Breast cancer cell lines MCF7, T47D, HCC1954, MDA-MB-453 and SK-BR-3 were purchased from ATCC and SUM149 was purchased from Asterland. MCF7, T47D, HCC1954 and SK-BR-3 were cultured in RPMI medium 1640 (Gibco, USA) with 10% FBS (Gibco, USA). MDA-MB-453 was cultured in Leibovitz’s L-15 medium (Sigma, USA) supplemented with 10% FBS. SUM149 was cultured in Ham’s F-12 medium (Invitrogen, USA) supplemented with 5% FBS, 5 μg/mL of insulin (Beyotime, China) and 1 ug/mL of hydrocortisone (Sigma, USA). MCF7, T47D, HCC1954, SUM149 and SK-BR-3 cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 and MDA-MB-453 was cultured at 37 °C with 100% humidified atmosphere. The cell lines were profiled routinely by short tandem repeat analysis.
Hcc1954
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HCC1954 is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed for specific scientific applications, but a detailed functional description cannot be provided while maintaining an unbiased and factual approach. More information from the manufacturer would be required to give an accurate and comprehensive overview of the product's core function.
Automatically generated - may contain errors
Lab products found in correlation
Hcc1954, by American Type Culture Collection (9 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (9 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (8 mentions)
Mcf 7, by American Type Culture Collection (7 mentions)
Mcf 7, by Thermo Fisher Scientific (6 mentions)
Rpmi 1640, by Thermo Fisher Scientific (5 mentions)
Sk br 3, by Thermo Fisher Scientific (4 mentions)
Skbr3, by American Type Culture Collection (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Mda mb 453, by American Type Culture Collection (3 mentions)
Mda mb 231, by Thermo Fisher Scientific (3 mentions)
Bt474, by Thermo Fisher Scientific (3 mentions)
Hydrocortisone, by Merck Group (2 mentions)
1640 medium, by Thermo Fisher Scientific (2 mentions)
Hcc38, by Thermo Fisher Scientific (2 mentions)
Mcf 10a, by Lonza (2 mentions)
Bt474, by American Type Culture Collection (2 mentions)
Mda mb 453, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Mcf 10a, by American Type Culture Collection (2 mentions)
15 protocols using hcc1954
1
Culturing and Characterizing Breast Cancer Cell Lines
2
Culturing Diverse Breast Cell Lines
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Human normal breast epithelial cells (MCF-10A) and BRCA cell lines (MCF-7, MDA-MB-231, MDA-MB-468, HCC1954, HCC38, SK-BR3, and T47D) were obtained from American Typical Culture Collection (ATCC, Manassas, VA). Normal breast MCF-10A cells were cultured in a mammary epithelium-specific medium (Lonza). MDA-MB-231 and MDA-MB-468 cells were cultured in sealed medium containing L-15 basal medium (Solarbio). MCF-7 cells were cultured in DMEM medium (Gibco). HCC1954, HCC38, SK-BR3, and T47D cells were cultured in 1640 medium (Gibco). In addition to the above dedicated medium, the addition of 10% fetal bovine serum (VivaCell) was essential, while penicillin/streptomycin (Hyclone) was selectively added according to the cell status. Growth conditions for all cells were 37°C and 5% CO2 concentration.
3
LXR Agonist and Inhibitor Screening
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Novel LXR ligand GAC0001E5 (1E5) was synthesized by Otavachemicals (Concord, ON, Canada). LXR agonist GW3965 was received from Cayman Chemicals (Ann Arbor, MI, USA #10054). Tyrosine kinase inhibitor lapatinib and fatty acid synthase inhibitor C75 were purchased from MedChemExpress [South Brunswick Township, NJ, USA (lapatinib #HY-50898, C75 #HY-12364)]. AU565, HCC-1954, and SKBR3 cell lines were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). AU565 and HCC-1954 cells were cultured in RPMI 1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA #11875085). SKBR3 cells were cultured in DMEM (Gibco, #12430047) containing high glucose and HEPES. All media were supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, #26140079). All cell cultures were maintained in a humidified incubator of 5% CO2 at 37 °C.
4
Cell culture of breast cancer lines
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HCC1954, MDA-MB-453, SkBr3 and BT474 cells were from ATCC-LGC Standards (Teddington, UK). SkBr3 and BT474 were maintained in Dulbecco's Modified Eagle's Media (DMEM):F-12, HCC1954 in RPMI 1640 and MDA-MB-453 in Leibovitz’s L-15, all 10% FBS and 4 mmol/L L-glutamine (all from Gibco).
5
KNSTRN Gene Manipulation in Breast Cell Lines
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The cell lines, including MDA-MB-231, HS578T, UACC812, MCF7, T47D, HCC1954, and MCF10A, were obtained from the Shanghai Institute of Biological Sciences and the Chinese Academy of Sciences. MDA-MB-231, HS578T, UACC812, MCF7, and T47D cells were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum (Gibco). HCC1954 cells were cultured in RPMI1640 (Gibco) supplemented with 10% fetal bovine serum. The normal breast epithelial cell line MCF10A was cultured in mammary epithelial cell basal medium (Lonza). All cells were maintained at 37 °C under a 5% CO2 atmosphere. The siRNAs used in this study were purchased from GenePharma (Suzhou). According to the manufacturer's instructions, Plasmid and siRNA transfection experiments were performed using Lipofectamine 3000 (Invitrogen). The KNSTRN gene was cloned into a TK-PCDH-copGFP-T2A-Puro vector between Nhel and Notl restriction sites. The siRNAs targeting KNSTRN had the following sequences: siRNA-KNSTRN#1: 5ʹ-GCU ACA AAC CAC UGA GUA ATT-3ʹ and siRNA-KNSTRN#2: 5ʹ-CCG AUU CCU AGA ACA GCA ATT-3ʹ.
6
Breast Cancer Cell Lines and Culture Conditions
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The breast cancer cell lines MCF-7, MDA-MB-231, HCC1954 and the non-transformed mammary epithelial MCF-10A cells were obtained from the ATCC (Manassas, VA, USA). MCF-7 cells were cultured in DMEM (Invitrogen, Carlsbad, CA) supplemented with 1% PS (penicillin and streptomycin) and 10% fetal bovine serum (FBS). MDA-MB-231 cells and HCC1954 cells were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 1% PS and 10% FBS. MCF-10A cells were cultured in Dulbecco’s modified Eagle's medium supplemented with 1% PS and 10% FBS. Cells were grown at 37°C in the presence of 5% CO2.
7
Breast Cancer Cell Line Cultivation and Validation
8
Breast Cancer Cell Line Characterization
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All cell lines were purchased from the American Type Culture Collection (ATCC). The following Her2− human breast cancer cell lines were used: HCC38, HCC1395, HCC70, MDA231, MDA468, HCC1806, HCC1143 and HCC1937. The following HER2+ breast cancer cell lines were used: HCC202, HCC1569, HCC1419, BT474, UACC893, CRL2351 and HCC1954. UACC893, MDA468 and BT474 cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS) (Hyclone), and 1% penicillin/streptomycin (P/S). All other cancer cell lines (HCC38, HCC1937, HCC1395, HCC70, HCC1806, HCC1143, HCC202, HCC1569, HCC1419, CRL2351, HCC1954 and MDA231) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen), supplemented with 10% FBS and 1% P/S. MCF10A cells were grown in DMEM/F12 medium supplemented with 5% horse serum, 10 μg/ml insulin, 20 ng/ml EGF, 0.5 μg/ml hydrocortisone, 100 ng/ml Cholera toxin and 1% P/S. Cells were grown at 37°C in 5% CO2. Retroviral and lentiviral infections were performed as previously described [35 (link)].
9
Cell Line Characterization and Maintenance
10
Culturing Diverse Breast Cell Lines
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The breast cell lines MCF-10A (ATCC® CRL10317™), MCF-7 (ATCC® HTB22™), and HCC-1954 (ATCC® CRL2338™) were purchased from the American Type Culture Collection (ATCC®, Rockville, MD, USA). MDA-MB-231 and ZR-75-30 cell lines were donated by Nadia Jacobo Herrera from Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán” in Mexico City, Mexico. Non-tumorigenic human breast cells (MCF-10A) were grown in Mammary Epithelial Basal Medium® supplemented with SingleQuots® from the MEGM® BulletKit® (Lonza, Walkersville, MD, USA). The tumorigenic breast cell lines, ZR-75-30 and HCC-1954, were grown in Gibco® Roswell Park Memorial Institute (RPMI)-1640 medium (Thermo Fisher Scientific, Grand Island, NY, USA) supplemented with 10% and 15% heat-inactivated Gibco® Fetal Bovine Serum (FBS) (Thermo Fisher Scientific), respectively. The MCF-7 and MDA-MB-231 cell lines were grown in Gibco® Dulbecco’s Modified Eagle Medium, which features a nutrient mixture of D-MEM and F-12 (Thermo Fisher Scientific), supplemented with 10% heat-inactivated FBS. Each culture medium was supplemented with Gibco® Penicillin-Streptomycin antibiotics (Thermo Fisher Scientific). Cell cultures were grown at 37 °C with 5% CO2 and 95% air in a humidified incubator.
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