G4900da surescan microarray scanner

The WGA was performed using REPLI-g Single Cell Kit (Qiagen, Hilden, Germany) and following the manufacturer’s instructions. Amplified DNA was purified using the QIAamp DNA Blood Mini Kit (Qiagen). The DNA purities and concentrations were examined by Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Delaware, USA). Approximately 1 μg of purified DNA was fluorescently labeled with Cy3 d-CTP or Cy5-dCTP using SureTag DNA Labeling Kit (Agilent Technologies, CA, USA) and then cleaned up by Microcon YM-30 centrifugal filter unit (Millipore, MA, USA). The yield DNA was hybridized with CytoScan 60 K microarray chip (Agilent customer array, Changhua Christian Hospital, Taiwan) at 65 °C for 2 h. The image on a chip was acquired with a G4900DA SureScan microarray scanner (Agilent Technologies, CA, USA) and analyzed with Agilent Genomic Workbench software (Agilent Technologies) for DNA gain or loss across all 24 chromosomes. Aberrations were detected by using default setting with an algorithm of z-score conjugated with a filter of a minimum of 5 Mb aberrations. All aCGH procedures were completed within 9 h, allowing rapid evaluation of the TE biopsies and providing a feasible opportunity for fresh embryo transfer on the following day, Day 6.

Approximately 1 μg of purified WGA DNA was fluorescently labeled with Cy3 d-CTP or Cy5-dCTP using the SureTag DNA Labeling Kit (Agilent Technologies, CA, USA) and then cleaned up by a Microcon YM-30 centrifugal filter unit (Millipore, MA, USA). The yield DNA was hybridized with a CytoScan 60 × 8K microarray chip (Agilent customer array, Changhua Christian Hospital, Changhua, Taiwan) following the manufacturer’s instructions. The image on a chip was acquired with a G4900DA SureScan microarray scanner (Agilent Technologies) and analyzed with Agilent Genomic Workbench software (Agilent Technologies) for chromosome gain or loss across all 24 chromosomes. Aberrations were detected using the default setting with the z-score algorithm conjugated with a filter of a minimum of 5 Mb aberrations.