Shimadzu LC-2030C 3D HPLC System (Shimadzu, Tokyo, Japan), Ultrasonic Water Baths (Grant, Cambridge, UK), Memmert Waterbath WNB 14 (Memmert, Bayern, Germany), Hettich EBA20S Portable Centrifuge (Hettich, Tuttlingen, Germany), HPLC Column Phenomenex C18 Prodigy™ ODS-3 100 Å (150 mm × 4.6 mm, 5 µm) (Phenomenex, CA, USA), and HPLC Column Phenomenex C18 Prodigy™ ODS-3 100 Å (250 mm × 4.6 mm, 5 µm) (Phenomenex, CA, USA).
Eba20s portable centrifuge
Manufactured by Hettich
Sourced in Germany, United Kingdom
The EBA20S Portable Centrifuge is a compact and lightweight laboratory equipment designed for general-purpose centrifugation. It features a fixed-angle rotor and can accommodate a range of sample tube sizes. The centrifuge is suitable for a variety of applications that require the separation of components within a liquid sample.
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3 protocols using eba20s portable centrifuge
1
HPLC Analysis of Pharmaceutical Compounds
2
Quantitative Analysis of Metabolites
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Ultrasonic water baths (Grant, United Kingdom), Water Bath WNB 14 (Memmert, Germany), Vortex mixer (Stuart, United Kingdom), EBA20S Portable Centrifuge (Hettich, Germany), Analytical balance (Ohaus, United States), HPLC Column Phenomenex C18 Prodigy™ ODS-3100 Å (150 mm × 4.6 mm, 5 μm) (Phenomenex, United States), pH meter (Mettler Toledo, Switzerland), Oven (Memmert, Germany), Shimadzu LC-2030C 3D HPLC System (Shimadzu, Tokyo, Japan), Thermo Scientific Genesys 10S UV–Vis spectrometer (Thermo Scientific, United States), Whitley DG250 Anaerobic Workstations (Whitley, England).
3
Green Synthesis of Silver Nanoparticles
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Silver nitrate was used as precursor for synthesis of silver nanoparticles. 5 ml of 1 mM silver nitrate aqueous solution was added to each 100 ml of clear plant extract. The flasks were put into shaker (150 rpm) at 30 °C and reaction was carried out for a period of 72 h. In this process, A. cepa (onion) and A. sativa (garlic) extracts act as the reducing and stabilizing agents. Silver nanoparticles were obtained gradually by the erosion and chemical degradation of plant extract. The brown colors formation indicate that the AgNPs were synthesized from the plant extracts and they were centrifuged at 5000 rpm (Hettich EBA20S Portable Centrifuge) for 10 min in order to obtain the pellet which is used for further study.
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