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2 protocols using anti mbd2 3

1

Comprehensive Chromatin Regulatory Network

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The antibodies used were: anti-SIX3, anti-H3K4me1, anti-H3K4me2, anti-JAG1, anti-GLI1, anti-ZEB2, anti-ANGPTL4 (Abcam, Hong Kong, China), anti-NCOA3 (BD Biosciences, USA), anti-MTA3, anti-MBD2/3, anti-H3pan-ac (Millipore, Billerica, MD, USA), anti-LSD1, anti-HDAC1, anti-HDAC2, anti-RbAp46/48 (Sigma-Aldrich, St Louis, MO, USA), anti-MTA1, anti-MTA2, and anti-WNT1 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Human recombinant Wnt1 was purchased from Sigma-Aldrich. Protein A/G Sepharose CL-4B beads were from Amersham Biosciences (Indianapolis, IN, USA) and shRNAs were from GenePharma Co., Ltd (Shanghai, China).
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2

Comprehensive Chromatin Remodeling Antibody Panel

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The sources of the antibodies were anti-FLAG, anti-HDAC1 anti-HDAC2, anti-RbAp46/48, anti-Fibronectin, anti-Vimentin and anti-β-actin (Sigma–Aldrich); anti-DDB1, anti-MTA1, anti-SIN3A, anti-SAP180 and anti-SAP30 (Santa Cruz Biotechnology); anti-PRMT5, anti-ROC1, anti-LSD1, anti-DNMT3B, anti-HDAC5 and anti-MTA2 (Abcam); anti-EED, anti-MBD2/3 and anti-MTA3 (Millipore); anti-SUZ12 (Cell Signaling Technology); anti-WDR77 (also known as MEP50, Bethyl); anti-MTA2, anti-E-cadherin, anti-α-catenin, anti-γ-catenin, anti-N-cadherin and anti-EZH2 (BD Bioscience); anti-ING4 (Genetex), anti-SETD2 (Proteintech). Dynabeads Protein G was obtained from Invitrogen by Thermo Fisher Scientific, and protease inhibitor mixture cocktail was from Roche Applied Science. Glutathione-Sepharose 4B beads were from GE Healthcare Bio-Sciences. Short hairpin RNAs (shRNAs) were obtained from GenePharma Co Ltd (Shanghai, China).
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