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Alexa fluor 488 anti goat antibody

Manufactured by Thermo Fisher Scientific

Alexa Fluor 488-anti-goat antibody is a fluorescently labeled secondary antibody used for the detection and visualization of goat primary antibodies in various immunoassays and imaging applications. The Alexa Fluor 488 dye provides bright, photostable fluorescence at the green wavelength range.

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2 protocols using alexa fluor 488 anti goat antibody

1

Apoptosis Detection via TUNEL Assay

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Transferase dUTP nick end labeling (TUNEL) assay (In Situ Cell Death Detection Kit; Roche) was performed as instructed. The samples were stained with goat-anti-DsRed (Abcam; 1:400) and rabbit-anti-Lcp1 antibody (1:200), followed by incubation with Alexa Fluor 488-anti-goat antibody (Invitrogen; 1:400) and Alexa Fluor 488-anti-rabbit antibody (Invitrogen; 1:400) for fluorescent visualization.
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2

BrdU labeling of Tg(hsp70:E2A-PBX1-EGFP) embryos

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Sibling and Tg(hsp70:E2A-PBX1-EGFP) embryos at 5-6 days post-fertilization (dpf) were incubated in 10 mM bromodeoxyuridine (BrdU) (Sigma-Aldrich) for 4 hours. The embryos were then stained with goat-anti-DsRed (Abcam; 1:400) and rabbit-anti-Lcp1 monoclonal antibody (1:200), followed by incubation with Alexa Fluor 488-anti-goat antibody (Invitrogen; 1:400) and Alexa Fluor 488-anti-rabbit antibody (Invitrogen; 1:400) for fluorescent visualization, according to the provided instructions.
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