Total proteins were extracted from cells treated with ZNC97018978 using RIPA lysis buffer (Solarbio, R0010), and the supernatant collected from centrifugation (13,000 rpm, 10 min) was analyzed by BCA assay kit to examine protein concentration. Electrophoresis (10% separation gel and 5% spacer gel) was performed to separate proteins, which were then transferred to a biofilm. Antigenantibody reactions were achieved by addition of primary antibodies overnight at 4 ℃. Hybridization was obtained with secondary antibodies for 1.5 h at 37 ℃. ECL method was used to visualize the protein bands. Image J was applied to perform quantitative analysis. Primary antibodies included AURKA (GeneTex, GTX13824), Caspase-3 (GeneTex, GTX110543), Bax (Abcam, ab32503), Bcl-2 (Abcam, ab196495), CDK1 (Abcam, ab18) and β-actin (Immunoway, YM3028).
Aurka
Manufactured by GeneTex
AURKA is a protein kinase that plays a crucial role in the regulation of cell division and centrosome function. It is involved in the control of mitotic spindle assembly and chromosome segregation during cell division.
Automatically generated - may contain errors
2 protocols using aurka
1
Protein Expression Analysis of ZNC97018978
2
Protein Expression Analysis of ZNC97018978
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Total proteins were extracted from cells treated with ZNC97018978 using RIPA lysis buffer (Solarbio, R0010), and the supernatant collected from centrifugation (13,000 rpm, 10 min) was analyzed by BCA assay kit to examine protein concentration. Electrophoresis (10% separation gel and 5% spacer gel) was performed to separate proteins, which were then transferred to a biofilm. Antigenantibody reactions were achieved by addition of primary antibodies overnight at 4 ℃. Hybridization was obtained with secondary antibodies for 1.5 h at 37 ℃. ECL method was used to visualize the protein bands. Image J was applied to perform quantitative analysis. Primary antibodies included AURKA (GeneTex, GTX13824), Caspase-3 (GeneTex, GTX110543), Bax (Abcam, ab32503), Bcl-2 (Abcam, ab196495), CDK1 (Abcam, ab18) and β-actin (Immunoway, YM3028).
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