Mda mb 468

Human breast cancer cell lines of MCF-7, T-47D, MDA-MB-231 and MDA-MB-468 were obtained from Shanghai Cell Bank (Chinese Academy of Science, Shanghai, China). All cell culture reagents were purchased from GIBCO (Invitrogen, Grand Island, NY, USA). MCF-7 and MDA-MB-231 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. T-47D and MDA-MB-468 cells were cultured in RPMI-1640 medium containing 10% FBS and 1% penicillin/streptomycin. All cell lines were maintained in a humidified atmosphere containing 5% CO₂ at 37 °C. Cells in the logarithmic phase were irradiated with 4 Gy X-rays by using the X-ray Irradiator (X-RAD 320, PXI, Madison, CT, USA) at a dose rate of 1 Gy/min. After 12 h of irradiation, cells were collected for further assays.

The breast cancer cell lines, MDA-MB-231, MCF-7, MDA-MB-468, SKBR3 and BT549 were purchased from Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China). MDA-MB-231, BT549 and SKBR3 cell lines were grown in RPMI1640 medium (Hyclone, Massachusetts, USA) supplemented with 10% FBS (Gibco-BRL) at 37°C in a 5% CO₂ atmosphere. MDA-MB-468 and MCF-7 were grown in DMEM medium (Gibco) supplemented with 10% FBS (Gibco) at 37°C in a 5% CO₂ atmosphere.

Normal human mammary epithelial cells (MCF10A) and the accepted BC cell lines (MDA-MB-468, SKBR3, MCF7 and MDA-MB-231) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cells were allowed to grow in Dulbecco modified Eagle medium supplemented with 10% (v/v) fetal bovine serum (PAN-Biotech, Aidenbach, Germany), 1% penicillin–streptomycin antibiotics (Beyotime, Shanghai, China) in a humidified atmosphere containing 5% CO₂ at 37°C. DMEM medium was changed every third day.

Human K562 myeloid lymphoblastoma cell line, human triple-negative breast cancer cell lines MDA-MB-231 and MDA-MB-468 were received from the Cell Bank of Shanghai (Shanghai in China) and maintained in (RPMI)-1640 supplemented with 10% fetal calf serum (FCS). The cells were incubated at 37°C in a humidified atmosphere containing 5% CO₂. Ubenimex (Cat. B8385) and 2′, 7′-Dichlorofluorescin diaceta (DCFH-DA) were purchased from Sigma. Paclitaxel (PTX, Cat. SP8020) and doxorubicin (Epirubicin Hydrochloride, Cat. IE 0640) were purchased from Solarbio. Carboplatin (CBP, Cat.C-0219027) and Cyclophosphamide (CTX, Cat.C-0232450) were purchased from HEOWNS. All the compounds were dissolved in sterile dimethyl sulfoxide (DMSO) to constitute 20 mM stock agents and the aliquots were stored at -20°C.

A normal human breast epithelial cell line Hs 578Bst and four breast cancer cell lines (MDA-MB-415, MDA-MB-231, MDA-MB-468 and MCF7) were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). All the cells were cultured in DMEM (Gibco, Grand Island, New York, U.S.A.) supplemented with 10% fetal bovine serum (FBS, Sigma–Aldrich, St. Louis, MO, U.S.A.). Cells were maintained at 37°C in a humidified atmosphere containing 5% CO₂.