Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at − 80 °C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer’s instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9–2.1) using a NanoDrop OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scientific Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was confirmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer’s protocol of Reverse Transcription System.
Primmescript 2 1st strand cdna synthesis kit
Manufactured by Takara Bio
Sourced in China
The PrimmeScript™ II 1st strand cDNA Synthesis Kit is a tool for the synthesis of first-strand cDNA from RNA templates. It includes a reverse transcriptase enzyme and necessary reagents to facilitate the conversion of RNA into complementary DNA.
Automatically generated - may contain errors
3 protocols using primmescript 2 1st strand cdna synthesis kit
1
Total RNA Extraction from Cotton Plants
2
Transcriptome Analysis of Cotton Plant Organs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at -80°C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer's instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9-2.1) using a NanoDrop One C Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scienti c Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was con rmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer's protocol of Reverse Transcription System.
3
Transcriptome Analysis of Cotton Plant Organs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples from different organs of the cotton plants, including cotyledon, hypocotyl, petiole, leaf and stem of one or many different gland accessions, served as the source of total RNA, were immediately frozen in liquid nitrogen and stored at -80°C. Total RNAs were isolated from 100 mg of leaf ground with liquid nitrogen using the RNAprep Plant RNA kit (polysaccharides&polyphenolics-rich) (TIANGEN BIOTECH (BEIJING)CO., LTD) according to the manufacturer's instructions. The quantity and purity of RNAs were assessed according an absorbance ratio of OD 260/280 (1.9-2.1) using a NanoDrop One C Microvolume UV-Vis Spectrophotometer with Wi-Fi (Thermo Fisher Scienti c Inc., Waltham, MA, USA) ultraviolet spectrophotometer, and was con rmed using 1.0% (w/v) denatured formaldehyde agarose gel electrophoresis to investigate its integrality. First strand cDNA was synthesized by the PrimmeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa Bio, Dalian, China) following the manufacturer's protocol of Reverse Transcription System.
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