Gsk3β ab93926

Low glucose Dulbecco's modified Eagle's medium (DMEM) (10567-014) and DMEM containing 4.5 g/L glucose (11965-092) were purchased from Gibco-BRL (Gaithersburg, MD). Dithizone (D5130) was from Sigma (St. Louis, MO). DMSO (042-21765) was from Wako (Tokyo, Japan). Recombinant human DKK-1 (5439-DK-010) and recombinant human Wnt-3a (5036-WN-010) was from R&D Inc. (Minneapolis, MN). Insulin antibody (sc-9168) was from Santa Cruz Biotechnology (Dallas, TX). C-peptide (ab14181) and GSK3β (ab93926) antibodies were from Abcam (Cambridge, UK). Phospho-GSK3β (Ser9) (D3A4) (9322S), non-phospho- (active) β-catenin (Ser33/37/Thr41) (D13A1) (8814S), and Dvl-2 (30D2) (3224P) antibodies were from Cell Signaling Technology (Beverly, MA). CD13 (BA0718), CD44 (BA0321), CD106 (BA0406), and CD49d (BA1640) antibodies were from Boster Biological (Wuhan, China). Pancreatic and duodenal homeobox-1 (PDX1, bs-0923R) antibody was from Bioss (Beijing, China). Cytokeratin 19 (CK19, BA2266) and nestin (BA1289) antibodies were from Boster Biological (Wuhan, China).

B16-F10 mouse melanoma cell line (ATCC^® CRL6475™) was purchased from the American Type Culture Collection (Manassas, VA, USA). Cytochalasin D, chlorpromazine hydrochloride, genistein, 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DOPG), and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were purchased from Sigma (St. Louis, MO, USA). Mouse monoclonal anti-glycogen synthase kinase-3β (GSK-3β) (ab93926, epitope within amino acids 292–420); and rabbit monoclonal anti-caspase-3 (ab184787, epitope within amino acids 1–175 recognizing total caspase-3) were purchased from Abcam (Cambridge, MA, USA). In addition, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Ibidi^TM µ-Dish 35 mm, high ibiditreat were purchased from Ibidi (Fitchburg, WI, USA). Hoechst 33342, modified Harris Hematoxylin (72711), and eosin (71304) were purchased from Thermo Scientific (Rockford, IL, USA). Rodent Block M, Mouse-on-Mouse HRP-Polymer, Background Sniper, and Rabbit-on-Rodent HRP Polymer were purchased from Biocare Medical, (Pacheco, CA, USA).