B6.129s4 tet1tm1 1jae j

Manufactured by Jackson ImmunoResearch

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B6.129S4-Tet1tm1.1Jae/J is a genetically modified mouse strain. It carries a targeted mutation in the Tet1 gene, which is involved in DNA methylation and gene expression regulation. This strain can be used for research purposes related to the study of Tet1 and its functions.

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B6 cg tg prrx1 cre 1cjt j, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

2 protocols using b6.129s4 tet1tm1 1jae j

Mouse Models for Epigenetic Regulation Studies

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Female C57BL/6J (JAX #000664), B6.129S4-Tet1^tm1.1Jae/J (JAX #017358, Tet1^+/−), B6.129S-Tet2^tm1.1laai/J (JAX #017573, Tet2^fl/fl), and B6.Cg-Tg (Prrx1-cre)1Cjt/J (JAX #005584, Prx1^cre) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Age-matched littermates were used in all experiments. The 8–10-week-old C57BL/6J mice were ovariectomized (OVX)^{22 (link)}, and sham-operated age-matched female mice served as controls. We purchased immunocompromised nude mice (Beige nu.nu XIDIII) from Harlan (Indianapolis, IN, USA). To generate Tet1^−/−Prx1^creTet2^fl/fl mice, we mated Tet1^+/−Prx1^creTet2^fl/+ mice with Tet1^+/−Tet2^fl/+ mice, and littermates whose genetic status was Prx1^cre were used as controls. All animal experiments were performed under institutionally approved protocols for the use of animal research (University of Pennsylvania Institutional Animal Care and Use Committee #805478).

Yang R., Yu T., Kou X., Gao X., Chen C., Liu D., Zhou Y, & Shi S. (2018). Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter. Nature Communications, 9, 2143.

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Tet1 Heterozygous Mice Behavioral Study

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Tet1+/− mice were purchased from The Jackson Laboratory (Strain Name: B6;129S4-Tet1tm1.1Jae/J, Stock Number: 017358) and were bred as heterozygotes at the University of Alabama at Birmingham. WT and KO male offspring of the heterozygote parents of age between 3–4 months were used for all the behavior and molecular experiments. For the Object Location Memory task, 10–12 week old C57BL/6 male mice from Harlan were injected rAAVs through stereotaxic surgeries. Animals were singly housed three days before the start of any behavior experiments. Animals were maintained under a 12h light/dark schedule with access to food and water ad libitum. All studies were performed in compliance with the University of Alabama Institutional Animal Care and Use Committee guidelines.

Kumar D., Aggarwal M., Kaas G.A., Lewis J., Wang J., Ross D.L., Zhong C., Kennedy A., Song H, & Sweatt J.D. (2015). Tet1 Oxidase Regulates Neuronal Gene Transcription, Active DNA Hydroxy-methylation, Object Location Memory, and Threat Recognition Memory. Neuroepigenetics, 4, 12-27.

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