MiRNA-mimics and miRNA-inhibitors (sequences shown in Table 1 ) were synthesized (Gene Pharma) in the form of small interfering RNA duplexes or single strands according to Park’s study (Park et al., 2009). SH-SY5Y cells in each 6-well plate were transfected with 150ng miRNA and 1.5mL of Hepfect reagent according to the manufacturer’s instructions (Qiagen, Germany). SH-SY5Y cell line was chosen, because SH-SY5Y cells express human DRD2 mRNA and protein.
Mirna mimics and mirna inhibitors
Manufactured by GenePharma
Sourced in United States
MiRNA-mimics and miRNA-inhibitors are synthetic oligonucleotides designed to modulate the expression of specific microRNA (miRNA) molecules. MiRNA-mimics are used to increase the activity of a target miRNA, while miRNA-inhibitors are used to decrease the activity of a target miRNA. These products are intended for use in research applications to study the biological functions of miRNAs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mirna mimics and mirna inhibitors
1
Transfecting miRNA Mimics and Inhibitors in SH-SY5Y Cells
2
Investigating CircLDLR in Colorectal Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human CRC cell lines were cultured in a 6-well plate at 37 °C in a humidified 5% CO2 atmosphere overnight. CircLDLR-specific siRNA (siRNA-1, -2, -3, -4 and -5), miRNA mimics and miRNA inhibitors (GenePharma) were transfected with Lipofectamine 2000 (Invitrogen, USA) in the light of the manufacturer’s protocol.
Lentiviruses carrying a circLDLR overexpression vector or short hairpin RNA (shRNA) containing the sequence of circLDLR siRNA-2 was obtained from Geneseed (Guangzhou, China). We used an empty backbone vector as a control. When CRC cells grew to 30% confluence, lentiviral particles (MOI: 20) were used to infect them. We verified the effectiveness of overexpression or interference by fluorescence microscopy and qRT-PCR after 72 h.
Lentiviruses carrying a circLDLR overexpression vector or short hairpin RNA (shRNA) containing the sequence of circLDLR siRNA-2 was obtained from Geneseed (Guangzhou, China). We used an empty backbone vector as a control. When CRC cells grew to 30% confluence, lentiviral particles (MOI: 20) were used to infect them. We verified the effectiveness of overexpression or interference by fluorescence microscopy and qRT-PCR after 72 h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!