Log In Sign up
The largest database of trusted experimental protocols

Axioimager m2 system

Manufactured by Zeiss
Visit Supplier
Sourced in United States

The AxioImager M2 system is a high-performance microscope platform designed for advanced imaging applications. It features a modular design, allowing for the integration of various components and accessories to meet the specific needs of scientific research and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Axiovision, by Zeiss (2 mentions) Apotome, by Zeiss (2 mentions) Ab227975, by Abcam (1 mentions) Alexa fluor 488 594 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions) Axiocam, by Zeiss (1 mentions) Vectashield fluorescence mounting medium, by Vector Laboratories (1 mentions) Lsm 700 confocal imaging system, by Zeiss (1 mentions) Gfp 1020, by Thermo Fisher Scientific (1 mentions) Volocity acquisition software, by PerkinElmer (1 mentions) Rnascope multiplex fluorescent assay kit, by Advanced Cell Diagnostics (1 mentions) Spinning disk confocal microscope system, by Zeiss (1 mentions)

Close spelling variants of this product

AxioImager M2 microscope system M2 AxioImager Axioimager system

10 protocols using axioimager m2 system

1

Immunohistochemical Analysis of T-cell Markers in IBM

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
We used Immunedeconv [31 (link)] in R along with CIBERSORT [32 (link)] (CIBERSORT, RRID:SCR_016955) to estimate the fraction of immune cells in our transcriptome dataset. CIBERSORT can characterize the heterogeneity of immune cells in bulk RNA-seq. This validated deconvolution approach can differentiate among 22 immune cell types including seven T-cell types, naïve and memory B cells, plasma cells, natural killer (NK) cells and myeloid subsets.
To show that increased LCK expression in IBM muscle compared to controls originates from T cells, we prepared 6 µm frozen sections of four IBM biopsies that were available for further experiments. The slides were immunostained overnight using primary antibodies anti-LCK (RabMab, clone EPR20798-107, Abcam ab227975, RRID: AB_2905531) and anti-CD3 (mouse mAb, clone OKT3, eBioscience™/Thermo Fisher Scientific, RRID:AB_467057). Alexa Fluor 488/594 -conjugated secondary antibodies (Thermo Fisher Scientific) were used for detection, and Hoechst was used for nuclear counterstaining. Microscopic images were obtained using the Zeiss Axio Imager M2 system (Carl Zeiss AG), with 40 × original magnification.
Johari M., Vihola A., Palmio J., Jokela M., Jonson P.H., Sarparanta J., Huovinen S., Savarese M., Hackman P, & Udd B. (2022). Comprehensive transcriptomic analysis shows disturbed calcium homeostasis and deregulation of T lymphocyte apoptosis in inclusion body myositis. Journal of Neurology, 269(8), 4161-4173.
+ Open protocol
+ Expand
2

Immunofluorescent Analysis of Muscle Biopsies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Snap-frozen muscle biopsies of patients III-6, IV-7, and IV-11 were used for the preparation of 8 µm sections for hematoxylin & eosin staining and immunofluorescent analysis using the standard methodology. Primary antibodies used were monoclonal mouse (mAb) anti-HNRNPA1 (Abcam ab5832), pAb anti-p62 (Sigma-Aldrich P0067), and mAb anti-TDP-43 (Sigma-Aldrich, clone 2E2-D3). Alexa fluor-488 and -546 conjugated secondary antirabbit and antimouse antibodies were used for detection, and the nuclei were counterstained using Hoechst. The slides were analyzed using the Zeiss Axio Imager M2 system using a 20× NA 0.80 objective (Carl Zeiss AG) or with a Leica TCS SP8 confocal microscope using a 63× NA 1.12 objective (Leica Microsystems).
Hackman P., Rusanen S.M., Johari M., Vihola A., Jonson P.H., Sarparanta J., Donner K., Lahermo P., Koivunen S., Luque H., Soininen M., Mahjneh I., Auranen M., Arumilli M., Savarese M, & Udd B. (2021). Dominant Distal Myopathy 3 (MPD3) Caused by a Deletion in the HNRNPA1 Gene. Neurology: Genetics, 7(6), e632.
+ Open protocol
+ Expand
3

Histological Analysis of Mouse Eyes

Check if the same lab product or an alternative is used in the 5 most similar protocols
The eyes were dissected from euthanized mice and fixed immediately in 4% PFA for overnight at 4°C, and then subjected to either paraffin embedding or OCT embedding for Cryostat sectioning. Seven μm of paraffin-embedded sections was cut and de-paraffinized prior to staining with hematoxylin and eosin (H&E). The mean thickness of the CNV membrane was obtained by examining the serial sections of whole eyes and using the maximum thickness measurements of each of the four lesion sites per eye. Frozen tissue sections from the Nestin-Cre+/−/mT/mG+/− and control mT/mG+/− mice were examined for the switch from red fluorescence to green fluorescence using Zeiss Axio Imager M2 system equipped with ApoTome, and attached with AxioCam and AxioVision cameras. The cell layers of the inner nuclear layer and outer nuclear layer were counted by random sampling 10 sagittal sections from each eye.
Lu H., Lu Q., Gaddipati S., Kasetti R.B., Wang W., Pasparakis M., Kaplan H.J, & Li Q. (2014). IKK2 Inhibition Attenuates Laser-Induced Choroidal Neovascularization. PLoS ONE, 9(1), e87530.
+ Open protocol
+ Expand
4

Quantifying Lens Epithelial Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were injected with BrdU intraperitoneally (150 mg/kg body weight), and sacrificed two hours later. Eyes were enucleated and fixed in 4% paraformaldehyde at 4°C for 24 hours. Whole lenses were dissected and the antigen retrieval procedures followed as described above. Anti-BrdU primary antibody was used to detect the BrdU incorporated cells, and visualized by fluorescent-dye conjugated secondary antibodies. Nuclei were stained by 4′,6-diamido-2-phenylindole (DAPI). Lens capsules were peeled off and placed on glass slides with the epithelium facing up, and mounted in Vectashield fluorescence mounting medium (Vector Labs, Burlingame, CA, USA). The fluorescent images were examined and photographed using a Zeiss Axio Imager M2 system with ApoTome, AxioCam, and AxioVision (Zeiss, Peabody, MA, USA). The number and percentage of BrdU-positive cells at the germinative zone were counted in lens epithelial capsule explants from three mice in each experimental group, and at least three images were used for counting the BrdU-positive cells for each mouse. All data were summarized as the mean ± SD. A two-tailed Student t-test was performed to determine the significance of the differences between means. The differences were considered statistically significant if the P values were less than 0.05.
Lu Q., Zhang Y., Kasetti R.B., Gaddipati S., CVM N.K., Borchman D, & Li Q. (2020). Heterozygous Loss of Yap1 in Mice Causes Progressive Cataracts. Investigative Ophthalmology & Visual Science, 61(12), 21.
+ Open protocol
+ Expand
5

Quantifying C. elegans Mechanosensory Neuron Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals with zdIs5(Pmec-4::GFP) or jsIs973(Pmec-7::RFP) were anesthetized by 1% sodium azide, and the PLMs were imaged under the 10X objective of an AxioImager M2 system (Carl Zeiss). The length of the PLM process and the distance between the PLM branch and the cell soma were analyzed using ImageJ [55 (link)]. Each animal was only scored for left or right PLM based on which side is clearly visible in the image. There was no side-to-side difference in PLM branch positions, so data from left and right PLMs were pooled for analyses. We define normal PLM branch locations as those that fall within 99% (mean ± 3 standard deviations) of PLM branch sites visualized by zdIs5(Pmec-4::GFP) in the wild type, which ranges from 0.46 to 0.88. The percentage of both proximally and distally mislocalized PLM branches in indicated genotypes was calculated and presented along with the scatter plots.
Chen C.H., He C.W., Liao C.P, & Pan C.L. (2017). A Wnt-planar polarity pathway instructs neurite branching by restricting F-actin assembly through endosomal signaling. PLoS Genetics, 13(4), e1006720.
+ Open protocol
+ Expand
6

Quantifying Mitochondrial Stress in C. elegans

Check if the same lab product or an alternative is used in the 5 most similar protocols
Synchronized L1 animals with the integrated transgene zcIs13(Phsp-6::GFP) were fed with indicated RNAi for 48 hours. GFP images were acquired under the 10X objective of the AxioImager M2 system (Carl Zeiss). Each image includes 10 animals, and three images were quantified for one individual experiment. GFP intensity was quantified by ImageJ and normalized to that of the vector control RNAi.
Liao C.P., Chiang Y.C., Tam W.H., Chen Y.J., Chou S.H, & Pan C.L. (2022). Neurophysiological basis of stress-induced aversive memory in the nematode Caenorhabditis elegans. Current biology : CB, 32(24).
+ Open protocol
+ Expand
7

Quantifying Neuronal and Mitochondrial Morphology

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze the expression level of Ptph-1::mCherry in the cell body of serotonergic neurons, D1 hermaphrodites were immobilized in 1% sodium azide on 5% agar pad and epifluorescent images were acquired by the AxioImager M2 system (Carl Zeiss). The pixel intensity of mCherry was quantified by ImageJ with subtraction of background signals. To examine the mitochondrial morphology in the intestine and body wall muscles, D1 animals carrying zcIs14(Pmyo-3::GFPmt) and animals carrying zcIs17(Pges-1:: GFPmt) were treated with ethanol (control) or 4.5 μM Antimcyin A for 6 h. To examine mitochondrial morphology in NSM neurons, D1 animals carrying twnEx609(Ptph-1s::tomm20::mCherry); twnEx605(Ptph-1s::ds-atp-2) were immobilized with 1 mM levamisole. Confocal projection images were acquired using the LSM700 Confocal Imaging System (Carl Zeiss).
Chiang Y.C., Liao C.P, & Pan C.L. (2022). A serotonergic circuit regulates aversive associative learning under mitochondrial stress in C. elegans. Proceedings of the National Academy of Sciences of the United States of America, 119(11), e2115533119.
+ Open protocol
+ Expand
8

Quantifying NFIX+ and GFP+ Cells in BNSTp

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Brains were dissected from perfused P14 Esr1Cre/+;Sun1–GFPlx/+ animals and cryosectioned at 40 μm before immunostaining with primary antibodies to GFP (1:1,000, Aves GFP-1020) and Nfix (1:1,000, Thermo Fisher PA5-30897), and secondary antibodies against chicken (1:300, Jackson Immuno 703-545-155) and rabbit (1:800, Jackson Immuno 711-165-152), as previously described16 (link). A Zeiss Axioimager M2 System equipped with MBF Neurolucida Software was used to take 20× wide-field image stacks spanning the BNSTp (five sections, both sides). The number of Nfix+, GFP+ and Nfix+GFP+ cells was quantified using Fiji/ImageJ from the centre three optical slices by an investigator blinded to condition.
Gegenhuber B., Wu M.V., Bronstein R, & Tollkuhn J. (2022). Gene regulation by gonadal hormone receptors underlies brain sex differences. Nature, 606(7912), 153-159.
+ Open protocol
+ Expand
9

Immunostaining of Fixed Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostaining was performed on fixed cryosections, paraffin sections, or fixed cells as described (Johnston et al., 2013) with some modifications. Digital image acquisition was performed with a Quorum spinning disc confocal microscope using Volocity acquisition software (PerkinElmer) or on a Zeiss AxioImager M2 system. Further details and antibodies are in Supplemental Experimental Procedures.
Johnston A.P., Yuzwa S.A., Carr M.J., Mahmud N., Storer M.A., Krause M.P., Jones K., Paul S., Kaplan D.R, & Miller F.D. (2016). Dedifferentiated Schwann Cell Precursors Secreting Paracrine Factors Are Required for Regeneration of the Mammalian Digit Tip. Cell stem cell, 19(4).
+ Open protocol
+ Expand
10

Immunostaining and FISH Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostaining was performed essentially as described (Gallagher et al., 2013) . FISH was performed using the RNAScope Multiplex Fluorescent Assay kit (Advanced Cell Diagnostics). Images of immunostaining were collected using a Quorom spinning-disk confocal microscope system or a Zeiss Axio Imager M2 system with an X-Cite 120 LED light source and a C11440 Hamamatsu camera. For FISH, z stacks of confocal images were taken with optical slice thickness of 0.2 mm, and projected z stacked images are shown. Further details, FISH probes and antibodies are in Supplemental Experimental Procedures.
Yuzwa S.A., Yang G., Borrett M.J., Clarke G., Cancino G.I., Zahr S.K., Zandstra P.W., Kaplan D.R, & Miller F.D. (2016). Proneurogenic Ligands Defined by Modeling Developing Cortex Growth Factor Communication Networks. Neuron, 91(5).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!