Distearoyl sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

Distearoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid commonly used in research applications. It is a structural component of biological membranes and serves as a precursor for the synthesis of other phospholipids.

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Lab products found in correlation

Dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) Megaclear transcription clean up kit, by Thermo Fisher Scientific (1 mentions) Cholesterol, by Avanti Polar Lipids (1 mentions) Megascript t7 transcription kit, by Thermo Fisher Scientific (1 mentions) Detoxi gel endotoxin removing column, by Thermo Fisher Scientific (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Poly lactic co glycolic acid plga, by Evonik (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Rpmi 1640 cell culture media, by American Type Culture Collection (1 mentions) Dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Dilinoleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Dimethyldioctadecylammonium chloride, by Merck Group (1 mentions) Zetasizer nano zs90, by Malvern Panalytical (1 mentions)

Close spelling variants of this product

1,2-distearoyl-sn-glycero-3-phosphocholine (81 citations) 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (48 citations) 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol (5 citations) -distearoyl-sn-glycero-3-phosphocholine (DSPC (3 citations) 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG2000), (3 citations) 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phospho-ethanol amine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000), (2 citations) C12-200, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC, (2 citations)

5 protocols using distearoyl sn glycero 3 phosphocholine

Lipid Composition for Nanoparticle Formulations

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1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-ditetradecanoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PEG2000-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)2000] (Biotin-PEG2000-DSPE), 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine-N-(Lissamine Rhodamine B Sulfonyl) (Rhodamine PE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (PEG2000-DSPEmal), 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL). Gd(III)DOTA-DSPE and Gd(III)-DTPA-bis(stearylamide) (Gd(III)-DTPA-BSA) were obtained from Gateway Chemical Technology (St. Louis, MO).

Kuijten M.M., Hannah Degeling M., Chen J.W., Wojtkiewicz G., Waterman P., Weissleder R., Azzi J., Nicolay K, & Tannous B.A. (2015). Multimodal targeted high relaxivity thermosensitive liposome for in vivo imaging. Scientific Reports, 5, 17220.

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Lipid Nanoparticle Formulation for mRNA Delivery

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Cholesterol, distearoyl-sn-glycero-3-phosphocholine (DSPC), and dimyristoyl-rac-glycero-3-methoxypolyethylene glycol (PEG-DMG) were from Avanti Polar Lipids (Alabaster, AL, USA). The proprietary ionizable lipid (lipid 14) was synthesized in-house by G. S. Naidu (fig. S3) (48 (link)). mRNA sequences were purchased from TriLink (San Diego, CA, USA) or synthesized by an in vitro transcription (IVT) reaction using the MEGAscript T7 transcription kit and cleaned by the MEGAclear transcription clean-up kit both from Thermo Fisher Scientific (Waltham, MA, USA). All mRNA sequences were synthesized with complete N1-methyl-pseudouridine nucleotide substitution.

Kon E., Levy Y., Elia U., Cohen H., Hazan-Halevy I., Aftalion M., Ezra A., Bar-Haim E., Naidu G.S., Diesendruck Y., Rotem S., Ad-El N., Goldsmith M., Mamroud E., Peer D, & Cohen O. (2023). A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium. Science Advances, 9(10), eadg1036.

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Lipid-based Vaccine Formulation Protocol

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All
lipids, 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), 1,2-dioleoyl-sn-glycero-3-phoshpo-(1′-rac-gylcerol)
(DOPG), 1,2-distearoyl-sn-glycero-3-phosphocholine
(DSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(poly(ethylene
glycol))2000] (DSPE-PEG2K-maleimide), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine
B sulfonyl) (14:0 Liss-Rhod-DOPE), 1,2-distearoyl-sn-glycero-3-phospohethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl)
(NBD-DSPE), and cholesterol, were purchased from Avanti Polar Lipids
(Alabaster, AL). Poly(lactic-co-glycolic acid) (PLGA)
with a 50:50 ratio of lactic acid and glycolic acid and an inherent
viscosity of 0.42 dL/G was purchased from Evonik Corporation (Birmingham,
AL). Monophosphoryl lipid A (MPLA, from Salmonella
enterica serotype minnesota Re 595, cat. no. L6895)
and solvents were purchased from Sigma-Aldrich (St. Louis, MO). N-Succinimidyl S-acetyl(thiotetraethylene
glycol) (SAT(PEG)₄) was purchased from Pierce Biotechnology
(Rockford, IL). Purified ovalbumin (OVA) was purchased from Worthington
Biochemical (Lakewood, NJ) and subsequently passed through detoxi-gel
endotoxin-removing columns (Pierce Biotechnology, Rockford, IL) to
remove any trace endotoxin.

Hanson M.C., Bershteyn A., Crespo M.P, & Irvine D.J. (2014). Antigen Delivery by Lipid-Enveloped PLGA Microparticle Vaccines Mediated by in Situ Vesicle Shedding. Biomacromolecules, 15(7), 2475-2481.

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Phospholipid Characterization for Cell Studies

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1,2-dilinoleoyl-sn-glycero-3-phosphocholine (14:1),
1,2-dipalmitoleoyl-sn-glycero-3-phosphocholine (16:1),
1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC/18:1 cis),
1,2-dielaidoyl-sn-glycero-3-phosphocholine (18:1 trans),
1,2-dieicosenoyl-sn-glycero-3-phosphocholine (20:1),
1,2-dierucoyl-sn-glycero-3-phosphocholine (22:1),
1,2-dinervonoyl-sn-glycero-3-phosphocholine (24:1),
1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC/14:0),
1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC/16:0),
1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC/18:0),
1,2-dilinoleoyl-sn-glycero-3-phosphocholine (18:2), and
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC/16:0–18:1 cis)
were purchased from Avanti Polar Lipids (Alabaster, AL). All other reagents were
ordered from Sigma-Aldrich (St. Louis, MO). RPMI-1640 cell culture media was
purchased from ATCC (Manassas, VA). Fetal bovine serum, Penicillin/Streptomycin,
and Alexa Fluor 488 NHS Ester (AF488) were obtained from Invitrogen (Carlsbad,
CA).

Gilmore S.F., Carpenter T.S., Ingólfsson H.I., Peters S.K., Henderson P.T., Blanchette C.D, & Fischer N.O. (2018). Lipid composition dictates serum stability of reconstituted high-density lipoproteins: implications for in vivo applications. Nanoscale, 10(16), 7420-7430.

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Targeted siRNA Delivery via Nanoliposomes

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siRNA targeting survivin was synthesized by Biomics Biotechnologies Co., Ltd. (Nantong, China), with a sense sequence of 5′-GCAUCUCUACAUUCAAGAAdTdT-3′ and an antisense sequence of 5′-UUCUUGAAUGUAGAGAUGCdTdT-3′. An unrelated silencing sequence was synthesized as a negative control (NC), and the sequences of NC siRNA were as follows: Sense, 5′-UUCUCCGAACGUGUCACGUdTdT-3′; antisense, 5′-ACGUGACACGUUCGGAGAAdTdT-3′. siRNA molecules were encapsulated into nanoliposomes in ethanol-water solutions with a lipid composition of 1,2-distearoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Inc., Alablaster, AL, USA), cholesterol, dimethyldioctadecylammonium chloride and poly(ethylene glycol) ceramide C16 (all from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany) in 100% ethanol (Sigma-Aldrich; Merck Millipore) at a molar ratio of 25:45:25:2.5, respectively. The mean diameter of the nanoliposomes was determined using a nanoparticle size analyzer (Zetasizer Nano ZS90; Malvern Instruments Ltd., Malvern, UK) and the data was 70.7±29.077 nm for survivin siRNA and 64.9±26.128 nm for NC siRNA.

Liu Z., Wang T., Zhang Z., Tang S., Feng S., Yue M., Hu M., Xuan L, & Chen Y. (2017). Survivin downregulation using siRNA nanoliposomes inhibits cell proliferation and promotes the apoptosis of MHCC-97H hepatic cancer cells: An in vitro and in vivo study. Oncology Letters, 13(4), 2723-2730.

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