The analytical standards employed for the determinations were purchased from Sigma-Aldrich (St. Louis, MO, USA) for o-Tyr, Phe, 3NO2-Tyr, 3Cl-Tyr, p-Tyr, 8OHdG and 2dG (>96% w/w purity). The isotopically labeled compounds used as internal standards were deuterated phenylalanine (Phe-D5) from CDN Isotopes (Pointe-Claire, QC, Canada); 8-Oxo-2′-deoxyguanosine-13C15N2 (8OHdG-13C15N2) and 2′-deoxyguanosine-13C15N2 (2dG-13C15N2) from Toronto Research Chemicals (Toronto, ON, Canada); and deuterated para-tyrosine (p-Tyr-D2) from Cambridge Isotope Laboratories (Tewksbury, MA, USA). The purity of all isotopic labeled compounds was > 98% w/w. Milli-Q® grade water (>18.2 MΩ) was used from a Milli-Q® Integral system (Darmstadt, Germany). Solvents used (Methanol (CH3OH) and acetonitrile (CH3CN)) (LC-MS grade) were obtained from JT Baker (Deventer, The Netherlands) and sodium hydroxide, phosphoric acid, and formic acid (HCOOH) were purchased from Panreac Química (Barcelona, Spain).
Acetonitrile ch3cn
Manufactured by Avantor
Sourced in United States
Acetonitrile (CH3CN) is a colorless, volatile, and flammable organic solvent. It is a polar aprotic solvent commonly used in various analytical and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol ch3oh, by Avantor (2 mentions)
3no2 tyr, by Merck Group (1 mentions)
Deuterated phenylalanine phe d5, by CDN Isotopes (1 mentions)
Lc ms grade, by Avantor (1 mentions)
Ethyl acetate, by ITW Reagents (1 mentions)
Pgf2a d4, by Cayman Chemical (1 mentions)
15 keto 15 e 2t isop, by Cayman Chemical (1 mentions)
15 keto 15 f 2t isop, by Cayman Chemical (1 mentions)
Potassium hydroxide koh, by Merck Group (1 mentions)
Heptane, by Avantor (1 mentions)
Milli q system, by Merck Group (1 mentions)
2 protocols using acetonitrile ch3cn
1
Quantifying Oxidative Stress Biomarkers
2
Quantifying Eicosanoids and Docosanoids
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Commercially available IsoPs (2,3-dinor-15-F 2t -IsoP, 15-keto-15-E 2t -IsoP, 15-keto-15-F 2t -IsoP, 15-epi-15-F 2t -IsoP, 15-E 2t -IsoP, 15-F 2t -IsoP), prostaglandins (PGs) (PGE 2 , PGF 2a , 2,3-dinor-11b-PGF 2a , 11b-PGF2a, 6-keto-PGF 1a ), dihomo-PG (1a,1b-dihomo-PGF 2a ) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA), with purities !95% as well as deuterated internal standards (IS, PGF 2a -d 4 and 15-F 2t -IsoP-d 4 ) with purities !98% and incorporation !99% deuterated form (d 1 -d 4 ); <1% d 0 .
Other analytical standards employed were F 2 -IsoPs (5-F 2t -IsoP, 5-epi-5-F 2t -IsoP, 15-epi-2,3-dinor-15-F 2t -IsoP), F 4 -NeuroPs (4-F 4t -NeuroP, 4-epi-4-F 4t -NeuroP, 10-epi-10-F 4t -NeuroP, 10-F 4t -NeuroP, 14(RS)-14-F 4t -NeuroP), Potassium hydroxide (KOH) was from Sigma Aldrich Química SA (Madrid, Spain). LC-MS grade solvents (methanol CH 3 OH, ethanol, heptane and acetonitrile CH 3 CN) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Formic acid (HCOOH, 98%) and ethyl acetate (analytical grade) were from Panreac (Barcelona, Spain). Ultrapure H 2 O was generated on a milliQ system from Merck Millipore (Darmstadt, Germany).
Other analytical standards employed were F 2 -IsoPs (5-F 2t -IsoP, 5-epi-5-F 2t -IsoP, 15-epi-2,3-dinor-15-F 2t -IsoP), F 4 -NeuroPs (4-F 4t -NeuroP, 4-epi-4-F 4t -NeuroP, 10-epi-10-F 4t -NeuroP, 10-F 4t -NeuroP, 14(RS)-14-F 4t -NeuroP), Potassium hydroxide (KOH) was from Sigma Aldrich Química SA (Madrid, Spain). LC-MS grade solvents (methanol CH 3 OH, ethanol, heptane and acetonitrile CH 3 CN) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Formic acid (HCOOH, 98%) and ethyl acetate (analytical grade) were from Panreac (Barcelona, Spain). Ultrapure H 2 O was generated on a milliQ system from Merck Millipore (Darmstadt, Germany).
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