediated cell membrane FFWI was performed as previously described (Guirakhoo et al., 1993 (link)). Essentially, C6/36 cells were seeded onto glass chamber slides (ThermoFisher, Waltham, MA) before infection with VEEV TC83 at an MOI of 1.0 and incubation at 28 °C/5%CO2 while maintaining the pH at 7.5. Twenty-four hours after infection, cells were incubated with various concentrations of MAb diluted in BA-1 for 2 h at 28 °C/5%CO2. After MAb treatment, cells were exposed to fusion medium (DMEM buffered at pH < 5.0 with 2-N-morpholinoethanesulfonic acid) for 1 h, after which fusion medium was replaced with DMEM at neutral pH. Cells were incubated another 24 h at 28 °C/5%CO2 before being fixed in absolute methanol and stained using the Wright-Giemsa stain kit (Abcam, Cambridge, MA). An isotype MAb (100 μg/ml), virus-infected non-treated cells at pH 7.5, and uninfected MAb-treated cells at pH 5.0 were included as controls. The number of nuclei and the number of cells in five microscopic fields (magnification 100-fold) were counted and the fusion index [1 - (number of cells/number of nuclei)] was calculated. A one-way ANOVA with Tukey’s multiple comparisons test was used to compare differences in percent fusion.
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