Rm 2145 rotary microtome

Manufactured by Leica

Sourced in Germany

The Leica RM 2145 rotary microtome is a laboratory instrument designed for the precise sectioning of specimens for microscopic examination. It features a reliable and durable mechanism that allows for the controlled and consistent cutting of thin tissue sections.

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Lab products found in correlation

Paraplast, by Leica (2 mentions) Permount, by Thermo Fisher Scientific (2 mentions) Dmlb light microscope, by Leica (2 mentions) Sigma vp scanning electron microscope, by Zeiss (2 mentions) Jsm 5900lv, by JEOL (1 mentions) Quanta 200 scanning electron microscope, by Thermo Fisher Scientific (1 mentions) Edwards e 1010 ion sputter golden coater, by Hitachi (1 mentions) S6d stereomicroscope, by Leica (1 mentions) K850 critical point dryer, by Emitech (1 mentions) Picro sirius red stain kit, by Abcam (1 mentions) Paraplast, by Thermo Fisher Scientific (1 mentions) Permount resin, by Thermo Fisher Scientific (1 mentions) Axio imager m2 microscope, by Zeiss (1 mentions) Toluidine blue o, by Merck Group (1 mentions) Entellan, by Merck Group (1 mentions) Technovit 7100 resin, by Kulzer (1 mentions)

Spelling variants of this product

Microtome (1520 citations) Rotary microtome (327 citations) Rotary Microtome 2145 (2 citations)

9 protocols using rm 2145 rotary microtome

Micromorphology and Anatomy of Plant Nectaries

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Shoot apices and young leaves were fixed in formalin–acetic acid–alcohol (FAA) solution for 24 h [62 ] and buffered neutral formalin in 0.1 M sodium phosphate buffer (pH 7.0) for 48 h [63 ]. For the micromorphological study, mature nectaries were isolated, dehydrated in a graded ethanol series, dried by the critical point method, mounted on aluminium stub, and sputter-coated with gold, with subsequent observation in a Zeiss Sigma VP scanning electron microscope (Carl Zeiss, Oberkochen, Germany).
For anatomical analyses, shoot apices and young leaves were isolated, dehydrated through a tertiary butyl alcohol series [62 ], embedded in Paraplast^® (Leica Microsystems Inc., Heidelberg, Germany), and serial-sectioned at a 12 µm thickness on a Leica RM2145 rotary microtome. Longitudinal and transverse sections were stained with astra blue and safranin O [64 ] and the slides were mounted with resin Permount (Fisher Scientific, Pittsburgh, PA, USA). Observations and photographs were performed using a Leica DMLB light microscope.

Maximo D., Ferreira M.J, & Demarco D. (2023). Inaugural Description of Extrafloral Nectaries in Sapindaceae: Structure, Diversity and Nectar Composition. Plants, 12(19), 3411.

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Paraffin Embedding for Sonchus arvensis L. Callus Anatomy

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This method was used to determine the anatomical structure of Sonchus arvensis L. callus. The callus at 21 days was used. The technique was the paraffin cloaking method (paraffin embedding). The samples were fixed in standard fixative for paraffin embedding (70% alcohol). Fixed materials were dehydrated serially using alcohol and water, which replaces the water in the material. A paraffin wax of melting point 58–60°C was used for infiltration and embedding. Uniformly thin sections of 10 μm thickness were cut using Leica RM 2145 rotary microtome. Safranin stain was used to localize polysaccharides of the cell wall.

Wahyuni D.K., Rahayu S., Zaidan A.H., Ekasari W., Prasongsuk S, & Purnobasuki H. (2021). Growth, secondary metabolite production, and in vitro antiplasmodial activity of Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment. PLoS ONE, 16(8), e0254804.

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Micromorphology and Anatomy of Plant Nectaries

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Maximo D., Ferreira M.J, & Demarco D. (2023). Inaugural Description of Extrafloral Nectaries in Sapindaceae: Structure, Diversity and Nectar Composition. Plants, 12(19), 3411.

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Histochemical Analysis of GUS Expression in Transgenic Seeds

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Seeds (harvested from transgenic plants grown in the greenhouse) at different stages of development (S1, S2, S3, and S5) were cut transversally and subjected to histochemical staining for GUS activity as described by Jefferson et al. (1987) (link) and modified (Kosugi et al., 1990 (link)) to avoid background that could be due to non-specific endogenous GUS activity (i.e., with 20% MeOH in 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid (X-Gluc) solution). A K⁺ ferricyanide/ferrocyanide mixture (2 mM of each) was also added to the incubation buffer to prevent the diffusion of the indoxyl derivative before its oxidative dimerization.
Semi-thin sections were then obtained as described by Hawkins et al. (2002) . Briefly, GUS-stained samples were fixed in formalin acetic alcohol (FAA) [10% formalin (37% formaldehyde stabilized with MeOH), 5% glacial acetic acid, 60% EtOH] for 24 h, dehydrated and embedded in paraffin. Semi-thin sections (5 μm) were made on a RM 2145 rotary microtome (Leica, Wetzlar, Germany), then deparaffinized and counter-stained with periodic acid Schiff to visualize the cell walls in each tissue before permanent mounting and microscopic observation. Pictures were taken with a Nikon Coolpix 5400 digital camera (Nikon, Tokyo, Japan). The same experiments were conducted on different stage wild type seeds as negative control.

Fang J., Ramsay A., Renouard S., Hano C., Lamblin F., Chabbert B., Mesnard F, & Schneider B. (2016). Laser Microdissection and Spatiotemporal Pinoresinol-Lariciresinol Reductase Gene Expression Assign the Cell Layer-Specific Accumulation of Secoisolariciresinol Diglucoside in Flaxseed Coats. Frontiers in Plant Science, 7, 1743.

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Morphological Analysis of Theraphosidae and Nemesiidae

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An area comprising the proximal part of the abdomen next to the genital opening was detached from the specimens andcleaned with 80% ethanol. Eachsample wasproceededto critical point dry ing (POLAROÑ E3000), using Carbon Dioxide (CO2) as transitional fluid. Sample structure wasmountedbyplacingitonapiece ofdouble sided sticky carbon tape on an aluminum stub. The last step of preparationbeforetakingtheSEM images involvedsputtering coat-ingbyusinggoldinArgonwithplasmacurrent(SPIModuleTM). The examinations were made undervariable pressure conditions using a JEOL JSM-35CF (JEOL Ltd., Japan) and a JSM-5900LV (JEOL, USA Inc., USA) scanning electron microscopes. The microscopes were operated at high vacuum.
For histological approach, the abdomen of one adult male of Grammostola vachoni (Theraphosidae) and an adult male of Acanthogonatus centralis (Ñemesiidae) were fixed in Dubosq-Brasil solution and embedded in Paraplast®. Tissue sections of 5 ^m thickness were cut using a Leica RM 2145 rotary microtome, and stained withhematoxylin-eosin andMasson's trichromicprotocols for morphologic analysis. The periodic acid Schiff (PAS) reaction and the Alcian blue (AB) technique at 2.5 pH level were employed to analyze the glycoprotein distribution. The slides were observed with a Ñikon AFM microscope and were digitalized with an Olym pus Camedia Mod.C-7070 Wide Zoom.

Ferretti N., Pompozzi G., Copperi S., Wehitt A., Galíndez E., González A, & Pérez-Miles F. (2017). A comparative morphological study of the epiandrous apparatus in mygalomorph spiders (Araneae, Mygalomorphae). Micron (Oxford, England : 1993), 93.

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Floral Bud Development Analysis

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The stamen primordia and gynoecium primordia were dissected carefully and photographed using a LEICA S6D stereomicroscope (Leica Microsystems, Wetzlar, Germany). Then, the floral buds, stamen primordia, and gynoecium primordia were fixed in FAA for 24 h for SEM and paraffin sectioning. Some fixed materials were dehydrated in an ethanol series for 20 min per step and dried using CO₂ as the exchange agent by an EMITECH K850 critical point dryer (Emitech, Canterbury, British). Then, the samples were coated with gold by an Edwards E-1010 ion sputter golden coater (Hitachi, Tokyo, Japan) and photographed with an FEI Quanta 200 scanning electron microscope (FEI, Eindhoven, Netherlands).
The remaining fixed materials were dehydrated in an ethanol series, transparentized in a xylene series, infiltrated in paraffin, embedded in paraffin wax, and sectioned at an 8 µm thickness with a LEICA RM2145 rotary microtome (Leica Microsystems, Wetzlar, Germany). Finally, the sections were stained with safranin and fast green and photographed with a Zeiss AXIO Axioscope A1 fluorescence microscope (Carl Zeiss, Jena, Germany).

Liu H., Yang L., Tu Z., Zhu S., Zhang C, & Li H. (2021). Genome-wide identification of MIKC-type genes related to stamen and gynoecium development in Liriodendron. Scientific Reports, 11, 6585.

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Collagen Maturation Analysis in Healing Skin

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Tissue samples of healing skin were fixed in 10% buffered formalin, dehydrated by a gradual alcohol series, cleared in xylene, embedded in paraffin blocks and sectioned at 8 µm using a Leica RM2145 rotary microtome (Leica Microsystems, Wetzlar, Germany). After deparaffinization, sections were stained for collagen fibers using a Picro Sirius red stain kit (Abcam, London, UK; ab150681). The standard protocol recommended by the manufacturer was used, including using muscle samples as negative controls. The semi-quantitative analysis of total collagenous tissue maturation was carried out according to Maia-Figueiro et al. [66 ] with a slight modification; the experimental samples were classified in the following three grades of red intensity in comparison with the red intensity of the control sample: “0” (equal intensity—same maturation); “−” (lower intensity—less advanced maturation); “+” (higher intensity—more advanced maturation).

Komprda T., Sládek Z., Vícenová M., Simonová J., Franke G., Lipový B., Matejovičová M., Kacvinská K., Sabliov C., Astete C.E., Levá L., Popelková V., Bátik A, & Vojtová L. (2022). Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model. International Journal of Molecular Sciences, 23(14), 7663.

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Floral Developmental Stages Analysis

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Inflorescences containing flowers at several developmental stages were collected at the Instituto de Biociências of Universidade de São Paulo and at the Instituto de Botânica in São Paulo. Vouchers of the species were provided and the vouchers were deposited in the herbarium of the Universidade de São Paulo (SPF). The material was fixed in formalin, acetic acid, 50% ethyl alcohol (FAA) for 24 h [16 ] or by buffered neutral formalin (BNF) for 48 h [17 ], and then stored in 70% ethyl alcohol.
Inflorescence meristems, flower buds, and anthetic and post-anthetic flowers were isolated, dehydrated in a butyl series [16 ], embedded in Paraplast (Fisher Healthcare, Houston, Texas, USA), and transversely and longitudinally sectioned using a Leica RM2145 rotary microtome (Leica Microsystems, Wetzlar, Germany). Serial sections around 12 μm thick were stained with astra blue and safranin [18 ], and mounted in Permount resin (Fisher Scientific, Pittsburgh, Pennsylvania, USA). Samples were observed and photographed using a Leica DMBL light microscope (Leica Microsystems, Wetzlar, Germany).

Gagliardi K.B., Cordeiro I, & Demarco D. (2018). Structure and development of flowers and inflorescences in Peraceae and Euphorbiaceae and the evolution of pseudanthia in Malpighiales. PLoS ONE, 13(10), e0203954.

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Histological Analysis of Regenerative Tissue

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For histological examination, samples of tissue clumps collected from the solid medium 4 weeks after induction of regeneration, were fixed in freshly prepared 100 mM phosphate buffer (pH 7.2) containing 2% (v/v) formaldehyde (POCH) and 3% (v/v) glutaraldehyde (POCH) for at least 48 h at room temperature (with vacuum infiltration within the first 10 min). After fixation, the tissue samples were dehydrated in a graded ethanol series (10%, 30%, 50%, 70%, and 90% for 15 min each) and left overnight in absolute ethanol. The dehydrated material was embedded in Technovit ® 7100 resin (Kulzer, Germany), according to the manufacturer's protocol. When polymerized, the samples were sectioned into slices of 4 μm thickness using a Leica RM2145 rotary microtome (Leica Microsystems GmbH, Germany) with a Leica TC-65 carbide blade. The sections were then stained with 1% (w/v) toluidine blue O (Sigma-Aldrich), permanently mounted in Entellan ® (Merck, Germany), and examined under an Axio Imager.M2 microscope (Carl Zeiss, Germany).

Klimek‐Chodacka M., Kadłuczka D., Łukasiewicz A., Malec-Pala A., Barański R., & Grzebelus E. (2020). Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L. Plant Cell Tissue and Organ Culture (PCTOC), 143(3), 693-707.

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