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Ethanol

Manufactured by Reanal
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Sourced in Hungary

Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It serves as a solvent, disinfectant, and fuel. Ethanol has the chemical formula C2H5OH and a molecular weight of 46.07 g/mol.

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Lab products found in correlation

Methanol, by Reanal (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Allyl bromide, by Merck Group (1 mentions) Sulfur, by Reanal (1 mentions) Sodium sulfide, by Reanal (1 mentions) Tetrabutylammonium iodide, by Merck Group (1 mentions) Anhydrous sodium carbonate, by Honeywell (1 mentions) Acetic acid, by Reanal (1 mentions) Gallic acid, by Merck Group (1 mentions) Trolox, by Merck Group (1 mentions) 2 4 6 tripyridyl s triazine tptz, by Merck Group (1 mentions) Folin ciocalteu reagent, by Merck Group (1 mentions) Petroleum ether, by Carlo Erba (1 mentions) Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions) Rnazol rt, by Merck Group (1 mentions) Lysozyme, by Merck Group (1 mentions) Direct zol rna miniprep plus kit, by Zymo Research (1 mentions) Trypan blue, by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Amino acid derivatives and resins, by Iris Biotech (1 mentions)

6 protocols using ethanol

1

Synthesis of Organosulfur Compounds

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Dimethyl trisulfide, sulfur, sodium sulfide, ammonium hydroxide, ethanol, and activated charcoal were purchased from Reanal, Hungary. Cysteine (168149), tetrabutyl ammonium iodide (140775), and allyl bromide (337528) were purchased from Sigma-Aldrich.
All reagents and materials were used without purification.
Nemes B., László S., Zsidó B.Z., Hetényi C., Feher A., Papp F., Varga Z., Szőke É., Sándor Z, & Pintér E. (2023). Elucidation of the binding mode of organic polysulfides on the human TRPA1 receptor. Frontiers in Physiology, 14, 1180896.
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2

Soxhlet Extraction and Polyphenol Determination

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Petroleum ether (Carlo Erba, Spain) boiling at 40–70 °C was used for Soxhlet extraction. Chemicals for the determination of polyphenol and antioxidant content were 97% ethanol (Reanal, Hungary), 99% methanol (Reanal, Hungary), anhydrous sodium carbonate (Riedel–de Haen, Germany), Folin–Ciocalteu reagent (Merck, Germany), 2-4-6-tripyridyl-s-triazine (TPTZ) (Sigma-Aldrich, USA), acetic acid (Reanal, Hungary), anhydrous iron chloride (Merck, Germany), 98% Trolox (Sigma-Aldrich, USA), gallic acid (Sigma-Aldrich, USA).
Kapcsándi V., Hanczné Lakatos E., Sik B., Linka L.Á, & Székelyhidi R. (2021). Antioxidant and polyphenol content of different Vitis vinifera seed cultivars and two facilities of production of a functional bakery product. Chemicke Zvesti, 75(11), 5711-5717.
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3

Bacterial RNA Purification Protocol

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For RNA purification, the frozen bacterial pellets were dissolved in 200 µL 10 mM Tris-EDTA (TE) buffer pH 8.0 containing lysozyme (3 mg/mL, Merck KGaA, Darmstadt, Germany), and incubated at 25 °C for 10 min. Then, 500 µL RNAzol RT (Merck KGaA, Darmstadt, Germany) was added, mixed by vortexing and incubated for 15 min at room temperature. After incubation, the mixture was centrifuged at 12,000× g for 15 min at 4 °C. An amount of 400 µL supernatant was removed and mixed with 400 µL ethanol (Reanal, Budapest, Hungary) in a new 1.5 mL tube. The RNA from the mix was further purified with a Direct-zol RNA MiniPrep Plus Kit (Zymo Research, Irvine, CA, USA), with DNase I treatment on the column. RNA was eluted with 50 µL DNase/RNase-free water and stored at −70 °C. The RNA quantities and the qualities were checked with a NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) and by a native 1% agarose gel.
Bozsó Z., Lapat V., Ott P.G, & Móricz Á.M. (2024). Disparate Effects of Two Clerodane Diterpenes of Giant Goldenrod (Solidago gigantea Ait.) on Bacillus spizizenii. International Journal of Molecular Sciences, 25(3), 1531.
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4

Synthesis and Evaluation of Fluorescent Compounds

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Amino acid derivatives and resins were obtained from Iris Biotech (Marktredwitz, Germany). Reagents, such as N,N′-diisopropylcarbodiimide (DIC), triisopropylsilane (TIS), 1-hydroxybenzotriazole (HOBt), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), Isoniazid (INH), glyoxylic acid and 5(6)-carboxyfluorescein (Cf) were purchased from Sigma (Budapest, Hungary). Trifluoroacetic acid (TFA) and acetonitrile (AcN) were from VWR (Budapest, Hungary). N,N-dimethylformamide (DMF), dichlormethane (DCM), diethyl ether, and ethanol were purchased from Reanal (Budapest, Hungary)
For the in vitro assays, RPMI-1640 medium, fetal calf serum (FCS), and trypan blue were obtained from Sigma (Budapest, Hungary), while DMEM medium and 2 mM l-glutamine were from Lonza (Basel, Switzerland). Trypsin, nonessential amino acids, and penicillin-streptomycin were from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). HPMI buffer (9 mM glucose, 10 mM NaHCO3, 119 mM NaCl, 9 mM HEPES, 5 mM KCl, 0.85 mM MgCl2, 0.053 mM CaCl2, 5 mM Na2HPO4 × 2H2O, pH = 7.4) was prepared in-house using components obtained from Sigma (Budapest, Hungary). BBL Löwenstein-Jensen medium was from Becton Dickinson (Környe, Hungary).
Horváti K., Fodor K., Pályi B., Henczkó J., Balka G., Gyulai G., Kiss É., Biri-Kovács B., Senoner Z, & Bősze S. (2021). Novel Assay Platform to Evaluate Intracellular Killing of Mycobacterium tuberculosis: In Vitro and In Vivo Validation. Frontiers in Immunology, 12, 750496.
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5

Analytical Methods for Bioactive Compounds

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Conventional distillation equipment was used to produce double distilled water for extraction and chromatography. Acetonitrile (LC-MS grade) was obtained from VWR-International (Budapest, Hungary). Potassium persulfate, 6-hydroxy-2,5,7,8tetramethylchroman-2-carboxylic acid (trolox), 2,2'-azino-bis(3-ethylbenzothiazoline-6sulphonic acid), sodium hydrogen phosphate, potassium dihydrogen phosphate, formic acid (98%), bovine serum albumin (92%), 3,3'-diaminobenzidine, polyvinylpyrrolidone (PVP-40), and Whatman GF/A glass fiber filter paper were procured from Sigma-Aldrich (Budapest, Hungary). Pyrocatechin, Coomassie Brilliant Blue G-250, ethanol, methanol, phosphoric acid, hydrogen peroxide were purchased from Reanal (Budapest, Hungary). Quercetin was obtained from Carl Roth GmbH (Karlsruhe, Germany).
Visi-Rajczi E., Hofmann T., Albert L., & Hansen J.K. (2021). Tracing the acclimation of European beech (Fagus sylvatica L.) populations to climatic stress by analyzing the antioxidant system. iForest - Biogeosciences and Forestry, 14(2), 95-103.
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6

Fluorescence-based Evaluation of ZEN-CD Complexes

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Fluorescence spectroscopic measurements were performed using a Hitachi F-4500 fluorimeter (Tokyo, Japan). Increasing amounts of CDs (final concentrations: 0, 25, 50, 100, 250, and 500 μM) were added to ZEN (2 μM), after which fluorescence emission spectra of ZEN and ZEN-CD complexes were recorded (λex = 315 nm). To approximate extracellular physiological conditions, experiments were carried out in phosphate-buffered saline (PBS, pH 7.4; containing 8.00 g/L NaCl, 0.20 g/L KCl, 1.81 g/L Na2HPO4 × 2H2O, and 0.24 g/L KH2PO4).
Stock solution of ZEN (5000 μM) was prepared in 96 v/v(%) ethanol (Reanal; Budapest, Hungary). In fluorescence spectroscopic studies, the concentration of ethanol did not exceed 0.04 v/v (%). Binding constants (K, unit: L/mol) of ZEN-CD complexes were determined employing the graphical application of the Benesi-Hildebrand equation, assuming 1:1 stoichiometry of complex formation (Poór et al., 2015b) :
where I0 and I are the fluorescence emission intensity of ZEN without and with CDs, respectively (λex = 315 nm, λem = 455 nm).
[CD] denotes the molar concentration of CDs (unit: mol/L), A is a constant, and n is the number of binding sites.
Faisal Z., Garai E., Csepregi R., Bakos K., Fliszár-Nyúl E., Szente L., Balázs A., Cserháti M., Kőszegi T., Urbányi B., Csenki Z, & Poór M. (2020). Protective effects of beta-cyclodextrins vs. zearalenone-induced toxicity in HeLa cells and Tg(vtg1:mCherry) zebrafish embryos. Chemosphere, 240.
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