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Rabbit anti pax6

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Rabbit anti-Pax6 is a polyclonal antibody that specifically targets the Pax6 protein. Pax6 is a transcription factor that plays a crucial role in the development and function of the eye, brain, and other organs. This antibody can be used for various research applications, such as protein detection and localization studies.

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Lab products found in correlation

Rabbit anti tbr2, by Abcam (6 mentions) Goat anti sox2, by Santa Cruz Biotechnology (4 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (4 mentions) Mouse anti nestin, by Merck Group (4 mentions) Axiovert 200m, by Zeiss (4 mentions) Mouse anti gaba, by Merck Group (3 mentions) Mouse anti tra 1 81, by Merck Group (3 mentions) Mouse anti tra 1 60, by Merck Group (3 mentions) Rabbit anti tuj1, by Fortrea (3 mentions) Mouse anti tuj1, by Fortrea (3 mentions) Mouse anti blbp, by Abcam (3 mentions) Mouse anti nestin, by Abcam (3 mentions) Rabbit anti ki67, by Abcam (3 mentions) Rabbit anti glutamate, by Merck Group (3 mentions) Rabbit anti vglut1, by Synaptic Systems (3 mentions) Mouse anti map2, by Merck Group (3 mentions) Mouse anti nkx2, by Merck Group (3 mentions) Alexa fluor 488, by Thermo Fisher Scientific (3 mentions) Lsm 710 confocal microscope, by Zeiss (3 mentions) Axio observer z1, by Zeiss (3 mentions)

24 protocols using rabbit anti pax6

1

Immunostaining and Western Blot Analysis

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Immunostaining and WB were performed as described (Chen et al., 2019 (link)). The following antibodies were used: mouse anti-Oct4 (Millipore), goat anti-Sox2 (Santa Cruz), mouse anti-Tra1-81 (Millipore), mouse anti-Tra1-60 (Millipore), mouse anti-Nestin (Chemicon), rabbit anti-Tuj1 (Covance), mouse anti-Tuj1 (Sigma), mouse anti-MAP2 (Sigma), rabbit H3K27me3 (Santa Cruz), rabbit anti-Pax6 (Covance), mouse anti-GABA (Sigma), mouse anti-Nkx2.1 (Millipore), rabbit anti-Tbr1 (Chemicon), mouse anti-vGluT1 (Millipore). MeCP2 antibody used in Western blot was a gift from Dr. Keping Hu.
Chen X., Han X., Blanchi B., Guan W., Ge W., Yu Y.C, & Sun Y.E. (2020). Graded and pan-neural disease phenotypes of Rett Syndrome linked with dosage of functional MeCP2. Protein & Cell, 12(8), 639-652.
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2

Immunostaining of Cryosectioned Tissues

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Histological and immunofluorescence staining on cryosections was performed as described previously (de Melo et al., 2005 (link)). Primary antibodies used were: mouse anti-BrdU (1:200, Chemicon), mouse anti-BRN3A (1:200, Santa Cruz), goat anti-BRN3B (1:200, Santa Cruz), rabbit anti-caspase 3 (1:500, Cell Signaling Technologies), rabbit anti-DLX2 (1:400, C199 affinity purified), mouse anti-ISLET1 (1:600, DSHB, University of Iowa), rabbit anti-phosphohistone H3 (1:1000, Upstate), rabbit anti-PROX1 (1:500, Chemicon), rabbit anti-PAX6 (1:800, Covance) and mouse anti-syntaxin (1:6000, Sigma). Secondary antibodies and fluorescent tertiary molecules used were: FITC-conjugated goat anti-rabbit (1:200), biotin-SP-conjugated goat anti-rabbit (1:200), biotin-SP-conjugated goat anti-mouse (1:200) (Jackson ImmunoResearch), streptavidin-conjugated Oregon Green 488 (1:200) and streptavidin-conjugated Texas Red (1:200) (Molecular Probes). Negative controls omitted the primary antibody. TUNEL staining used the In Situ Cell Death Detection Kit, TMR Red (Roche Diagnostics). Non-radioactive digoxigenin in situ RNA hybridization was performed as described previously (de Melo et al., 2005 (link)).
Zhang Q., Zagozewski J., Cheng S., Dixit R., Zhang S., de Melo J., Mu X., Klein W.H., Brown N.L., Wigle J.T., Schuurmans C, & Eisenstat D.D. (2017). Regulation of Brn3b by DLX1 and DLX2 is required for retinal ganglion cell differentiation in the vertebrate retina. Development (Cambridge, England), 144(9), 1698-1711.
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3

Immunostaining of E9.5-11.5 Mouse Embryos

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E9.5–11.5 embryos were dissected and prepared for cryosectioning using standard protocols [40 (link)]. Cryosections were stained with primary antibodies and fluorescent secondary antibodies (Jackson Immunologicals), followed by counterstaining with DAPI. Primary antibodies were: mouse anti-Shh 5E1, mouse anti-Nkx2.2, mouse anti-HB9, mouse anti-Isl1/2, mouse anti-FoxA2, mouse anti-Nkx6.1, mouse anti-Pax7 (all from DSHB at 1:10), and sheep anti-Chx10 (1:600, Exalpha), and rabbit anti-Pax6 (1:300, Covance).
Snouffer A., Brown D., Lee H., Walsh J., Lupu F., Norman R., Lechtreck K., Ko H.W, & Eggenschwiler J. (2017). Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice. PLoS Genetics, 13(8), e1006912.
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4

Immunofluorescence Staining of Developing Cortex

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Perfusion, dissection, and immunofluorescence staining were conducted according to standard protocols as previously described [17 (link)]. The following are the antibodies used: mouse anti-BrdU (1:50 dilution; BD Pharmingen, Franklin Lakes, NJ, USA), rabbit anti-Cux1 (1:100 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-Phospho-Histone H3 (1:250 dilution; Millipore, Billerica, MA, USA), rabbit anti-Pax 6 (1:500 dilution; Covance, Princeton, NJ, USA), rabbit anti-Tbr2 (1:500 dilution; Abcam, Cambridge, UK), mouse anti-Tuj1 (1:500 dilution; Covance, Princeton, NJ, USA), rabbit anti-Gli3 (1:100 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit anti-Cleaved Caspase 3 (1:300 dilution; Cell Signaling, Madison, WI, USA).
For 5-bromo-2-deoxyuridine (BrdU, Sigma, St. Louis, MO, USA) labeling, pregnant dams were treated with 50 μg/g BrdU by intraperitoneal injection for 4 h prior to dissection at E16.5. DiI labeling was conducted by placing small crystals of the lipophilic tracer (1,1′-dioctadecyl-3,3,3′, 3′-tetramethylindocarbocyanine; Invitrogen, Waltham, MA, USA) in the neocortex to target the upper layer (2/3) and then remained in 4% paraformaldehyde (PFA). After 6 weeks, brains were sectioned at 100 μm, counterstained with bisbenzimide, mounted, and imaged.
Yabut O.R., Ng H.X., Fernandez G., Yoon K., Kuhn J, & Pleasure S.J. (2016). Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors. Journal of Developmental Biology, 4(4), 29.
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5

Immunocytochemistry of iPSC-Derived Sensory Neurons

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Immunocytochemistry was performed on iPSC‐SNs seeded in 96‐well plates or µ‐slide chambers. Cells were fixed with 4% paraformaldehyde in phosphate‐buffered saline (PBS) for 15 minutes, permeabilized with PBS containing 0.1% Triton X‐100 for 10 minutes, blocked with 5% goat serum in PBS for 1 hour, and incubated overnight at 4°C with one of the following primary antibodies: rabbit anti‐PAX6 (1:500; Covance), mouse/rabbit anti‐Tubulin βIII clone TUJ1 (1:1,000; Covance), goat anti‐SOX10 (1:500; Santa Cruz), mouse anti‐BRN3A (1:500; Millipore), goat anti‐Peripherin (1:500; Santa Cruz), pig anti‐TRPV1 (1:200; ThermoFisher), and rabbit anti‐TrkA/B/C (1:50; Alomone Labs). Following several washes, the corresponding Alexa Fluor 488/568/594‐conjugated secondary antibodies (1:1,000; ThermoFisher) were added for 1 hour at room temperature. Cells were incubated with DAPI (1:1,000; ThermoFisher) for 10 minutes to visualize nuclei. A list of the primary and secondary antibodies used in this study is provided in Table S2.
Xiong C., Chua K.C., Stage T.B., Priotti J., Kim J., Altman‐Merino A., Chan D., Saraf K., Canato Ferracini A., Fattahi F, & Kroetz D.L. (2020). Human Induced Pluripotent Stem Cell Derived Sensory Neurons are Sensitive to the Neurotoxic Effects of Paclitaxel. Clinical and Translational Science, 14(2), 568-581.
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6

Immunocytochemistry and Western Blot Protocols

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Primary antibodies used for immunocytochemistry or Western blot include mouse anti-GAPDH (Millipore), mouse anti-Nestin (Millipore), mouse anti-Tuj1/βIII-tubulin (Millipore), mouse anti-Tuj1/βIII-tubulin (Santa Cruz Biotenchnology), mouse anti-TRA-1-60 (Millipore), mouse anti-TRA-1-81 (Millipore), goat anti-Lin28a (R&D Systems), rabbit anti-Nanog (Santa Cruz Biotechnology), rabbit anti-Oct3/4 (Santa Cruz Biotechnology), rabbit anti-DCX44 (link), goat anti-DCX (Santa Cruz Biotechnology), goat anti-SOX2 (Santa Cruz Biotechnology), rabbit anti-PAX6 (Covance), mouse anti-MAP2 (Millipore), mouse anti-BLBP (Abcam), rabbit anti-GFAP (Sigma), rabbit anti-OLIG2 (Abcam), mouse anti-β-Catenin (BD), rabbit anti-Arp3 (Santa Cruz Biotechnology), mouse anti-β-actin (Sigma), rabbit anti-EIF3D (Bethyl Labs), rat anti-αN-catenin obtained from the NIH NICHD Developmental Studies Hybridoma Bank (Catalog: NCAT2, deposited by Masatoshi Takeichi and Shinji Hirano). Fluorophore conjugated secondary antibodies were purchased from Invitrogen, and immunohistochemistry was performed with ABC Elite peroxidase staining kit, using ImmpactDAB as a substrate.
Schaffer A.E., Breuss M.W., Caglayan A.O., Al-Sanaa N., Al-Abdulwahed H.Y., Kaymakçalan H., Yılmaz C., Zaki M.S., Rosti R.O., Copeland B., Baek S.T., Musaev D., Scott E.C., Ben-Omran T., Kariminejad A., Kayserili H., Mojahedi F., Kara M., Cai N., Silhavy J.L., Elsharif S., Fenercioglu E., Barshop B.A., Kara B., Wang R., Stanley V., James K.N., Nachnani R., Kalur A., Megahed H., Incecik F., Danda S., Alanay Y., Faqeih E., Melikishvili G., Mansour L., Miller I., Sukhudyan B., Chelly J., Dobyns W.B., Bilguvar K., Jamra R.A., Gunel M, & Gleeson J.G. (2018). Bi-allelic loss of human CTNNA2, encoding αN-catenin, leads to ARP2/3 over-activity and disordered cortical neuronal migration. Nature genetics, 50(8), 1093-1101.
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7

Cortical Development Immunofluorescence Staining

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Perfusion, dissection, and immunofluorescence staining were conducted according to standard protocols as previously described [17 (link)]. The following are the antibodies used: mouse anti-BrdU (1:50 dilution; BD Pharmingen, Franklin Lakes, NJ, USA), rabbit anti-Cux1 (1:100 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-Phospho-Histone H3 (1:250 dilution; Millipore, Billerica, MA, USA), rabbit anti-Pax 6 (1:500 dilution; Covance, Princeton, NJ, USA), rabbit anti-Tbr2 (1:500 dilution; Abcam, Cambridge, UK), mouse anti-Tuj1 (1:500 dilution; Covance, Princeton, NJ, USA), rabbit anti-Gli3 (1:100 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), and rabbit anti-Cleaved Caspase 3 (1:300 dilution; Cell Signaling, Madison, WI, USA).
For 5-bromo-2-deoxyuridine (BrdU, Sigma, St. Louis, MO, USA) labeling, pregnant dams were treated with 50 μg/g BrdU by intraperitoneal injection for 4 h prior to dissection at E16.5. DiI labeling was conducted by placing small crystals of the lipophilic tracer (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine; Invitrogen, Waltham, MA, USA) in the neocortex to target the upper layer (2/3) and then remained in 4% paraformaldehyde (PFA). After 6 weeks, brains were sectioned at 100 μm, counterstained with bisbenzimide, mounted, and imaged.
Yabut O.R., Ng H.X., Fernandez G., Yoon K., Kuhn J, & Pleasure S.J. (2016). Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors. Journal of Developmental Biology, 4(4), 29.
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8

Antibody Staining Panel for Neural Development

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Primary antibodies used included rabbit anti-Kif2a (1: 1000, Abcam), rabbit anti-Tbr2 (1:500, Abcam), rabbit anti-Pax6 (1:200, Covance), mouse anti-beta III tubulin (TU20) (1:500, Abcam), mouse anti-nestin (1:500, Abcam), mouse anti-α tubulin (1:5000, CST), rat anti-Brdu (1:500, Abcam), rabbit and chicken anti-green fluorescent protein (1:500, GFP) (Invitrogen and Aves, respectively), rabbit anti-Ki67 (1:500, Thermo), rabbit anti-PH3 (1:300, Millipore), rabbit anti-cleaved caspase3 (1:300, Cell signaling), rabbit anti-AKT (1:500, CST), rabbit anti-p-AKT (1:500, CST), rabbit anti-β-catenin (1:200, Santa Cruz), mouse anti-GSK3β (1:200, Santa Cruz), and mouse anti-p-GSK3β (1:200, Santa Cruz). All the corresponding conjugated secondary antibodies were purchased from Invitrogen. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Roche).
Sun D., Zhou X., Yu H.L., He X.X., Guo W.X., Xiong W.C, & Zhu X.J. (2017). Regulation of neural stem cell proliferation and differentiation by Kinesin family member 2a. PLoS ONE, 12(6), e0179047.
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9

Comprehensive Immunostaining and In Situ Hybridization

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Immunostaining and in situ hybridization were performed as previously described [47 (link)]. The Fezf2 cRNA probe corresponds to nucleotides 644 to 1,374 of mouse Fezf2 (GenBank: NC_000080). LacZ staining was preformed according to standard protocols. Primary antibodies used: Chicken anti β-gal (Abcam, 1:500), Rabbit anti BLBP (Millipore, 1:500), Rat anti CTIP2 (Abcam, 1:1,000), Guinea Pig anti GFAP (Advaned Immuno Chemical, 1:100), Rabbit anti PAX6 (Covance, 1:100), PHH3 (Cell Signaling, 1:100), Rabbit anti SATB2 (Abcam, 1:1,000), Goat anti SOX2 (Santa Cruz Biotech, 1:500), Rabbit anti SOX5 (Abcam, 1:500), Rabbit anti TBR1 (Abcam, 1:1,000), Mouse anti Tuj1 (Covance, 1:1,000). Primary antibodies were detected using AlexaFluor-conjugated secondary antibodies (Invitrogen, 1:1,000). DNA was visualized with DAPI (1:50,000).
Eckler M.J., Larkin K.A., McKenna W.L., Katzman S., Guo C., Roque R., Visel A., Rubenstein J.L, & Chen B. (2014). Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex. Neural Development, 9, 6.
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10

Comprehensive Antibody Immunostaining Protocol

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The primary antibodies used were mouse anti-GFP (Invitrogen—1:1000), rabbit anti-GFP (Abcam—1:5000), chicken anti-GFP (Millipore—1:1000), mouse anti-SatB2 (Abcam—1:400), mouse anti-βIII-tubulin (Covance—1:1000), rabbit anti-βIII-tubulin (Covance—1:1000), rabbit anti-Pax6 (Covance—1:2000), mouse anti-Pax6 (Abcam—1:250), rabbit anti-GAPDH (Sigma—1:1000), rabbit anti-Tbr2 (Abcam—1:500), rabbit anti-Erk (Santa Cruz Biotechnology—1:1000), rabbit anti-Notch1 (Abcam—1:250), rabbit anti-RFP (MBL—1:1000), mouse anti-Actin (Sigma—1:2000), mouse anti-MG-H1 (Cell Biolabs—1:50), mouse anti-GAPDH (Abcam—1:1000), rabbit anti-GLO1 (Abcam 1:500), mouse anti-puromycin (Kerafast—1:1000). The donkey anti-mouse and anti-rabbit Alexa 488, 555 and 647-conjugated secondary antibodies were obtained from ThermoFisher and used at 1:500 dilutions. HRP-conjugated goat anti-mouse, anti-rabbit or anti-chicken secondary antibodies were purchased from ThermoFisher and used at 1:5000 dilutions. NIR-conjugated goat anti-mouse and anti-rabbit secondary antibodies were acquired from LI-COR and used at 1:25,000 dilutions.
Rodrigues D.C., Harvey E.M., Suraj R., Erickson S.L., Mohammad L., Ren M., Liu H., He G., Kaplan D.R., Ellis J, & Yang G. (2020). Methylglyoxal couples metabolic and translational control of Notch signalling in mammalian neural stem cells. Nature Communications, 11, 2018.
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