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Aew541

Manufactured by Selleck Chemicals
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Sourced in United States

The AEW541 is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of the AEW541 is to perform specific tasks related to the handling and processing of samples or materials in a laboratory setting. No further details are available regarding the intended use or features of this product.

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Lab products found in correlation

Lapatinib, by Selleck Chemicals (3 mentions) Erlotinib, by Selleck Chemicals (2 mentions) Trametinib, by Selleck Chemicals (2 mentions) Ldk378, by Selleck Chemicals (2 mentions) Crizotinib, by Chemietek (2 mentions) Mab391, by R&D Systems (2 mentions) Osi 906, by Selleck Chemicals (2 mentions) Sorafenib, by Selleck Chemicals (1 mentions) Temsirolimus, by Selleck Chemicals (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) U0126, by Selleck Chemicals (1 mentions) Trypsin, by Sartorius (1 mentions) Phosphatase inhibitor cocktails 2 and 3, by Merck Group (1 mentions) Mouse anti parp 1, by Cell Signaling Technology (1 mentions) Goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Rabbit anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Rabbit anti erk1 2, by Cell Signaling Technology (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Selumetinib, by Selleck Chemicals (1 mentions)

6 protocols using aew541

1

Dual PI3K/mTOR and IGF1R Inhibitor Preparation

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Dual PI3K/mTOR inhibitor BGT226 (NVP-BGT226, cat# S2749)62 (link) and small molecule IGF1R inhibitor AEW541 (NVP-AEW541, cat# S1034)63 (link) were purchased from Selleck Chemicals. Both drugs were suspended in dimethyl sulfoxide (DMSO) for in vitro applications. For in vivo use, BGT226 was suspended in N-methyl-2-pyrrolidone (NMP). Immediately before administration, the NMP/BGT226 stock solution was diluted in PEG300 (10% NMP/BGT226 plus 90% PEG300). AEW541 was suspended in L(+)-tartaric acid (25 mM) for use in mice.
May C.D., Landers S.M., Bolshakov S., Ma X., Ingram D.R., Kivlin C.M., Watson K.L., Sannaa G.A., Bhalla A.D., Wang W.L., Lazar A.J, & Torres K.E. (2017). Co-targeting PI3K, mTOR, and IGF1R with small molecule inhibitors for treating undifferentiated pleomorphic sarcoma. Cancer Biology & Therapy, 18(10), 816-826.
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2

Small Molecule Compound Preparation

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Gefitinib, AEW541,erlotinib, trametinib, U0126, sorafenib, and temsirolimus were purchased from Selleckchem and dissolved in 100% DMSO to generate 100mM stock solutions of each, stored at −80′C. For erlotinib, the 100mM stock solution was further diluted to 30mM in 100% DMSO for complete solubility. Novel compounds were provided by Dr. Chris Ireland and Dr. Sunil Sharma at the University of Utah.
El-Chaar N.N., Piccolo S.R., Boucher K.M., Cohen A.L., Chang J.T., Moos P.J, & Bild A.H. (2014). Genomic classification of the RAS network identifies a personalized treatment strategy for lung cancer. Molecular Oncology, 8(7), 1339-1354.
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3

Preparation and Dissolution of Kinase Inhibitors

Cited 1 time
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X-376 was prepared as described previously14 (link). Crizotinib (ChemieTek, Indianapolis, IN, USA), OSI-906 (Selleck Chemicals, Houston, TX, USA), AEW-541 (Selleck Chemicals, Houston, TX, USA), LDK-378 (Selleck Chemicals, Houston, TX, USA) and Lapatinib (Selleck Chemicals, Houston, TX, USA) were dissolved in DMSO. Erlotinib was synthesized by the Memorial Sloan-Kettering Cancer Center (MSKCC) Organic Synthesis Core. MAb391 (R&D systems, Minneapolis, MN, USA) was dissolved in PBS.
Lovly C.M., McDonald N.T., Chen H., Ortiz-Cuaran S., Heukamp L.C., Yan Y., Florin A., Ozretić L., Lim D., Wang L., Chen Z., Chen X., Lu P., Paik P.K., Shen R., Jin H., Buettner R., Ansén S., Perner S., Brockmann M., Bos M., Wolf J., Gardizi M., Wright G.M., Solomon B., Russell P.A., Rogers T.M., Suehara Y., Red-Brewer M., Tieu R., de Stanchina E., Wang Q., Zhao Z., Johnson D.H., Horn L., Wong K.K., Thomas R.K., Ladanyi M, & Pao W. (2014). Rationale for co-targeting IGF-1R and ALK in ALK fusion positive lung cancer. Nature medicine, 20(9), 1027-1034.
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4

Preparation and Dissolution of Kinase Inhibitors

Cited 1 time
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X-376 was prepared as described previously14 (link). Crizotinib (ChemieTek, Indianapolis, IN, USA), OSI-906 (Selleck Chemicals, Houston, TX, USA), AEW-541 (Selleck Chemicals, Houston, TX, USA), LDK-378 (Selleck Chemicals, Houston, TX, USA) and Lapatinib (Selleck Chemicals, Houston, TX, USA) were dissolved in DMSO. Erlotinib was synthesized by the Memorial Sloan-Kettering Cancer Center (MSKCC) Organic Synthesis Core. MAb391 (R&D systems, Minneapolis, MN, USA) was dissolved in PBS.
Lovly C.M., McDonald N.T., Chen H., Ortiz-Cuaran S., Heukamp L.C., Yan Y., Florin A., Ozretić L., Lim D., Wang L., Chen Z., Chen X., Lu P., Paik P.K., Shen R., Jin H., Buettner R., Ansén S., Perner S., Brockmann M., Bos M., Wolf J., Gardizi M., Wright G.M., Solomon B., Russell P.A., Rogers T.M., Suehara Y., Red-Brewer M., Tieu R., de Stanchina E., Wang Q., Zhao Z., Johnson D.H., Horn L., Wong K.K., Thomas R.K., Ladanyi M, & Pao W. (2014). Rationale for co-targeting IGF-1R and ALK in ALK fusion positive lung cancer. Nature medicine, 20(9), 1027-1034.
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5

Western Blot Analysis of Signaling Pathways

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Primary antibodies: mouse anti-PARP-1, rabbit anti-phospho-Ser473 Akt, rabbit anti-phospho-Thr308 Akt, rabbit anti-Akt, rabbit anti-phospho-ERK1/2, rabbit anti-ERK1/2, rabbit anti-phospho-MEK1/2, mouse anti-MEK1/2, mouse anti-p21waf1, rabbit anti-p27, were from Cell Signaling Technologies (Boston, MA, USA). Mouse anti-human GAPDH and rabbit anti-cyclin A were from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies: horseradish peroxidase (HRP)—conjugated goat anti-rabbit and goat anti-mouse IgG (H + L) antibodies were from Jackson (Baltimore Pike West Grove, PA, USA). EZ-ECL enhanced chemiluminescence detection kit, RPMI1640 medium, L-glutamine, fetal bovine serum, trypsin, antibiotics and phosphate buffered saline (PBS) were from Biological Industries (Beit-Ha-Emek, Israel). Propidium iodide, phosphatase inhibitor cocktails 2 and 3, sulphorodamine B (SRB), trichloroacetic acid and acetic acid were from Sigma Aldrich (St. Louis, MO, USA). ZSTK474, Selumetinib and AEW-541 were from Selleckchem (Houston, TX, USA). Complete mini protease inhibitor cocktail, RNAse A and Triton X-100 were from Roche Diagnostics Gmbl (Mannheim, Germany).
Cohen Z., Maimon Y., Samuels N., Brand H., Sulkes A., Brenner B, & Berger R. (2022). Strong Synergic Growth Inhibition and Death Induction of Cancer Cells by Astragalus membranaceus and Vaccaria hispanica Extract. Cancers, 14(23), 5833.
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6

Comprehensive cancer drug evaluation

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Tumor cell lines were obtained from ATCC and were cultured in RPMI1640 (Invitrogen) supplemented with 10% fetal bovine serum (Millipore). Erlotinib, afatinib, neratinib, GDC-0941, trametinib, lapatinib, insitinib, AEW541, imatinib, BGJ398, crizotinib and foretinib were purchased from Selleck Chemicals. All inhibitors were reconstituted in DMSO (Sigma-Aldrich) at a stock concentration of 10 mM.
Ma P., Fu Y., Chen M., Jing Y., Wu J., Li K., Shen Y., Gao J.X., Wang M., Zhao X, & Zhuang G. (2016). Adaptive and Acquired Resistance to EGFR Inhibitors Converge on the MAPK Pathway. Theranostics, 6(8), 1232-1243.
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