Rabbit anti card19 hpa010990

Manufactured by Atlas Antibodies

Sourced in United States

Rabbit anti-CARD19 (HPA010990) is a primary antibody produced in rabbit targeting the CARD19 protein. It is intended for use in immunohistochemistry and western blot applications.

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Lab products found in correlation

Mouse anti flag m2, by Merck Group (2 mentions) Alexa fluor 488 goat anti mouse igg1, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse igg2a, by Thermo Fisher Scientific (1 mentions) Rabbit anti tomm20, by Santa Cruz Biotechnology (1 mentions) Rabbit anti ha y11, by Santa Cruz Biotechnology (1 mentions) Mouse anti gfp clone 3e6, by MP Biomedicals (1 mentions) Mouse anti gapdh 6c5, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin c4, by Santa Cruz Biotechnology (1 mentions) Rabbit anti iκbα, by Cell Signaling Technology (1 mentions)

2 protocols using rabbit anti card19 hpa010990

Antibodies for Immunoblotting and Microscopy

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Antibodies employed for immunoblotting and microscopy are as follows: rabbit anti-Myc (Bethyl), rabbit anti-TOMM20 (FL-145), mouse anti-β-actin (C-4), mouse anti-MAVS (E3), mouse anti-Mfn2 (XX-1), mouse anti-calnexin (E-10), mouse anti-Tim23 (H-8) (all from Santa Cruz, CA, USA), mouse anti-FLAG (M2) (Sigma-Aldrich, St. Louis, MO, USA), rabbit anti-CARD19 (HPA010990), rabbit anti-SAMM50 (HPA034537) (Atlas Antibodies, Stockholm, Sweden), mouse anti-mitofilin (2E4AD5), mouse anti-ATP synthase β (4.3E8.D10) (both from Thermo Fisher, Waltham, MA, USA), and rat anti-OLLAS ([32 (link)]; purified from hybridoma supernatant). Secondary antibodies for microscopy were goat anti-rabbit (H + L) Alexa Fluor 647, goat anti-mouse (IgG1) Alexa Fluor 488 and goat anti-mouse (IgG2a) Alexa Fluor 488 (all from Thermo Fisher, Waltham, MA, USA), used at a 1:200 dilution.

Rios K.E., Zhou M., Lott N.M., Beauregard C.R., McDaniel D.P., Conrads T.P, & Schaefer B.C. (2022). CARD19 Interacts with Mitochondrial Contact Site and Cristae Organizing System Constituent Proteins and Regulates Cristae Morphology. Cells, 11(7), 1175.

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Analysis of Protein Complexes in HEK-293T Cells

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Transiently transfected HEK-293T cells were lysed and analyzed by western blotting as previously described [15 (link)]. The following antibodies were used: rabbit anti-HA (Y-11); rabbit anti-Bcl10 (H-197); mouse anti-GAPDH (6C5); mouse anti-A20 (A12); rabbit anti-TOMM20; mouse anti-β-actin (C-4); (all from Santa Cruz), rabbit anti-FLAG; mouse anti-FLAG (M2) (Sigma-Aldrich); rabbit anti-phospho-IκBα (Ser32) (14D4); rabbit anti- IκBα (Cell Signaling Technologies); rabbit anti-CARD19 (HPA010990) (Atlas Antibodies); mouse anti-MCPIP1 (anti-Regnase) (MAB7875) (R&D Systems); and mouse anti-α-tubulin (purified from DSHB hybridoma AA4.3 supernatant; AA4.3 Hybridoma deposited by Walsh, C). Immunoprecipitation of Bcl10-GFP protein complexes was performed with 1 μg/mL mouse anti-GFP clone 3E6 (MP Biomedicals), as previously described [15 (link)].

Rios K.E., Kashyap A.K., Maynard S.K., Washington M., Paul S, & Schaefer B.C. (2020). CARD19, the protein formerly known as BinCARD, is a mitochondrial protein that does not regulate Bcl10-dependent NF-κB activation after TCR engagement.. Cellular immunology, 356, 104179.

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