5 and 6 carboxyfluorescein diacetate succinimidyl ester

Manufactured by Thermo Fisher Scientific

5-(and 6)-carboxyfluorescein diacetate succinimidyl ester is a fluorescent labeling reagent. It is commonly used for covalent labeling of proteins and peptides.

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Lab products found in correlation

Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Fv1000 microscope, by Olympus (1 mentions)

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Carboxyfluorescein diacetate succinimidyl ester (44 citations) 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (23 citations) 5,6-carboxyfluorescein diacetate succinimidyl ester (9 citations) 5-(and-6)-carboxyfluorescein (3 citations) 5(6)-Carboxyfluorescein (2 citations)

2 protocols using 5 and 6 carboxyfluorescein diacetate succinimidyl ester

Cell Proliferation Assay with CFSE

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Cells were resuspended in PBS at 5–10 × 10⁶/mL and incubated with 10 μmol/L 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (Ebioscience, San Diego, CA) for 10 minutes at room temperature in the dark. The cells were then washed twice with complete medium and transferred to 6-well co-culture plates (pore size, 4 μm; Corning, Corning, NY). After co-culture for 48 hours, 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester–labeled cells were collected and subjected to flow cytometry using a 488 nm excitation source.

Fan M., Shi H., Yao H., Wang W., Zhang Y., Jiang C, & Lin R. (2022). BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury. Cellular and Molecular Gastroenterology and Hepatology, 15(2), 511-531.

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Membrane Colocalization Study Protocol

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To conduct the membrane colocalization study, RBC membrane was labeled with 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD; excitation/emission = 644/663 nm; Biotium) and platelet membrane with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, excitation/emission = 494/521 nm; eBiosciences). RBCNPs, PNPs, or [RBC-P]NPs made using these labeled membranes were then visualized under confocal fluorescence microscopy using an Olympus FV1000 microscope with a 100× oil objective. In order to obtain stable images, the particles were dispersed in glycerol to significantly decrease their mobility.

Dehaini D., Wei X., Fang R.H., Masson S., Angsantikul P., Luk B.T., Zhang Y., Ying M., Jiang Y., Kroll A.V., Gao W, & Zhang L. (2017). Erythrocyte-Platelet Hybrid Membrane Coating for Enhanced Nanoparticle Functionalization. Advanced materials (Deerfield Beach, Fla.), 29(16), 10.1002/adma.201606209.

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