Dm400b digital microscope

Manufactured by Leica

The Leica DM400B is a digital microscope designed for high-quality imaging and analysis. It features a high-resolution digital camera and advanced software for capturing and processing images. The DM400B is capable of magnifying samples and providing detailed, accurate information about their structure and composition.

Automatically generated - may contain errors

Lab products found in correlation

Sm2000r, by Leica (2 mentions) Cm 2000r, by Leica (1 mentions)

Spelling variants of this product

DM400B (35 citations) DM400B microscope (13 citations)

3 protocols using dm400b digital microscope

Brain Tissue Preparation for Microdialysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the microdialysis test session, rats were anesthetized using sodium pentobarbital (270 mg/kg; i.p.) and perfused intracardially with 50 mL of 0.9% saline, followed by 500 mL of 4% paraformaldehyde in 0.1 M phosphate buffer (PB). After being perfused, brains were removed, post-fixed in the same paraformaldehyde solution for 2 hours, then immersed in 20% sucrose and 0.01% sodium azide in 0.1 M PB for 48 hours at 4° C. Coronal sections (40 µm) were cut with a freezing microtome (SM 2000R; Leica), collected in PB, and mounted on to a slide immediately. Sections were imaged at 4x magnification using a Leica DM400B digital microscope to verify cannula placement.

Kawa A.B., Valenta A.C., Kennedy R.T, & Robinson T.E. (2019). Incentive and Dopamine Sensitization Produced by Intermittent But Not Long Access Cocaine Self-Administration. The European journal of neuroscience, 50(4), 2663-2682.

+ Open protocol

+ Expand

Brain Fixation and Sectioning Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the conclusion of the experiment, animals were anesthetized using sodium pentobarbital (270 mg/kg; i.p.) and perfused intracardially with 50 mL of 0.9% saline, followed by 500 mL of 4% paraformaldehyde in 0.1 m phosphate buffer (PB). After being perfused, brains were removed and post-fixed for 1 h at 4 °C in the same fixative, then immersed in 20% sucrose and 0.01% sodium azide in 0.1 m PB and stored at 4 °C. Coronal sections (40 μm) were cut with a freezing microtome (SM 2000R; Leica), collected in three parallel series in cryoprotectant solution (30% sucrose and 30% ethylene glycol in 0.1 m PB) and stored at −20 °C. Cresyl violet-stained sections were imaged at 4× magnification using a Leica DM400B digital microscope to verify probe placement.

Pitchers K.K., Kane L.F., Kim Y., Robinson T.E, & Sarter M. (2017). ‘Hot’ vs. ‘cold’ behavioural-cognitive styles: motivational-dopaminergic vs. cognitive-cholinergic processing of a Pavlovian cocaine cue in sign- and goal-tracking rats. The European journal of neuroscience, 46(11), 2768-2781.

+ Open protocol

+ Expand

Chronic Glutamate Recording in Rodent DMS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microelectrode arrays (MEAs) were chronically implanted into the DMS to record extracellular glutamate levels while performing the CTTT. Following the completion of experiments, administration of a lethal dose of sodium pentobarbital (270 mg/kg, i.p.) was followed by transcardial perfusion of saline, followed by 4% paraformaldehyde in 0.15 m sodium-phosphate solution, pH 7.4. Extracted brains were postfixed in 4% paraformaldehyde for 24 hrs, then submerged in 30% sucrose solution until they sank. Using a freezing microtome (CM 2000R; Leica), 35-μm thick brain slices were sectioned and stored in cryoprotectant until further histologic processing. Sections were mounted and processed with a Cresyl Violet Nissl stain to confirm MEAs placement. A Leica DM400B digital microscope was used to photomicrograph the sections at 1.25X and 5X magnification at three A-P levels (0.2 mm, 0.5 mm, and 1.0 mm).

Avila C, & Sarter M. (2024). Cortico-striatal action control inherent of opponent cognitive-motivational styles. bioRxiv.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!