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Liquichip m100 reader

Manufactured by Qiagen
Sourced in United States

The Liquichip M100 reader is a compact and versatile fluorescence detection system designed for quantitative analysis of assays on microarray chips. It provides accurate and reliable data acquisition and analysis for a wide range of applications, including gene expression profiling, protein detection, and diagnostic testing.

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2 protocols using liquichip m100 reader

1

Multiplex assay for maternal, cord IgG

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The multiplex assay was performed as previously described41 (link). Briefly, 50 μl of maternal or cord plasma (diluted 1:500) was mixed with 50 µl pooled microspheres (3000 microspheres/antigen), incubated 1 h, washed, and then incubated with 100 µl of 2 µg/ml of R-phycoerythrin-conjugated, F(ab')2 fragment, goat anti-human IgG (Fcγ fragment specific) (Jackson ImmunoResearch). The microspheres were washed and read in a Liquichip M100 reader (QIAGEN). Results were expressed as median fluorescence intensity (MFI). The positive control consisted of 36 plasma samples pooled from multigravidae used at 3 dilutions. Negative controls was pooled plasma from 90 individuals in the USA not exposed to malaria.
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2

IgG Subclass Quantification Assay

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For IgG subclass assay, each plasma sample was tested in quadruplicates. After initial incubation of FV2-coupled microspheres with plasma and washing as described above, microspheres were incubated with 100 µL mouse anti-human Ab to detect specific IgG subclasses: (a) mouse anti-human IgG1 (Molecular Probes, Ref. A10630) 1:1000 dilution, (b) mouse anti-human IgG2 (Sigma, ascites HP6014 I5635) 1:1000 dilution, (c) mouse anti-human IgG3 (Sigma, clone HP6050 I7260) 1:2000 dilution, and, (d) mouse anti-human IgG4 (Sigma HP6023 I9888) 1:1000 dilution. Plates were incubated for 1 h, at 25 °C on the shaker. After washing as described above, microspheres were resuspended in 100 µl of R-phycoerythrin-conjugated donkey anti-mouse IgG H + L (Jackson 715-116-150) at 1:250 dilution. Plates were incubated for 1 h, at 25 °C on a shaker, washed as before and resuspended in 100 µl PBS-1 % BSA; 85 µl of the microsphere suspension was analysed using a Liquichip M100 reader (Qiagen, Valencia, CA, USA). To test the quality of mouse-anti-human Ab, beads coupled to human monoclonal IgG1k, human monoclonal IgG2k, human monoclonal IgG3λ and human monoclonal IgG4λ were used. These beads were assayed as described above. Each sub-class was highly specific with no significant cross-reactivity with other IgG sub-classes (Additional file 2).
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