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Anti b220 cd45r clone ra3 6b2

Manufactured by Thermo Fisher Scientific
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Anti-B220/CD45R (clone RA3-6B2) is a monoclonal antibody that binds to the B220/CD45R antigen, which is expressed on the surface of B cells. This antibody can be used for the identification and isolation of B cells in flow cytometry and other applications.

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Lab products found in correlation

Anti cd45 clone 30 f11, by BioLegend (2 mentions) Cd16 cd32 fc block clone 93, by BioLegend (2 mentions) Anti cd3ε clone 145 2c11, by BioLegend (2 mentions) Anti ly 6g clone 1a8, by Thermo Fisher Scientific (2 mentions) Anti siglecf clone e50 2440, by BD (2 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd11c clone n418, by BioLegend (2 mentions) Anti cd11b clone m1 70, by BioLegend (2 mentions)

Close spelling variants of this product

CD45R/B220 (RA3-6B2) Anti-B220 (clone RA3-6B2; B220 (clone RA3-6B2; Anti-B220 (RA3-6B2) B220 (RA3-6B2, Clone RA3-6B2 Anti-CD45R/B220 RA3-6B2 CD45R/B220 Anti-B220

2 protocols using anti b220 cd45r clone ra3 6b2

1

Comprehensive Lung Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lung tissue was harvested as indicated and single cell suspension were prepared using a metal mesh. Absolute cell numbers were counted with a Neubauer chamber. Single-cell suspensions of the lungs respectively collected BAL fluid cells were incubated with CD16/CD32 Fc block (clone 93, BioLegend, 101310) to inhibit unspecific antibody binding. For flow cytometry, cells were stained with the following antibodies: anti-B220/CD45R (clone RA3-6B2, eBioscience, 45-0452), anti-CD3ε (clone 145-2C11, BioLegend, 100320), anti-CD11b (clone M1/70, BioLegend, 101206), anti-Ly-6G (clone 1A8, eBioscience, 17-9668), anti-CD45 (clone 30-F11, BioLegend, 103137), anti-CD11c (clone N418, Biolegend, 117333) and anti-SiglecF (clone E50-2440, BD, 562681). To exclude dead cells from the analysis, the samples were labeled with the Fixable Viability Dye eFluor 780 (eBioscience, 65-0865).
Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.
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2

Comprehensive Lung Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lung tissue was harvested as indicated and single cell suspension were prepared using a metal mesh. Absolute cell numbers were counted with a Neubauer chamber. Single-cell suspensions of the lungs respectively collected BAL fluid cells were incubated with CD16/CD32 Fc block (clone 93, BioLegend, 101310) to inhibit unspecific antibody binding. For flow cytometry, cells were stained with the following antibodies: anti-B220/CD45R (clone RA3-6B2, eBioscience, 45-0452), anti-CD3ε (clone 145-2C11, BioLegend, 100320), anti-CD11b (clone M1/70, BioLegend, 101206), anti-Ly-6G (clone 1A8, eBioscience, 17-9668), anti-CD45 (clone 30-F11, BioLegend, 103137), anti-CD11c (clone N418, Biolegend, 117333) and anti-SiglecF (clone E50-2440, BD, 562681). To exclude dead cells from the analysis, the samples were labeled with the Fixable Viability Dye eFluor 780 (eBioscience, 65-0865).
Schliehe C., Flynn E.K., Vilagos B., Richson U., Swaminanthan S., Bosnjak B., Bauer L., Kandasamy R.K., Griesshammer I.M., Kosack L., Schmitz F., Litvak V., Sissons J., Lercher A., Bhattacharya A., Khamina K., Trivett A.L., Tessarollo L., Mesteri I., Hladik A., Merkler D., Kubicek S., Knapp S., Epstein M.M., Symer D.E., Aderem A, & Bergthaler A. (2014). The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection. Nature immunology, 16(1), 67-74.
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