Acetic acid (AA), acetonitrile (ACN), atovaquone, dimethyl sulfoxide (DMSO), formic acid (FA), methanol (MeOH), and water were purchased from Fisher Scientific (Pittsburgh, PA). Glycine, 2,2-d2-Glycine, leucine, 1-13C, 15N-leucine, 1,2-13C2-leucine, 2-13C, 15N-leucine, formaldehyde, formaldehyde-d2 solution, sodium cyanoborohydride, sodium cyanoborodeuteride, triethylammonium bicarbonate buffer (TEAB, 1.0 M), tris(2-carboxy-ethyl) phosphine hydrochloride (TCEP), RPMI-1640 medium were purchased from Sigma-Aldrich (St. Louis, MO). Maltooctaose was purchased from Toronto Research Chemicals (Toronto, Canada). PNGase F was purchased from Promega (Madison, WI). Bovine thyroglobulin (BTG) and human IgG subtypes were purchased from Thermo Fisher Scientific (Rockford, IL). ECC1 cells were obtained from ATCC (Manassas, VA). Oasis HLB 1cc cartridges were purchased from Waters Corporation (Milford, MA). Microcon-30kDa centrifugal filters (30K MWCO) were purchased from Merck Millipore Ltd. (Darmstadt, Germany). PolyGLYCOPLEX A™ beads (3 μm) were purchased from PolyLC Inc. (Columbia, MD). Fused silica capillary tubing (i.d., 75 μm, o.d., 375 μm) was purchased from Polymicro Technologies (Phoenix, AZ). All reagents were used without additional purification.
15n leucine
Manufactured by Merck Group
Sourced in United States
15N-leucine is a stable isotope-labeled amino acid. It is a form of the essential amino acid leucine in which one of the nitrogen atoms has been replaced with the heavier isotope nitrogen-15. This stable isotope can be used as a tracer in various biochemical and biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Leucine, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Acetic acid, by Thermo Fisher Scientific (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Fused silica capillary tubing, by Molex (2 mentions)
Formaldehyde, by Merck Group (2 mentions)
Sodium cyanoborohydride, by Merck Group (2 mentions)
Sodium cyanoborodeuteride, by Merck Group (1 mentions)
Microcon 30 kda centrifugal filters 30k mwco, by Merck Group (1 mentions)
Ecc1 cells, by American Type Culture Collection (1 mentions)
Oasis hlb 1 cc cartridges, by Waters Corporation (1 mentions)
Glycine, by Merck Group (1 mentions)
Pngase f, by Promega (1 mentions)
Hydroxybenzotriazole, by Merck Group (1 mentions)
Dicyclohexylcarbodiimide, by Merck Group (1 mentions)
Lys c, by Promega (1 mentions)
Water 18o, by Merck Group (1 mentions)
13c formaldehyde, by Merck Group (1 mentions)
5 protocols using 15n leucine
1
Glycopeptide Analysis by Mass Spectrometry
2
Isotopically-Labeled Amino Acid Standards
3
Synaptotagmin 1 Vesicle Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We added 2.4 mM 15N leucine (Sigma‐Aldrich) to the neuronal culture medium (0.8 mM leucine) for 1–3 days prior to antibody tagging of releasable vesicles with the 604.2 Synaptotagmin 1 antibody, or for 3 days following tagging with the 604.2 Synaptotagmin 1 antibody to assess the inactive vesicle population. The cultures were then fixed, processed into 200‐nm‐thin sections, and imaged in fluorescent microscopy, before imaging in nanoSIMS and correlating the images as described before (Saka et al, 2014b). A co‐immunostaining for Synaptophysin and PSD95 was used to identify synapses.
4
Dietary Isotope Labeling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The food was mixed with 0.5 mL of a solution of 0.18 M 15N‐leucine (Sigma‐Aldrich) and 0.3 mL of vegetable oil per gram of a commercial pellet (size 50, Ewos, for composition, see Table 1 ). The 15N‐leucine food was dried for 24 hours at 30°C.
5
Stable Isotope Tracer Analysis of Leucine Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals, namely leucine, l -[13C6, 15N]leucine, sodium 4-methyl-2-oxovalerate, sodium 2-oxoisocaproate, and citric acid, were purchased from (Sigma St. Louis, MO, USA). HPLC-grade water, acetonitrile, and ammonium acetate were purchased from Sigma. Both antibodies, rabbit anti-MCC [26 (link),27 (link)], and human anti-pyruvate dehydrogenase (PDH) [27 (link),37 (link)], were a gift from Prof. Dr. Bernd Hamprecht (IFIB, University of Tuebingen, Tübingen, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!