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Fluorescein mal 1

Manufactured by Vector Laboratories
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Fluorescein-MAL-1 is a fluorescent labeling reagent. It is used to covalently attach fluorescein to proteins or other biomolecules containing free sulfhydryl groups.

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Lab products found in correlation

Fluorescein sna, by Vector Laboratories (2 mentions) Facscanto 2, by BD (2 mentions) Apc cy7 clone 3g8, by BD (1 mentions) Ack lysis buffer, by Lonza (1 mentions)

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MAL I (6 citations)

2 protocols using fluorescein mal 1

1

Multiparametric flow cytometry analysis

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Blood samples were collected in tubes coated with an anticoagulant via venipuncture. PBLs were prepared by lysing erythrocytes through incubation of blood in ammonium-chloride-potassium (ACK) lysis buffer (Lonza) and stained using a premix antibody cocktail containing various human antibodies as indicated in the gating procedure, along with either Fluorescein-MAL-1 (Vector Labs) or Fluorescein-SNA (Vector Labs). The stained cells were read using a BD FACS Canto II flow cytometry system (BD Biosciences) and analyzed using FlowJo software. PBLs were gated based on their cell surface staining profiles, CD3+ (anti-human CD3 conjugated to PE-Cy7, clone SK7, BD Biosciences), CD19+ (anti-human CD19 conjugated to PE, clone HIB19, BD Biosciences), CD15+CD16+ (anti-human CD15 conjugated to APC, clone HI98, BD Biosciences; anti-human CD16 conjugated to APC-Cy7, clone 3G8, BD Biosciences), and CD14+ (anti-human CD14 conjugated to PerCP-Cy5.5, clone M5E2, BD Biosciences) for T cells, B cells, neutrophils, and monocytes/macrophages, respectively.
Nguyen T., Lee S., Yang Y.A., Ahn C., Sim J.H., Kei T.G., Barnard K.N., Yu H., Millano S.K., Chen X., Parrish C.R, & Song J. (2020). The role of 9-O-acetylated glycan receptor moieties in the typhoid toxin binding and intoxication. PLoS Pathogens, 16(2), e1008336.
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2

Quantification of Sialosides on Immune Cells

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PBLs were stained using a premix antibody cocktail containing various human and mouse antibodies (Key Resources Table), along with either Fluorescein-MAL-1 (Vector Labs) or Fluorescein-SNA (Vector Labs). The stained cells were read using a BD FACS Canto II flow cytometry system (BD Biosciences) and analyzed using FlowJo software. Murine PBLs were gated based on their cell surface staining profiles, CD3+, CD19+, CD11b+Ly6G+ (mouse) or CD15+CD16+ (human), or CD11b+CD11c+ (mouse) or CD14+ (human) and the surface expressions of α2–3 and α2–6 sialosides on T cells, B cells, neutrophils, and monocytes were quantified by analyzing their co-staining profiles with MAL-1 or SNA.
Lee S., Yang Y.A., Milano S.K., Nguyen T., Ahn C., Sim J.H., Thompson A.J., Hillpot E.C., Yoo G., Paulson J.C, & Song J. (2020). Salmonella Typhoid Toxin PltB Subunit and Its Nontyphoidal Salmonella Orthologue Confer Differential Host Adaptation and Virulence. Cell host & microbe, 27(6), 937-949.e6.
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