CHA nanorods were examined by transmission electron microscope (TEM, Tecnai 12, Philips, Amsterdam, Holland) and selected electron diffraction (SEAD, Tecnai 12, Philips, Amsterdam, Holland) for structural characterization. The size distribution of nanoparticles from TEM micrographs was measured by Nano measurer software (1.2). In total, 88 points were selected to analyze the size distribution of HA nanorods. The molecular structure of CHA was analyzed by Raman spectroscopy (DXR, GX-PT-2412, Thermo, Waltham, MA, USA) with 532 nm laser as excitation wavelength. XRD spectrum was recorded on an X-ray powder diffractometer using CuKα radiation operating at 40 kV and 30 mA (XRD, D8 ADVANCE, Bruker-AXS, Bremen, Germany). The elements composition of the sample was analyzed by X-ray photo-electronic spectroscopy (XPS, ESCALAB250Xi, Thermo Fisher Scientific, Waltham, MA, USA), using a monochromated Al Kα X-ray source. The samples were scanned at a reflection angle (2θ) using a step rate of 1.0 deg/min. Fourier transform infrared spectrometry (FTIR, ALPHA, Bruker, Blaireka, MA, USA) was used to identify the molecular structure of CHA nanorods. FTIR spectrum of CHA nanorods was recorded from 500 to 3600 cm−1. The surface and porosity of as-prepared CHA nanorods were characterized by Brunauer-Emmett-Teller (BET) analyzer (ASAP 2020 HD88, Micromeritics, Norcross, GA, USA).
Tecnai 12
Manufactured by Philips
Sourced in Netherlands, United States
The Tecnai 12 is a transmission electron microscope (TEM) manufactured by Philips. It is designed to provide high-resolution imaging and analysis of a wide range of samples. The Tecnai 12 is equipped with advanced optics and a high-performance electron gun to deliver clear and detailed images of specimens at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (8 mentions)
D8 advance, by Bruker (7 mentions)
S 4800ii, by Hitachi (7 mentions)
S 4800, by Hitachi (5 mentions)
Tecnai g2 f30 s twin, by Thermo Fisher Scientific (4 mentions)
Geminisem 300, by Zeiss (3 mentions)
Raman spectrometer, by Renishaw (3 mentions)
Bca protein assay kit, by CWBIO (3 mentions)
Cary 5000, by Agilent Technologies (3 mentions)
Nano z, by Malvern Panalytical (3 mentions)
Av 500, by Bruker (2 mentions)
Zeta plus, by Malvern Panalytical (2 mentions)
Em uc7, by Leica camera (2 mentions)
Ultracut uct, by Leica camera (2 mentions)
Zetasizer nano z, by Malvern Panalytical (2 mentions)
Xsam 800 spectrometers, by Shimadzu (2 mentions)
Optima l 100 xp ultracentrifuge, by Beckman Coulter (2 mentions)
Attune nxt, by Thermo Fisher Scientific (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
152 protocols using tecnai 12
1
Comprehensive Structural Analysis of CHA Nanorods
2
Characterization and Evaluation of Liposome Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The
dimensions, Đ and surface charge (ξ-potential), of the
liposome formulation at a scattering angle of 90° were measured
by Master size 2000 DLS. Each sample was repeatedly measured three
times at room temperature.
The liposome morphology was analyzed
by TEM (Jem-2100F, JEOL, Japan) operated at 200 kV, and BIOTEM (Tecnai
12, Philips, Holland) operated at 80 kV, respectively.
EE and
DL are essential indicators for assessing the preparation
technology and quality of liposomes. To determine these indicators,
Sephadex G-50 gel column was used with a volume of 20 cm–3 and a diameter of Sephadex G-50 gel column. The prepared liposomes
were added to the column and eluted with PBS eluent at a flow rate.
The collected eluent was desulfonated with methanol, and the drug
content was determined by HPLC. Then the EE and DL were calculated
by the following formula.
The liposomes were stored at 4 °C
in the dark for one month.
Subsequently, the stability of liposomes during storage was assessed
by measuring the particle size, Đ, and zeta potential of liposomes
before and after storage.
dimensions, Đ and surface charge (ξ-potential), of the
liposome formulation at a scattering angle of 90° were measured
by Master size 2000 DLS. Each sample was repeatedly measured three
times at room temperature.
The liposome morphology was analyzed
by TEM (Jem-2100F, JEOL, Japan) operated at 200 kV, and BIOTEM (Tecnai
12, Philips, Holland) operated at 80 kV, respectively.
EE and
DL are essential indicators for assessing the preparation
technology and quality of liposomes. To determine these indicators,
Sephadex G-50 gel column was used with a volume of 20 cm–3 and a diameter of Sephadex G-50 gel column. The prepared liposomes
were added to the column and eluted with PBS eluent at a flow rate.
The collected eluent was desulfonated with methanol, and the drug
content was determined by HPLC. Then the EE and DL were calculated
by the following formula.
The liposomes were stored at 4 °C
in the dark for one month.
Subsequently, the stability of liposomes during storage was assessed
by measuring the particle size, Đ, and zeta potential of liposomes
before and after storage.
3
Transmission Electron Microscopy of HMNs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The morphology of the HMNs was observed using transmission electron microscopy (TEM; Philips Tecnai 12) operated at 120 kV. According to the process described by Forte and Nordhausen45 (link) with some modifications, samples were diluted with ammonium acetate buffer (0.125 M ammonium acetate, 2.6 mM ammonium carbonate, 0.26 mM tetrasodium EDTA, pH 7.4). Then, a drop of the diluted samples at a final protein concentration of 0.5 mg/mL was applied to carbon-coated 100-mesh copper grids and adsorbed for approximately 5 minutes, followed by negative staining with a drop of phosphotungstic acid (2% v/v). Excess solutions were blotted by filter papers, and the grids were air-dried at room temperature before observation under TEM.
4
Characterization of DA-Nanoparticle Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The prepared optimized DA-nanoparticles formulations were characterized for particle size, Polydispersity Index (PDI) and Zeta potential using Zetasizer. For assessing the relevant parameters of the formulations, the average particle count rate was kept between 30 and 80 kcps. Assessment of the shape and surface morphology were conducted using the Transmission Electron Microscopy (TEM). One drop of the nano-emulsion was placed on copper grids and stained with 2% (w/v) phosphotungstic acid for 5 min at room temperature. Finally, images were produced by employing a TEM instrument (Tecnai-12, Philips) to observe the grids bearing nanoemulsion. Mean diameter and size distribution of nanoparticles were determined using a Zetatrac Ultra at 25 °C. Volume-distribution curves were used to represent the size distribution.
5
Comprehensive Materials Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All samples were analyzed using a scanning electron microscope (SEM, Hitachi, S-4800 II, Tokyo, Japan), transmission electron microscope (TEM), high-resolution transmission electron microscope (HRTEM, Philips, TECNAI 12, Amsterdam, The Netherlands), and the related element mapping analysis (200 kV Philips TECNAI G2 electron microscope). The KEVEX X-ray energy detector was used for energy color scattered X-ray (EDS) analysis. Powder X-ray diffraction (XRD) patterns were recorded on a Bruker D8 Advance powder using a Cu Kα (λ = 1.5405 Å) radiation source, operating at 40 kV and 40 mA at a scanning rate of 5° min−1. Surface analysis of the sample was studied by X-ray photoelectron spectroscopy (XPS, Thermo Science, ESCALAB 250Xi, Waltham, MA, USA). Thermogravimetric analysis (TGA) was carried out on a NETZSCH TG 209 F3 with a heating rate of 10 °C min−1 from room temperature to 600 °C under a N2 atmosphere.
6
Photoswitchable HA-AZO Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HA-AZO was characterized by 1H nuclear magnetic resonance (1H NMR, Bruker, AV500) using D2O as solvent. HA-AZO water solution, AZO water solution, and AZO DMSO solution was characterized by UV spectroscopy (Cary 50). UV lamp (10 W) was used as photosource to induce tran-to-cis transition of AZO domain. In order to track the structural change of molecules, real-time UV spectra as a function of irradiation time and recovery time was recorded. Repeated irradiation and recovery method were applied to demonstrate the fatigue resistance of molecules. Macromolecule status in water solution was characterized by dynamic light scattering (DLS, nano ZS) and transmission electron microscope (TEM, Philips, Tecnai 12).
7
Extracellular Vesicles of Drug-Resistant Staphylococcus aureus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Drug-resistant S. aureus BW15 and BWMR26 28 (link) and drug-sensitive S. aureus S29213 were cultured on Luria broth (LB) agar overnight at 37 °C and then a single colony was inoculated into LB medium on a rotary shaker. Then, a 1:100 dilution of the bacteria were cultured at 37 °C in LB medium until they reached late-logarithmic-phase. The bacterial culture was centrifuged at 6000 × g for 20 min to remove the bacteria, followed by filtering the medium through a 0.45 μm vacuum filter. The medium was then centrifuged at 150,000 × g for 2 h at 4 °C (Beckman Coulter, California, USA). The precipitates were considered the EV pellets 24 (link). EV13, EV15, and EV26 were from S. aureus S29213, S. aureus BW15 and S. aureus BWMR26, respectively.
The EV particle morphology was monitored by transmission electron microscopy (TEM; Tecnai 12, Philips, Holland). The hydrodynamic size and potential were measured by Zeta Plus (Malvern Instruments, Worcestershire, UK). The production of EVs from each S. aureus strain was determined using a BCA protein assay and was shown as the total protein (mg) in EVs derived from 1 L of late-logarithmic-phase cultures. The total proteins in the EVs derived from different S. aureus strains were analyzed by SDS-PAGE and the protein abundance of the EV was analyzed by ImageJ 29 (link).
The EV particle morphology was monitored by transmission electron microscopy (TEM; Tecnai 12, Philips, Holland). The hydrodynamic size and potential were measured by Zeta Plus (Malvern Instruments, Worcestershire, UK). The production of EVs from each S. aureus strain was determined using a BCA protein assay and was shown as the total protein (mg) in EVs derived from 1 L of late-logarithmic-phase cultures. The total proteins in the EVs derived from different S. aureus strains were analyzed by SDS-PAGE and the protein abundance of the EV was analyzed by ImageJ 29 (link).
8
Transmission Electron Microscopy of Cerebral Cortex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the transmission electron microscopy observations, small 1 mm3 blocks containing cerebral cortex samples from each group were immediately fixed in ice-cold 2.5% glutaraldehyde overnight at 4 °C. The pieces were rinsed three times in phosphate-buffered saline (PBS) and then post-fixed in 1% osmium tetroxide. Thereafter, the samples were washed three times with PBS, dehydrated in graded solutions of ethanol, and embedded in an epoxy resin. An ultramicrotome (EM UC7, Leica, Germany) was used to obtain ultrathin sections, which were then stained with uranyl acetate and lead citrate. The sections were subsequently examined using a transmission electron microscope (Tecnai 12, Philips, Holland).
9
Histological and Ultrastructural Analysis of Colonic Mucosa
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Distal segments of the colon were cut into 1 cm portions and fixed, and 5 µm-thick histological sections were mounted on gelatinized glass slides, dewaxed in xylene, dehydrated using graded concentrations of alcohol, and finally stained with hematoxylin and eosin (H&E).
Samples were immediately fixed with 2.5% glutaraldehyde in 0.1 mol/L sodium cacodylate buffer (pH 7.4) for 2 hours at 22 ℃, then rinsed for 18 hours at 4 ℃ with 0.05 M Tris buffer (pH 7.6) and washed 3 times for 5 minutes each time. The ultrastructure of the colonic mucosa was observed by H-6600 transmission electron microscopy (Philips Tecnai-12, Amsterdam, Netherlands).
Samples were immediately fixed with 2.5% glutaraldehyde in 0.1 mol/L sodium cacodylate buffer (pH 7.4) for 2 hours at 22 ℃, then rinsed for 18 hours at 4 ℃ with 0.05 M Tris buffer (pH 7.6) and washed 3 times for 5 minutes each time. The ultrastructure of the colonic mucosa was observed by H-6600 transmission electron microscopy (Philips Tecnai-12, Amsterdam, Netherlands).
10
Characterization of Silica Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The powder of SiNPs was dispersed in sterilized water (final concentration: 4 mg/mL) and stored at 4 °C until use. The morphology and average size of SiNPs were determined by a transmission electron microscope (TEM; Tecnai 12; Royal Philips, Amsterdam, Holland) using dropping aliquots of SiNP solutions on 400-mesh carbon-coated copper grids (SPI Supplies, West Chester, PA, USA). The zeta potential of SiNPs were examined by a Zetasizer 3000HS (Malvern Instruments Ltd., Malvern, UK). Before experiment use, SiNPs in the stock suspension were dispersed using a sonicator (160 W, 20 kHz, 30 min; Bioruptor UCD-200, Belgium).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!