Nano-graphene oxide (nGO) was prepared according to our previous work [28 (link),33 (link),34 (link)]. Briefly, starch was carbonized in dilute sulfuric acid by heating in a microwave oven (Milestone UltraWAVE, Milestone Inc., Sorisole, Italy) for 2 h at 180 °C. The obtained carbon spheres were further oxidized in nitric acid for 30 min at 90 °C. nGO was obtained after rotary evaporation of the diluted acidic solution followed by freeze-drying. PLA and PCL were dissolved in DCM/DMF (4/6 w/w) at 10% w/w concentration. Different amounts of nGO were added to the PLA or PCL solutions to prepare 0%, 1%, 2.5%, and 5% (nGO/P, w/w, where P stands for PLA and PCL) solutions. Electrospinning was performed on these solutions to fabricate PLA and PCL fibers with four different nGO concentrations. The fibers were denoted as P fiber, P-nGO1 fiber, P-nGO2.5 fiber, and P-nGO5 fiber, respectively (P stands for PLA or PCL). Each solution was filled in a 2 mL plastic syringe and electrospun at 20 cm distance from the collector, under a flow rate of 0.5 mL/h and a voltage of 23 kV. Fibers were collected on both aluminum foils and glass covers.
Ultrawave
Manufactured by Milestone
Sourced in Italy, United States
The UltraWAVE is a laboratory equipment designed for sample preparation. It utilizes microwave technology to facilitate efficient and controlled heating of samples for various analytical processes.
Automatically generated - may contain errors
Lab products found in correlation
S10 autosampler, by PerkinElmer (2 mentions)
Glass cyclonic spray chamber, by PerkinElmer (2 mentions)
Icap rq, by Thermo Fisher Scientific (2 mentions)
Winlab32, by PerkinElmer (2 mentions)
Icp oes, by Agilent Technologies (1 mentions)
Nexion 300x, by PerkinElmer (1 mentions)
Agilent 5100, by Agilent Technologies (1 mentions)
Icp oes 5110 vdv, by Agilent Technologies (1 mentions)
Epson1680, by Regent Instruments (1 mentions)
Winrhizo pro2003b, by Regent Instruments (1 mentions)
Ultrapur hno3, by Kanto Chemical (1 mentions)
5100 icp oes, by Agilent Technologies (1 mentions)
Sncl2, by Scharlab (1 mentions)
Flash ea 1112, by Thermo Fisher Scientific (1 mentions)
Nexion 300, by PerkinElmer (1 mentions)
Chn irms isoprime 100 stable isotope ratio mass spectrometer, by Elementar (1 mentions)
Nexion 300x icp ms, by PerkinElmer (1 mentions)
Cary 60, by Agilent Technologies (1 mentions)
Vacuum centrifuge, by Eppendorf (1 mentions)
Nexion 300d, by PerkinElmer (1 mentions)
55 protocols using ultrawave
1
Fabrication of Nano-Graphene Oxide Reinforced Polymer Fibers
2
Determination of Bone Mineral Content
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the determination of bone mineral contents, predominantly Ca (%) and P (%) in the tibia bone, 500 mg of ash were homogenized and digested in the Milestone Ultrawave® microwave (Milestone Srl, Sorisole, Italy) with nitric acid (HNO3) and then the concentration of minerals was measured by inductively coupled plasma emission spectrometer (ICP-OES) (Agilent, Santa Clara, CA, USA). In brief, 500 mg of ash sample were taken into a Teflon tube and added with 1 ml of deionised water and 4 ml of 70% HNO3 solution for pre-digestion overnight. Then the Teflon tubes were loaded in a digestion rack and placed in the microwave cavity following the Ultrawave instructions for digestion time and pressure. After completion of the digestion process, the digested samples were made to approximately 25 ml total volume with deionised water for dilution giving a 16% HNO3 concentration. Each sample was run in triplicate and the average was taken across the three runs to determine mineral contents. Standard and blank samples were also run for quality assurance.
3
Nutrient Digestibility Analysis of Animal Feeds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For nutrient digestibility analysis, crude protein, crude fat, DM, crude ash, and mineral concentrations of starter, grower and finisher diets as well as from lyophilised digesta were determined. Crude protein, crude fat and DM analysis were performed as per the methods described in section 2.2 . Crude ash of digesta was determined following the AOAC Official method number 942.05 (AOAC, 2005 ). For mineral determination, ash samples were used to quantify calcium and phosphorous using the ultrawave microwave digestion system (Milestone Ultra WAVE, Milestone Srl, Sorisole, Italy). In short, roughly 0.2 g of homogenised ash sample was digested in 4 mL of concentrated nitric acid using the single reactor chamber of the microwave in a temperature range of 110 to 240 °C and a pressure of up to 110 bar. The digested samples were transferred to a 25 mL container with deionized water. The analysis for calcium and phosphorus was carried out using an inductively coupled plasma optical emission spectrometer (ICP-OES; Agilent Australia, Victoria, Australia) at wavelengths 422.673 nm for calcium and 213.618 nm for phosphorus.
4
Quantification of Selenium in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the quantification of selenium (Se), the protocol proposed by López-Bellido et al. [18 (link)] was followed, with some modifications. Briefly, the dry samples (approximately 0.5 g) were pretreated by acid digestion, using HNO3 in a microwave (UltraWAVE Milestone, Shelton, CT, USA). Then, the samples were diluted 1:2 (w/v) with 1% nitric acid and analyzed by inductively coupled plasma mass spectrometry (ICP-MS model NexION 300 X, Perkin Elmer®, Waltham, MA, USA) under the following operating conditions: nebulizer quartz concentric, spray chamber PC3 Peltier Cooled Cyclonic, RF Power 1500 W, nebulizer gas flow of 0.86 L/min, scanning mode in peak hopping, d.w.ell time of 50 ms, 20 sweeps, 1 reading, 3 replicates, analytes 78Se and 80Se, internal standard 72Ge, and reaction mode CH4 = 0.70 mL/min and RPq = 0.55. Calibration standards at 0.05, 0.1, 0.5, 1, 5, 10, and 50 μg/L were prepared from a 1000 mg/L stock solution of Se (SCP Science) in 1% nitric acid. One internal standard (Ge at 30 μg/L) was used to correct for signal drift. The results were expressed as µg/100 g d.w. or f.w.
5
Microwave-Assisted Elemental Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Substrates were digested in nitric acid and hydrogen peroxide at 240°C and 200 bars in a pressurized microwave oven for 15 minutes (Ultrawave, Milestone Inc., Italy). Hydrofluoric acid was added after digestion and cooling, and the samples were left standing overnight. The solutions were diluted before final analysis by inductively coupled plasma optical emission spectrometry (ICP-OES,Agilent 5100, Agilent Technologies, Santa Clara, USA)), using a procedure described by Hansen et al. (2009) (Hansen et al., 2009) .
6
Phospholipid Mineralization and ICP-OES Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Few microliters (5–10) of phospholipids solutions were dispersed in 1 mL of distilled water and added with 1 mL of concentrated nitric acid (HNO3, 68%). The acid dispersions of the phospholipids were then completely mineralized at high temperature by means of a microwave digestion system (UltraWave, Milestone Srl, Italy) using a two-step heating program (25 min from room temperature up to 240 °C, then held 15 min at 240 °C). After mineralization, the solutions were diluted with distilled water to the final volume of 4 mL, filtered (0.45 μm), and analyzed by ICP-OES 5110 VDV (Agilent Technologies Italia, SpA, Italy). The emission lines used were 177.434 nm and 213.618 nm. The P concentration was determined by using a 5-point (0.05–0.1-0.2–0.5–1.0 ppm) calibration curve, obtained from certified single element standard solutions (Agilent Technologies Italia, SpA, Italy).
7
Root Morphology and Copper Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At harvest, root morphology parameters (total root length, number of tips, root volume, and average root diameter) were determined using the Winrhizo software (EPSON1680, WinRHIZO Pro2003b, Regent Instruments Inc., Quebec, Canada). Afterward, the roots and leaves were separated and dried at 65°C until constant weight was reached, a portion of the root apparatus was frozen in liquid nitrogen and kept at −80°C for gene expression analysis. Roots and leaves tissues were acid digested (HNO3 65% v/v) in a single digestion chamber (SRC) microwave digestion system (UltraWAVE, Milestone, Shelton, CT, United States) and the Cu concentration was determined by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES, Arcos Ametek Spectro, Germany).
8
Metrological Traceability of Rb and Sr
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Metrological traceable single-element standard solutions for Rb and Sr were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). The traceability of the standard solutions were guaranteed by the Japan Calibration Service System (JCSS). Ultrapur ® HNO3 was also purchased from Kanto Chemical Co., Inc., and used for preparing solutions in the present experiment. High-purity gases (purity >99.0%) of CH3F and CD3F were purchased from TOMOE SHOKAI Co., Ltd. (Tokyo, Japan).
CRM samples were randomly drawn from the storage for research purposes. Solid samples were transformed to solution samples by microwave-assisted acid digestion using an UltraWAVE instrument (Milestone Inc., Shelton, USA).
CRM samples were randomly drawn from the storage for research purposes. Solid samples were transformed to solution samples by microwave-assisted acid digestion using an UltraWAVE instrument (Milestone Inc., Shelton, USA).
9
Microwave Digestion for Calcium Content
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Snack samples, WPI, WPH and WMC were all microwaved (UltraWAVE, Milestone Srl, Sorisole, Italy) prior to analysis to ensure that all organic matter was digested. Briefly, 100 mg samples were digested at 240 °C with 2.5 mL of 70% HNO3 and 1.0 mL of 15% H2O2 with Milli-Q water to 50 mL. Total calcium content was analyzed in triplicate by ion coupled plasma optical emission spectroscopy (5100 ICP-OES, Agilent Technologies, Santa Clara, CA, USA) at the wavelengths of 318.1 nm.
10
Determining Hg and Se in Biota
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Freeze-dried and homogenized tissue samples (0.05-0.2 g) corresponding to the bulk and to the water-soluble fraction (details in the section below) were digested (in triplicate) with 1.5 mL of HNO3 (ca. 63%, analytical grade) after overnight pre-digestion at room temperature, in an Ultrawave, Milestone Srl system (Table S2). For Se analyses, the resulting solutions were appropriately diluted with Milli-Q water and analysed by ICP-MS according to the conditions given in Table S3. The measurement of total Hg (THg) in the digests was performed by atomic fluorescence with previous Cold Vapor Generation of Hg(0) by reduction with SnCl2 3% (Scharlab, Barcelona, Spain) in 10% HCl (J.T.Baker, Fisher scientific, Illkirch, France) in Milli-Q water. The samples were then diluted in HCl 5% for analyses and the quantification was performed by standard calibration. Measurements were validated using certified reference materials (DOLT-5, CE-101, BCR-464 and TORT-2). The information relative to the quality assurance of the analyses of Hg and Se is found in Table S4. Concentrations of both elements are further reported in µg g -1 dw.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!