Samples were taken from cardiac tissue around the infarct region and fixed in 4% formalin for 24 hours before cryopexy. Left ventricular sections (5 μm) were stained with hematoxylin‐eosin. In addition, the collagen density was assessed using Masson trichrome stain, as previously described.23 The total area of fibrosis was analyzed quantitatively using a BZ‐X710 all‐in‐one microscope (KEYENCE, Osaka, Japan). In immunohistochemistry stains using the neutrophil‐specific maker Gr‐1 (Ly‐6G; #550291, 1:10, BD BioScience, SanJose, CA), we analyzed neutrophil infiltrations on hearts from 3 mice in each group. Secondary antibodies consisted of biotinylated anti‐rat antibodies, followed with streptavidin‐peroxidase staining kit (Histofine Simple Stain MaxPO Multi, Nichirei, Tokyo, Japan) and visualized with a peroxidase substrate solution containing 3,3′‐diaminobenzidine (Simple Stain DAB, Nichirei, Tokyo, Japan).
Bz x710 all in one microscope
Manufactured by Keyence
Sourced in Japan
The BZ‐X710 all‐in‐one microscope is a compact and versatile imaging system designed for a wide range of applications. It features a high-resolution camera, advanced optics, and intuitive software for capturing and analyzing digital images. The core function of the BZ‐X710 is to provide users with a comprehensive microscopy solution for their research and analysis needs.
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Lab products found in correlation
2 protocols using bz x710 all in one microscope
1
Histopathological Assessment of Cardiac Fibrosis
2
Time-lapse Analysis of Cell Cycle
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To carry out time-lapse analysis, HeLa cells were arrested at the G1/S boundary by treating them with 2.5 mM of thymidine for 18 h, releasing them for 9 h, and then treating them again with the same dose of thymidine for another 18 h. The resulting cells were washed with fresh medium, released from the double-thymidine block for 7 h, and then treated with control DMSO or 10 μM of Allopole to examine their effect on cell cycle progression. At the indicated time points, cells were imaged every 30 min using a Keyence BZ-X710 all-in-one microscope.
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