A1 plus

Manufactured by Nikon

Sourced in Japan

The A1 PLUS is a high-performance microscope imaging system designed for advanced research applications. It features a modular and configurable design, allowing for customization to meet the specific needs of various research fields. The A1 PLUS provides researchers with the tools to capture, analyze, and process high-quality images and data.

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Lab products found in correlation

Rhodamine conjugated phalloidin, by Thermo Fisher Scientific (4 mentions) Imagej, by Media Cybernetics (3 mentions) Orb 4h8, by Developmental Studies Hybridoma Bank (2 mentions) Fitc conjugated or tritc conjugated anti mouse secondary antibody, by Jackson ImmunoResearch (2 mentions) Ab150073, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (2 mentions) Eclipse u2000, by Yokogawa (2 mentions) Csu10, by Yokogawa (2 mentions) In situ cell death detection kit, by Roche (2 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (2 mentions) Goat serum, by Merck Group (2 mentions) Triton x 100, by Merck Group (2 mentions) Fluoromount g, by Thermo Fisher Scientific (1 mentions) Poly l lysine hydrobromide, by Merck Group (1 mentions) Rabbit anti lc3b 2775s, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole dapi staining solution, by Beyotime (1 mentions) Antifade mounting medium, by Beyotime (1 mentions) Cy3 conjugated anti rat igg, by Merck Group (1 mentions) Fitc dextran, by Thermo Fisher Scientific (1 mentions) Jsm 7900f, by JEOL (1 mentions)

Close spelling variants of this product

Eclipse A1 Plus (10 citations) Ti-E&A1 plus (6 citations) Nikon A1 plus camera (6 citations) A1 Plus-RSi laser scanning confocal microscope (5 citations) Nikon A1 plus Ti confocal microscope (3 citations) Digital Eclipse A1 Plus microscope (3 citations) NiE-A1 plus (2 citations) Nikon A1 Plus fluorescence microscope (2 citations) A1 Resonance Plus inverted microscope (2 citations) Nikon A1 plus Ti Microscope (2 citations)

93 protocols using a1 plus

Immunofluorescence Staining of NINJ1 in Pancreatic Tissue

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Pancreatic paraffin sections (3 μm) were prepared and blocked with blank goat serum in PBST to 5%, followed by overnight incubation at 4 °C with anti-NINJ1 antibodies (1:100 dilution). The slices were then incubated with secondary antibodies (1 h, 37 °C). After cleaning with PBS, DAPI was used for nuclear staining (10 min, 37 °C) at a dilution of 1:2000. The stained sample was observed with a confocal microscope (Nikon A1plus, Tokyo, Japan) and measured at excitation and emission wavelengths of 640 nm and 700 nm. Finally, the fluorescence area was quantified using ImageJ (version 1.51k).
The isolated primary acinar cells were first exposed to STC for 50 min. They were soaked with 4% paraformaldehyde for 1 h, followed by incubation with anti-NINJ1 antibodies (dilution of 1:100) overnight at 4 °C. Subsequently, the cells were incubated with the corresponding secondary antibodies in the dark for 1 h at 37 °C. Finally, DAPI was used to counterstain the cells for 10 min. The stained specimens were visualized with a confocal microscope (Nikon A1plus, Tokyo, Japan) and measured at excitation and emission wavelengths of 640 nm and 700 nm. Finally, the fluorescence area was quantified using ImageJ (version 1.51k).

Lee C., Xin G., Li F., Wan C., Yu X., Feng L., Wen A., Cao Y, & Huang W. (2023). Calcium/P53/Ninjurin 1 Signaling Mediates Plasma Membrane Rupture of Acinar Cells in Severe Acute Pancreatitis. International Journal of Molecular Sciences, 24(14), 11554.

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Ovary Immunostaining Protocol for Drosophila

Cited 3 times

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A standard fixation and immunostaining protocol was described previously (27 , 50 (link), 59 , 68 (link), 69 (link), 79 (link)). Young female adults were mated with several male flies and fed with active dry yeast for 16~18 hours before dissection. Ovaries were dissected in 1X PBS and fixed with 4% EM-grade formaldehyde (Electron Microscopy Sciences 16% Paraformaldehyde Aqueous Solution, Fisher Scientific, Cat# 50-980-487) in 1X PBS +0.1% Triton X-100 for 20 min on a rotator; washed with 1X PBTB (1XPBS +0.1% Triton X-100+0.2% BSA) five times for 10 min each time, and blocked in 5% (vol/vol) normal goat serum-containing 1X PBTB for 1h at RT; stained with the primary mouse monoclonal anti-Orb antibody (Orb 4H8, Developmental Studies Hybridoma Bank, supernatant, 1:5) at 4 °C overnight and with the secondary FITC-conjugated or TRITC-conjugated anti-mouse secondary antibody (Jackson ImmunoResearch Laboratories, Inc; Cat# 115-095-062 and Cat# 115-025-003) at 10 µg/ml at room temperature (24~25 °C) for 4 hours; stained with rhodamine-conjugated or Alexa Fluor633-conjugated phalloidin (0.2 µg/ml), and DAPI (1 µg/mL) for >1 hour at room temperature before mounting, and imaged on a Nikon A1plus scanning confocal microscope with a GaAsP detector and a 20X 0.75 N.A. lens using Galvano scanning, controlled by Nikon Elements software. Z-stack images were acquired every 1 µm/step.

Lu W., Lakonishok M, & Gelfand V.I. (2023). Drosophila oocyte specification is maintained by the dynamic duo of microtubule polymerase Mini spindles/XMAP215 and dynein. bioRxiv.

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Immunofluorescence Analysis of E-cadherin

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For IF analysis, AGS and BGC823 cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and were then washed twice with PBS. Subsequently, cells were incubated with rabbit anti-E-cadherin (cat. no. 3195S; Cell Signaling Technology, Inc.) primary antibody at 4°C overnight. The following day, cells were washed thrice with sterile PBS and were then incubated with a conjugated anti-mouse IgG H&L (Alexa Fluor 488; cat. no. ab150113; Abcam) secondary antibody at room temperature for 1 hour. IF images were captured under a confocal microscope (Nikon A1 Plus; Nikon Corporation).

Sun Y., Du R., Shang Y., Liu C., Zheng L., Sun R., Wang Y, & Lu G. (2022). Rho GTPase-activating protein 35 suppresses gastric cancer metastasis by regulating cytoskeleton reorganization and epithelial-to-mesenchymal transition. Bioengineered, 13(6), 14605-14615.

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Immunoflourescence Imaging of Tau K18 Fibrils

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For immunofluorescence experiments, 30.000 ScN2a RML cells were plated in 12-wells plate with a 12 mm coverslip coated for 1 h with Poly-L-lysine hydrobromide (Sigma-Aldrich) 100 μg/ml. The day after, cells were treated with 2 μM Alexa-488 tau K18 fibrils and incubated for 24 h. Cells were then rinsed in PBS 1X twice and fixed with 4% paraformaldehyde in PBS for 30 min. After permeabilization with 0.1% Triton X-100, coverslips were incubated for 1 h with blocking buffer (7% normal goat serum, 1% bovine serum albumin, 0.1% Triton X-100 in PBS) and then for 1 h and 30 min with the following primary antibodies in blocking buffer (rabbit anti-calnexin ab10286 1:500 Abcam, mouse anti-M6PR ab2733 1:500 Abcam, rabbit anti-EEA1 ab2900 1:500 Abcam, rat anti-LAMP2 ab25339 1:100 Abcam, and rabbit anti-LC3B 2775s 1:200 Cell Signaling). After PBS 1X washes, coverslips were incubated with the appropriate secondary Alexa594-conjugated antibody (Invitrogen) for 1 h and nuclei counter-stained with DAPI before mounting with Fluoromount-G (Thermo Fisher Scientific). Images were acquired with Nikon confocal (Nikon A1plus) as series of z-stacks, 0.25 μM step, 512 × 512. Plan Apo λ 60x Oil objective with a numerical aperture of 1.4 was used.

Celauro L., Burato A., Zattoni M., De Cecco E., Fantuz M., Cazzaniga F.A., Bistaffa E., Moda F, & Legname G. (2023). Different tau fibril types reduce prion level in chronically and de novo infected cells. The Journal of Biological Chemistry, 299(8), 105054.

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Immunocytochemistry of Transfected HEK Cells

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As described in Section 2.3, the transfected HEK 293-T cells were obtained after a three-day culture. Cells were washed with phosphate-buffered saline (PBS) for three times and fixed with 1 mL/well 4% paraformaldehyde for 12 h at 4 °C. On a rotary shaker at 50 rpm, cells were immerged in tris buffered saline (TBS) containing 0.1% Triton X-100 (TBSx) for 3 min. Then, cells were subsequently incubated at 37 °C with TBSx containing 5% BSA and 1% serum from the rTgSIR2-vaccinated rat for 1.5 h on a rotary shaker at 50 rpm. After washing three times in TBSx, cells were then incubated at 37 °C in TBSx containing 5% BSA and 0.2% CY3-conjugated anti-rat IgG (Sigma, Saint Louis, MO, USA) on a rotary shaker at 50 rpm. After washing three times in TBSx again, 500 μL of 4′,6-diamidino-2-phenylindole (DAPI) staining solution (Beyotime, Shanghai, China) were added into each well and incubated at 37 °C on a rotary shaker at 50 rpm. Coverlips were mounted with 300 μL anti-fade mounting medium (Beyotime, Shanghai, China) after DAPI staining solution was completely removed. Cells were then immediately imaged by a laser scanning confocal microscopy (Nikon A1 plus, Nikon Corporation, Tokyo Metropolis, Japan).

Yu Z., Lu Y., Cao W., Aleem M.T., Liu J., Luo J., Yan R., Xu L., Song X, & Li X. (2021). Nano DNA Vaccine Encoding Toxoplasma gondii Histone Deacetylase SIR2 Enhanced Protective Immunity in Mice. Pharmaceutics, 13(10), 1582.

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Two-Photon Imaging of Vascular Pulsatility

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To visualize vasculature, 2 MDa FITC-dextran (50 mg/kg; Thermo Fisher Scientific) was injected via the tail vein. To tag amyloid plaques, methoxy-X04 (MX04, 5 mg/kg) was administered intraperitoneally 1 day before. The cortex was imaged using a two-photon microscope (Nikon A1 plus, Nikon Instruments) and a 25 × water immersion objective (NA 1.1, Nikon) was used throughout the experiment. The excitation wavelengths were 850 nm for vasculature and 800 nm for amyloid plaque. Angiography images were acquired at 512 × 512 pixels with 5-µm z-steps, and vascular pulsation was measured using a line scan. Vessels located within 150 µm of the cortical surface were selected for pulsatility index measurement. The scanning lines for the pulsatility index measurement were set orthogonal to the path of blood vessels. The scanning lines for penetrating arteries and ascending veins were chosen where the vascular cross-section appeared circular and located 50-150 µm below the surface. Body temperature was maintained at 37 °C through a feedback-controlled heating pad during the entire imaging session.

Kim S.H., Ahn J.H., Yang H., Lee P., Koh G.Y, & Jeong Y. (2020). Cerebral amyloid angiopathy aggravates perivascular clearance impairment in an Alzheimer’s disease mouse model. Acta Neuropathologica Communications, 8, 181.

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Correlative Light-Electron Microscopy

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CLEM was carried out at Japan Electron Optics Laboratory (Tokyo, Japan). Briefly, HeLa cells (cultured on a glass-bottom dish) that transiently expressed the iCMM system were washed twice with 1 ml of Phosphate buffer serin (PBS). The cells were then fixed for 10 min in a fixation mixture containing 4% formaldehyde (NEM, 3153) and 0.1% glutaraldehyde (NEM, 304) in PBS at room temperature. After three washes with PBS, the cells were imaged using a Nikon A1 plus equipped with an Apo λ S 40×C WI (Nikon), as well as a 405 and a 488 laser for ECFP and EYFP excitation, respectively. For SEM imaging, cells were subjected to post-fixation (1% OsO₄ and 1% tannic acid), Bloc contrast staining (1% uranyl acetate and lead aspartate), and dehydration, followed by Epon embedding. SEM imaging was carried out using a JSM-7900F (JEOL).

Miyamoto T., Uosaki H., Mizunoe Y., Han S.I., Goto S., Yamanaka D., Masuda M., Yoneyama Y., Nakamura H., Hattori N., Takeuchi Y., Ohno H., Sekiya M., Matsuzaka T., Hakuno F., Takahashi S.I., Yahagi N., Ito K, & Shimano H. (2021). Rapid manipulation of mitochondrial morphology in a living cell with iCMM. Cell Reports Methods, 1(4), 100052.

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Perfusion-Fixed Brain Sectioning and Immunostaining

Cited 2 times

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Mice of the appropriate age were anesthetized and perfused transcardially with 4% paraformaldehyde/PBS as previously described [10 (link), 12 (link), 14 (link)]. Brains were dissected, postfixed, and mounted in agarose prior to vibratome sectioning. Overall, 35–100 μm free-floating vibratome sections of brains were collected in PBS, blocked with 5% normal serum in PBS with 0.5% Triton X-100 and incubated with primary antibodies in blocking solution overnight at 4 °C. P14 vibratome sections were blocked with 5% normal serum in PBS with 0.1% Triton X-100 and 2% DMSO, and incubated with primary antibodies for 3 days. After 1–3 h incubation with Alexa Fluor-conjugated secondary antibodies (Invitrogen or Jackson Immunoresearch) followed by DAPI staining (0.1 μg/ml, Life Technologies), the stained slices were imaged using a confocal laser-scanning microscope (Zeiss LSM510, LSM710 or Nikon A1 plus).

Tang F.L., Zhao L., Zhao Y., Sun D., Zhu X.J., Mei L, & Xiong W.C. (2020). Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons. Cell Death and Differentiation, 27(7), 2099-2116.

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Immunofluorescence Assay for Bcl-2 and p53

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MCF-7 cells were seeded in 24-well plates. After different treatments, the cells were washed with PBS and then fixed in 4 % paraformaldehyde for 10 min at room temperature. The cells were then incubated overnight at 4 °C with rabbit monoclonal anti-bcl2 antibody or rabbit polyclonal anti-p53 antibody as primary antibodies. After this step, cells were washed with PBS three times and incubated for 1 h with FITC at 37 °C. After further washing with PBS, the cell nuclei were stained with DAPI. Staining cells without primary antibody utilized as negative. Images were captured using a laser confocal microscope (Nikon/A1 Plus, Japan).

Mirmalek S.A., Azizi M.A., Jangholi E., Yadollah-Damavandi S., Javidi M.A., Parsa Y., Parsa T., Salimi-Tabatabaee S.A., Ghasemzadeh kolagar H, & Alizadeh-Navaei R. (2016). Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line. Cancer Cell International, 16, 3.

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Multicolor Confocal Imaging of Fluorescent Fusion Proteins

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Image acquisition was performed using a confocal laser scanning microscope (Nikon A1 plus, Nikon, Japan) with a 40 × 1.15 NA water immersion objective. The BFP2, CFP, GFP, and RFP-containing fusion proteins were excited at λ 405 nm, 455 nm, 488 nm, and 561 nm, and emission was recorded in the range of 425–475 nm, 425–475 nm, 500–550 nm, and 570–620 nm, respectively. Pinholes were adjusted to 1 Airy unit for each wavelength. Post-acquisition image processing and analysis were performed using the software ImageJ (v.1.51, https://imagej.nih.gov/ij/index.html) and GraphPad Prism 8.0 (https://www.graphpad.com/scientific-software/prism).

Shao X., Xu H, & Pimpl P. (2023). Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling. Nature Communications, 14, 6926.

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