Gapdh antibody 10r g109a

All cell culture mediums and trypsin were purchased from Life Technology Corp. (Carlsbad, CA). Oridonin and antibody against α-smooth muscle actin (α-SMA) (Cat#5228) were purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO). TGF-β1 was purchased from R&D Systems Inc. (Minneapolis, MN). Propidium iodide was from MP Biomedicals, LLC (Solon, OH). Antibodies against Fibronectin (sc-6952) and Cdk4 (sc-749) were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA). Anti-Collagen Type I polyclonal antibody (600-401-103) was from Rockland Immunochemicals Inc. (Gilbertsville, PA). GAPDH antibody (10R-G109A) was from Fitzgerald Industries (Concord, MA). Anti-p21 (Cat#556431) and p16 (Cat#551153) were from BD Biosciences (San Jose, CA). Anti-p53 (Cat#2527), cleaved PARP (Cat#5625), cleaved caspase-9 (Cat#9505) and phospho-Smad2/3 (Cat#8828) were from Cell Signaling Technology Inc. (Danvers, MA).

Whole cell extracts were prepared as previously described [18 (link)]. Antibody against α-smooth muscle actin (α-SMA) (Cat#5228) was purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO). Anti-Collagen Type I polyclonal antibody (600-401-103) was purchased from Rockland Immunochemicals Inc. (Gilbertsville, PA). Antibodies against Fibronectin (sc-6952) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). GAPDH antibody (10R-G109A) was purchased from Fitzgerald Industries (Concord, MA). Anti-p21 (Cat#556431) was purchased from BD Biosciences (San Jose, CA). Anti-p53 (Cat#2527), phospho-p53 (Cat#9286), and phospho-Smad2/3 (Cat#8828) were purchased from Cell Signaling Technology Inc. (Danvers, MA). Apoptosis Western Blot Cocktail (ab136812) was purchased from Abcam (Cambridge, MA).

All cell culture mediums and trypsin were purchased from Life Technology Corp. (Carlsbad, CA). Oridonin and antibody against α-smooth muscle actin (α-SMA) (Cat#5228) were purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO). Transforming growth factor β1 (TGFβ1) was purchased from R&D Systems Inc. (Minneapolis, MN). Propidium iodide was purchased from MP Biomedicals, LLC (Solon, OH). Antibodies against Fibronectin (sc-6952) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Anti-Collagen Type I polyclonal antibody (600-401-103) was purchased from Rockland Immunochemicals Inc. (Gilbertsville, PA). GAPDH antibody (10R-G109A) was purchased from Fitzgerald Industries (Concord, MA). Anti-p21 (Cat#556431) was purchased from BD Biosciences (San Jose, CA). Anti-p53 (Cat#2527), phospho-p53 (Cat#9286), cleaved PARP (Cat#5625), cleaved caspase-9 (Cat#9505), phospho-ERK 1/2 (Cat#4377) and phospho-Smad 2/3 (Cat#8828) were purchased from Cell Signaling Technology Inc. (Danvers, MA). Cleaved caspase-3 (ab136812) was purchased from Abcam plc. (Cambridge, MA).
CYD0692 is a novel analogue designed with an additional α-formylenone motif formed in the A-ring and an acetonide moiety introduced at 7,14-dihydroxyl of Oridonin (Figure 1). CYD0692 was synthesized following our previously reported protocols [13 (link)].