Bovine serum albumin (BSA), rabbit serum albumin (RSA), ovalbumin (OVA), 3,4‐dichloroaniline, atrazine desethyl (6‐Chloro‐N,N‐dimethyl‐1,3,5‐triazine‐2,4‐diamine), N‐(1‐naphthyl)‐ethylene‐diamine, 2‐benzimidazole propionic acid, aflatoxin‐BSA conjugate (hapten density of 8‐12), N‐hydroxysuccinimide (NHS) ester, O‐phenylenediamine dihydrochloride (OPD), 2‐(N‐morpholino) ethanesulfonic acid (MES), and dimethylformamide were purchased from Sigma‐Aldrich (St. Louis, MO). Goat anti‐human IgG+IgM+IgA‐HRP labeled Abs were purchased from Abcam (Cambridge, MA). Vacutainer tubes were obtained from BD Diagnostics (Franklin Lakes, NJ). EDC [1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide] and Nunc maxiSorp flat‐bottom plates were purchased from Thermo Fisher Scientific (Waltham, MA). All the reagents and solvents were of analytical grade.
N hydroxysuccinimide nhs ester
Manufactured by Merck Group
Sourced in Sao Tome and Principe
N‐hydroxysuccinimide (NHS) ester is a type of lab equipment used in various chemical reactions. It is a reactive compound that can be used to attach other molecules to target compounds. NHS esters are commonly used in protein modification, bioconjugation, and other applications where the formation of amide bonds is required.
Automatically generated - may contain errors
Lab products found in correlation
2 n morpholino ethanesulfonic acid mes, by Merck Group (1 mentions)
3 4 dichloroaniline, by Merck Group (1 mentions)
Nunc maxisorp flat bottom plate, by Thermo Fisher Scientific (1 mentions)
O phenylenediamine dihydrochloride opd, by Merck Group (1 mentions)
N 1 naphthyl ethylenediamine, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
Vacutainer tube, by BD (1 mentions)
Dbco peg4 nhs ester, by Vector Laboratories (1 mentions)
Mtt reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using n hydroxysuccinimide nhs ester
1
Hapten-Based Competitive ELISA Protocol
2
Liposome-Mediated Protein Conjugation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All of the solvents and reagents
were purchased from commercial sources and used as received, unless
otherwise noted. Deionized water was used as a solvent in all of the
procedures. N-Hydroxysuccinimide (NHS-ester) and
cholesteryl chloroformate were purchased from Sigma-Aldrich (St. Louis,
MO). 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine,
1,2-distearoyl-sn-glycero-3-phosphoethanolamine–N-[amino (poly(ethylene glycol))-2000] (ammonium salt) (DSPE–PEG2000-NH2) were purchased from Avanti Polar Lipids,
Inc (Alabaster, AL). DBCO–PEG4-NHS ester was purchased
from Click Chemistry tools (Scottsdale, AZ). Azido–PEG4–biotin and streptavidin-fluorescein isothiocyanate
(streptavidin-FITC) were purchased from Biolegend (San Diego, CA).
Dulbecco’s modified Eagle’s medium (DMEM), (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide), and MTT reagent were purchased from Life Technologies (Grand
Island, NY).
were purchased from commercial sources and used as received, unless
otherwise noted. Deionized water was used as a solvent in all of the
procedures. N-Hydroxysuccinimide (NHS-ester) and
cholesteryl chloroformate were purchased from Sigma-Aldrich (St. Louis,
MO). 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine,
1,2-distearoyl-sn-glycero-3-phosphoethanolamine–N-[amino (poly(ethylene glycol))-2000] (ammonium salt) (DSPE–PEG2000-NH2) were purchased from Avanti Polar Lipids,
Inc (Alabaster, AL). DBCO–PEG4-NHS ester was purchased
from Click Chemistry tools (Scottsdale, AZ). Azido–PEG4–biotin and streptavidin-fluorescein isothiocyanate
(streptavidin-FITC) were purchased from Biolegend (San Diego, CA).
Dulbecco’s modified Eagle’s medium (DMEM), (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide), and MTT reagent were purchased from Life Technologies (Grand
Island, NY).
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