HepG2 and Huh7 cell lines were obtained from our laboratory for long-term storage. The cells were cultured in DMEM (high glucose) containing 10% fetal bovine serum and 1% penicillin/streptomycin. GSK461364 (S2193), volasertib (S2235), and YM155 (S1130) were purchased from Selleck. A CellTiter-Glo 2.0 cell viability assay kit (G9241) was purchased from Promega. Annexin V-FITC (Cat#: 1001) and propidium iodide (Cat#: 1056) were purchased from BioVision.
Volasertib s2235
Manufactured by Selleck Chemicals
Volasertib (S2235) is a selective inhibitor of the serine/threonine protein kinase Polo-like kinase (PLK). It is a small molecule compound used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Abcam (1 mentions)
Ym155, by Selleck Chemicals (1 mentions)
Celltiter glo 2.0 cell viability assay kit, by Promega (1 mentions)
Annexin 5 fitc, by Abcam (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Gsk461364, by Selleck Chemicals (1 mentions)
Penicillin streptomycin, by BasalMedia (1 mentions)
2 protocols using volasertib s2235
1
Cytotoxicity Evaluation of Anti-Cancer Compounds
2
Human Hepatocellular Carcinoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HCC cell lines, HEP3B217, HUH7, HEPG2, SNU398, SNU878, HUH6, SKHEP1 and PLCPRF5, were provided by Erasmus University (Rotterdam, Netherlands). MHCC97H were provided by the Liver Cancer Institute of Zhongshan Hospital (Shanghai, China). These cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (BasalMedia), incubated at 37 °C in humidified atmosphere with 5% CO2. Mycoplasma contamination was excluded via a PCR-based method. The information of TP53 mutation status of cell lines used in this study was achieved from our previous publication 54 (link). The identities of all the cell lines were confirmed by short tandem repeat (STR) profiling. Volasertib (S2235) and GSK461364 (S2193) were purchased from Selleck Chemicals and dissolved in dimethyl sulfoxide (DMSO) using a storage concentration of 10 mM.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!