Miconazole nitrate USP (≥99%) was sourced from Gufic Biosciences Limited (Mumbai, India). Oleic acid USP XVI was acquired from Union Chemical 1986 Co., Ltd. (Bangkok, Thailand). Caprylic/capric triglyceride was obtained from Sasol Olefins & Surfactants GmbH (Hamburg, Germany). PSM and sorbitan monooleate (SM) were purchased from S. Tong Chemicals Co., Ltd. (Bangkok, Thailand). DEGEE was sourced from Sigma Chemical Company (St. Louis, MO, USA). Additionally, 2-propanol and ethanol (95% v/v) were purchased from RCI Labscan Limited (Samutsakorn, Thailand). Coomassie brilliant blue R-250 was procured from Bio-Rad Laboratories (Hercules, CA, USA), while phosphate buffered saline (PBS) tablets utilized for the preparation of pH 7.4 buffer solution were acquired from Sigma Chemical Company (St. Louis, MO, USA). Sabouraud dextrose agar (SDA) was obtained from Scharlab, S.L. (Barcelona, Spain). Methanol (HPLC grade) was sourced from Honeywell International Inc. (Seoul, Republic of Korea). During the course of the experiments, DI water was utilized. Additionally, other chemicals and reagents of analytical grade or higher were procured from regional suppliers and utilized without the need for additional purification.
2 propanol
Manufactured by RCI Labscan
Sourced in Thailand
2-propanol is a colorless, flammable liquid chemical compound. It is commonly used as a solvent, cleaning agent, and disinfectant in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline tablet, by Merck Group (1 mentions)
Sabouraud dextrose agar, by Scharlab (1 mentions)
Coomassie brilliant blue r 250, by Bio-Rad (1 mentions)
Hplc grade methanol, by Honeywell (1 mentions)
Nitric acid, by Thermo Fisher Scientific (1 mentions)
Squalene, by Tokyo Chemical Industry (1 mentions)
Sesamol, by Merck Group (1 mentions)
Acetonitrile, by RCI Labscan (1 mentions)
N hexane, by RCI Labscan (1 mentions)
Tetrahydrofuran, by RCI Labscan (1 mentions)
Quercetin hydrate, by Merck Group (1 mentions)
Hplc grade solvents, by RCI Labscan (1 mentions)
4 protocols using 2 propanol
1
Miconazole Nitrate Topical Formulation
2
Synthesis of Mn-doped TiO2 Nanoparticles
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Undoped TiO2 and
Mn-doped TiO2 nanoparticles were prepared
by the sol–gel method. 50 mL of titanium(IV)isopropoxide (TTIP:
97% reagent grade, Sigma-Aldrich) was added to 50 mL of 2-propanol
(99.8%, RCI Labscan), and the solution was continuously stirred. Various
amount of manganese chloride (MnCl2·4H2O; 98.0%, Ajax Finechem Australia), including 0, 0.029, 0.058, and
0.087 grams, were dissolved in 10 mL of deionized water and added
to the TTIP solution. The pH of the mixture was adjusted to 2 by nitric
acid (70%, Ajax Finechem Australia). After 4 h of stirring, the mixture
was kept at 55 °C for 24 h and calcined at 400 °C for 2
h. Finally, undoped TiO2, 0.1, 0.2, and 0.3% Mn-doped TiO2 were achieved.
Mn-doped TiO2 nanoparticles were prepared
by the sol–gel method. 50 mL of titanium(IV)isopropoxide (TTIP:
97% reagent grade, Sigma-Aldrich) was added to 50 mL of 2-propanol
(99.8%, RCI Labscan), and the solution was continuously stirred. Various
amount of manganese chloride (MnCl2·4H2O; 98.0%, Ajax Finechem Australia), including 0, 0.029, 0.058, and
0.087 grams, were dissolved in 10 mL of deionized water and added
to the TTIP solution. The pH of the mixture was adjusted to 2 by nitric
acid (70%, Ajax Finechem Australia). After 4 h of stirring, the mixture
was kept at 55 °C for 24 h and calcined at 400 °C for 2
h. Finally, undoped TiO2, 0.1, 0.2, and 0.3% Mn-doped TiO2 were achieved.
3
Quantification of Bioactive Compounds
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Standard sesamin (PubChem CID: 72307, purity ≥ 98%) and Sesamolin (PubChem CID: 101746, purity ≥ 98%) were purchased from Biopurity Phytochemicals Ltd. (Chengdu, China). Sesamol (PubChem CID: 68289, purity ≥ 97%) was purchased from Sigma Aldrich Corp., Ltd. (St. Louis, MO, USA). L-Asarinin (PubChem CID: 1869417, purity ≥ 98%) was purchased from Toronto Research Chemicals (TRC, North York, ON, Canada). Standard α-, β-, γ-, and δ-tocopherols (PubChem CID: 14985, 6857447, 92729, and 92094, respectively, purity ≥ 95%) were purchased from Eisai Food & Chemical Co., Ltd. (Tokyo, Japan). Standard phytosterols (PubChem CID: 87575667, purity ≥ 95%) and squalene (PubChem CID: 637072, purity ≥ 98%) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). HPLC-grade solvents including n-hexane (PubChem CID: 8058), tetrahydrofuran (PubChem CID: 8028), acetonitrile (PubChem CID: 6342), and 2-propanol (PubChem CID: 3776) were purchased from RCI Labscan Co., Ltd. (Bangkok, Thailand).
4
HPLC Analysis of Polyphenol Compounds
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PAH16 standards as listed in Table 1 were purchased from Supelco (Bellefonte, PA, USA). HPLC-grade solvents (acetonitrile and dichloromethane) and analytical-grade solvents/chemical (methanol, 2-propanol, 1-buthanol, n-hexane and potassium hydroxide) were purchased from RCI Labscan (Bangkok, Thailand) and Ajax Finechem (Silverwater, NSW, Australia). The DADS and quercetin hydrate were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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