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2 protocols using osimertinib

1

Osimertinib NSCLC Immunohistochemistry Protocol

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Osimertinib was purchased from Toronto Research Chemicals (Toronto, Canada). Paraformaldehyde and hydrogen peroxide (30% in water) were acquired from Nacalai Tesque (Tokyo, Japan). Histofine Simple Stain MAX-PO (M) was purchased from Nichirei Bioscience (Tokyo, Japan). 3,3,5,5-Tetramethylbenzidine (TMB) was purchased from Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). 3,3'-Diaminobenzidine tetrahydrochloride (DAB) was purchased from Junsei Chemical (Tokyo, Japan). Bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 1-Methylindole and N,N'-dimethylethylenediamine were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The other reagents and solvents were of the highest grades commercially available.
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2

Quantitative Analysis of EGFR-TKI Drugs

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The analytes including gefitinib (lot: F1102AS), icotinib (lot: J0615A), afatinib (lot: M0320A), erlotinib (lot: J0615A), crizotinib (lot: A0320A), and carbamazepine (internal standard, IS) (lot: M1001AS) were supplied by Meilun Biotech Co., Ltd. (Dalian City, China). Osimertinib (lot: 1-NJL-79-1) was purchased from Toronto Research Chemicals (Toronto, Canada). Mass spectrometric reagents methanol and acetonitrile were obtained from Merck (Merck Company, Darmstadt, Germany).
Formic acid was purchased from Tedia Company Inc. (Tedia, Fairfield, OH, USA). Isopropanol reagent was purchased from Shanghai Titan Technology Co., Ltd. (Titan, Shanghai, China). Distilled water was purchased from Watsons Distilled Water Co., Ltd. (Watsons, Guangzhou, China). Human blank plasma was donated by healthy volunteers in our laboratory (Shanghai, China).
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