One μg of total RNA was reverse-transcribed into cDNA using the RT2 First Strand Kit (Qiagen). Custom RT2 Profiler PCR array plates including controls for human genomic DNA contamination, reverse transcription and PCR efficacy (SABiosciences, Qiagen) were used for RQ–PCR reactions performed using RT2 SYBR Green ROX qPCR Mastermix (SABiosciences, Valencia, CA, USA) on an ABI 7900 thermal cycler (Applied Biosystems, Foster City, CA, USA) as described previously.47 (link)
Custom rt2 profiler pcr array plates
Manufactured by Qiagen
Sourced in United States
The Custom RT2 Profiler PCR array plates are a versatile tool for gene expression analysis. These plates allow for the customized assessment of specific gene sets, enabling researchers to focus their investigations on pathways or processes of interest. The plates provide a standardized format for real-time PCR (RT-PCR) experiments, facilitating efficient and reliable data collection.
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2 protocols using custom rt2 profiler pcr array plates
1
Quantitative real-time RT-PCR for gene expression analysis
2
Profiling Gene Expression via RT-PCR
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Custom RT2 Profiler PCR Array plates containing the 14 genes focused in the study and ribosomal protein, large, P1 (Rplp1) as the internal control were ordered and obtained from SABiosciences (MD, USA). Quantitative real-time PCR was performed using Applied Biosystems 7700 sequence detection system under conditions suggested by SABiosciences Co. for 96-well array plate.
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