The activity of caspase-3 was measured using the Caspase 3/7 activity assay kit (AAT Bioquest, Inc., USA) according to the manufacturer’s instruction. Briefly, 5 x 104 cells were plated in each well of 96-well plates and incubated overnight in a 5% CO2 incubator. The cells were treated with rJHP0290 (100 ng/ml) and/or CPT (10 μM), as indicated, for 16 h, followed by the addition of 100 μl/well of caspase 3 assay solution. The plate was incubated at room temperature for 1 h, protected from light. The fluorescence intensity was measured at Ex/Em = 350/450 nm using a POLARstar Omega Instrument (BMG Labtech).
Polarstar omega instrument
Manufactured by BMG Labtech
The POLARstar Omega Instrument is a multi-mode microplate reader designed for high-performance detection and quantification of various assays. It is capable of measuring absorbance, fluorescence, and luminescence in microplates. The instrument is equipped with a temperature-controlled incubator and shaker to provide optimal conditions for cell-based and biochemical assays.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using polarstar omega instrument
1
Caspase-3 Activity Assay Protocol
2
Luciferase Activity Assay for HCV Replicon
3
Caspase-3 Activity Measurement in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Caspase-3 activity was measured using the Caspase 3/7 activity assay kit (AAT Bioquest, Inc., USA) following the manufacturer's instructions. Briefly, 5 x 104 cells were challenged with rHP1286 (1 μg/ml) or E. coli LPS (500 ng/ml) for 24 h followed by the addition of 100 μl/well of caspase 3 assay solution. The fluorescence intensity was measured (Ex/Em = 350/450 nm) after 1 h incubation. POLARstar Omega Instrument (BMG Labtech) was used for the data acquisition.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!