Sod activity assay kit

Manufactured by Solarbio

Sourced in China

The SOD Activity Assay Kit is a laboratory tool designed to measure the activity of the enzyme superoxide dismutase (SOD) in biological samples. It provides a quantitative assessment of SOD levels without making interpretations or extrapolations about its intended use.

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Lab products found in correlation

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14 protocols using sod activity assay kit

Oxidative Stress and Inflammatory Markers

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PRKs cell supernatant of each subgroup was collected to determine the levels of MDA, GSH, SOD, IL-6, IL-1β and TNF-α. The following kits were used according to the manufacturer's instructions: MDA Content Assay Kit (cat. no. BC0020; Beijing Solarbio Science & Technology Co., Ltd.), Reduced GSH Content Assay Kit (cat. no. BC1175; Beijing Solarbio Science & Technology Co., Ltd.), SOD Activity Assay Kit (cat. no. BC0170; Beijing Solarbio Science & Technology Co., Ltd.), Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit (cat. no. RLB00; R&D Systems), Rat IL-6 Quantikine ELISA kit (cat. no. R6000B; R&D Systems) and Rat TNF-α Quantikine ELISA Kit (cat. no. RTA00; R&D Systems).

Mai H., Huang Z., Zhang X., Zhang Y., Chen J., Chen M., Zhang Y., Song Y., Wang B., Lin Y, & Gu S. (2022). Protective effects of endothelial progenitor cell microvesicles carrying miR‑98‑5p on angiotensin II‑induced rat kidney cell injury. Experimental and Therapeutic Medicine, 24(5), 702.

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Oxidative Stress Biomarkers in OGD/R Cells

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The levels of SOD, MDA, GSH, and ATP were tested with SOD Activity Assay Kit (Solarbio), MDA Content Assay Kit (Solarbio), GSH Content Assay Kit (Solarbio), and ATP Content Assay Kit (Beyotime) respectively per manufacturer’s instructions. Cells suffered OGD/R process or cardiac tissues with different disposes were collected and carried out in accordance with the manufacturer’s protocol.

Ye T., Chen C., Wang D., Huang C., Yan Z., Chen Y., Jin X., Wang X., Ding X, & Shen C. (2024). Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury. Nature Communications, 15, 1682.

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Measuring Superoxide Dismutase Activity

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To detect the activity of SOD, at least 5×10⁶ cells in each group were collected after treatment. Then, SOD was extracted using an extracting solution from the SOD activity assay kit (BC0175, Solarbio, Beijing, China) (24 (link)). SOD activity was tested according to the protocol and assessed using absorbance at 560 nm with the Thermo 3001 microplate reader (Infinite 200 PRO, Tecan Austria GmbH, Salzburg, Austria). SOD activity was calculated based on the formula from the kit protocol. Relative SOD activity was calculated by normalizing the SOD activity in each group to that in the DMSO group. Each sample was measured at least in triplicate. Experiments were repeated in HGC-27 cells (n=8), MFC cells (n=6), and MKN-45 cells (n=5).

Ma J., Pan Z., Du H., Chen X., Zhu X., Hao W., Zheng Q, & Tang X. (2023). Luteolin induces apoptosis by impairing mitochondrial function and targeting the intrinsic apoptosis pathway in gastric cancer cells. Oncology Letters, 26(2), 327.

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PtsaN-C SOD-like Activity Assay

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The SOD-like activity was detected with SOD Activity Assay Kit (Solarbio) in accordance with the manufacturer’s instructions. Briefly, the working solution was incubated with PtsaN-C (5 µg/ml) or equal doses of references, then absorbance changes were monitored via UV-vis-NIR Epoch microplate reader at 560 nm.

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Serum SOD and MDA Quantification

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The collected serum was assayed for SOD and MDA expression levels according to the instructions of MDA Content Assay Kit (Solarbio, Beijing, China) and SOD Activity Assay Kit (Solarbio, Beijing, China), respectively.

Li X., Wei C., Jin Y., Zhang J., Zhong P., Zhang D, & Huang X. (2024). Time-resolved map of serum metabolome profiling in D-galactose-induced aging rats with exercise intervention. iScience, 27(2), 108999.

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Measuring Lung Tissue SOD Activity

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Right lung tissues from different treatment groups were collected, lung tissue homogenates were made with a homogenizer, and the SOD activity in lung tissues was measured with a SOD activity assay kit (Solarbio, BC0175) at a wavelength of 560 nm using an enzyme standardization instrument.

Tian Y., Deng Q., Yang X., Wang C., Minh Le V., Ji R., Liang X, & Feng Y. (2023). ISX-9 Promotes KGF Secretion From MSCs to Alleviate ALI Through NGFR-ERK-TAU-β-Catenin Signaling Axis. Stem Cells Translational Medicine, 13(3), 255-267.

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Cellular Oxidative Stress Analysis

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MDA level and SOD activity were determined to assess cell oxidative stress. The supernatant of WI-38 cells were collected for detecting MDA level and SOD activity using the MDA Assay Kit and SOD Activity Assay Kit (all from Solarbio) according to the kit instructions.

Yu Y., Yang T., Ding Z, & Cao Y. (2022). Circ_0026579 alleviates LPS-induced WI-38 cells inflammation injury in infantile pneumonia. Innate Immunity, 28(1), 37-48.

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Curcumin and Dopamine Nanoparticle Synthesis

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Curcumin (Cur) and dopamine hydrochloride (DA) all purchased from Aladdin. Maleimide-PEG2k-Fmoc (Fmoc-PEG-MAL) was purchased from Xi'an Ruixi Biological Technology (Xi'an, China). DMEM high glucose medium, trypsin, streptomycin, and glutamine were obtained from Hyclone. Fetal bovine serum (FBS) was supplied by Tianhang Biotech. ABTS reagent 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was sourced from Shanghai McLean Biochemical Technology Co., Ltd. Propidium iodide/acridine orange staining kit was provided by Biyuntian in China, Shanghai. MTT assay kit, MDA content detection kit, SOD activity assay kit, Prussian blue staining kit, and Fe²⁺ ion content detection kit were all procured from Solarbio Life Sciences. ROS detection assay kit, BCA protein concentration determination assay kit, and LPO assay kit were acquired from Biyuntian Biotech.

Lei L., Yuan J., Yang Q., Tu Q., Yu H., Chu L., Tang L, & Zhang C. (2024). Curcumin–polydopamine nanoparticles alleviate ferroptosis by iron chelation and inhibition of oxidative stress damage. RSC Advances, 14(21), 14934-14941.

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Evaluating Neuroprotective Factors in PC12 Cells

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The treated PC12 cell cultures were collected and processed according to an lactate dehydrogenase (LDH) assay kit instructions (Applygen, Beijing, China) and the OD of LDH in the cell cultures was measured and calculated for its activity. The cell culture medium was first centrifuged and the supernatant was discarded, then the lysis buffer was added and the cells were ultrasonically crushed under the premise of ice bath. The supernatant was then separated by centrifugation at 8000 g for 10 min at 4°C, separated in a water bath at 37°C for 30 min, and the absorbance was measured at 560 nm.
The superoxide dismutase (SOD) activity of the cells was calculated according to a SOD activity assay kit (Solarbio, Beijing, China). PC12 cell cultures were taken and the cells were resuspended with DCFH-DA at a final concentration of 10 μmol/L. The cells were incubated for 20 min at 37°C, three times washed with serum-free medium, and intracellular reactive oxygen species (ROS) levels were

Zhang X., Bao M., Zhang J., Zhu L., Wang D., Liu X., Xu L., Luan L., Liu Y, & Liu Y. (2024). Neuroprotective mechanism of ribisin A on H₂O₂-induced PC12 cell injury model. Tissue & cell, 87.

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Enzymatic Activity Quantification

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Measurement of peroxidase (POD) activity was performed by POD Activity Assay Kit (Solarbio, Beijing, China). POD activity was articulated as U/g. Measurement of superoxide dismutase (SOD) activity was performed by SOD Activity Assay Kit (Solarbio, Beijing, China). SOD activity was articulated as U/g. Measurement of catalase (CAT) activity was performed by CAT Activity Assay Kit (Solarbio, Beijing, China). CAT activity was articulated as U/g.

Fang R., Lv G., Zhang X., Chen J., Chen X, & Wang B. (2023). Preharvest 24-epibrassinolide treatment prolongs harvest duration and shelf life in sweet corn. Food Chemistry: Molecular Sciences, 7, 100179.

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