Quantstudio 12k flex openarray accufill system

Subjects had DNA isolated from blood obtained via standard venipuncture at ACNW in Springdale, Arkansas, USA. Samples were either sent to OneOme, LLC (Minneapolis, MN, USA) for the RightMed^® test (https://oneome.com/rightmed-test/; assessed 20 January 2022) or Arkansas Children’s Hospital (ACH) Pathology for the PGx test [12 (link)]. The patient ID, date of birth, sex, ethnic background, and medications list were submitted to the OneOme portal or the ACH portal (Figure 1). OneOme analyzes patient DNA on an IntelliQube qPCR platform (Douglas Scientific, Alexandria, MN, USA), which employs 111 TaqMan single-nucleotide polymorphisms (SNPs) genotyping and copy number variation assays for 27 genes (https://oneome.com/rightmed-test/; assessed 20 January 2022). ACH-PGx uses an OpenArray^® (ThermoFisher Scientific, Carlsbad, CA, USA) plate using the QuantStudio™12K Flex OpenArray AccuFill System and assays 174 TaqMan^® SNPs (ThermoFisher Scientific, Carlsbad, CA, USA) targeting 23 genes [12 (link)]. The OneOme and ACH-PGx laboratories are accredited by the College of American Pathologists and certified by the Clinical Laboratory Improvement Amendments (CLIA).