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3h cocaine 50 ci mmol

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[3H](−)-Cocaine (50 Ci/mmol) is a radioactive labelled compound with a specific activity of 50 Ci/mmol. This product is intended for research use only.

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Lab products found in correlation

Freestyle cho expression medium, by Thermo Fisher Scientific (4 mentions) Transit pro transfection kit, by Mirus Bio (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Simpleblue safestain, by Thermo Fisher Scientific (2 mentions) Tris glycine mini protein gel, by Thermo Fisher Scientific (2 mentions) Rmp protein a sepharose fast flow, by GE Healthcare (2 mentions) Pcmv mcs, by Agilent Technologies (2 mentions) T4 dna ligase, by New England Biolabs (2 mentions) Chinese hamster ovary cho s cells, by Thermo Fisher Scientific (2 mentions) Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions) Dpni endonuclease, by New England Biolabs (1 mentions) Phusion dna polymerase, by New England Biolabs (1 mentions) Restriction endonuclease, by New England Biolabs (1 mentions) Q5 hot start high fidelity dna polymerase, by New England Biolabs (1 mentions) Gibson assembly master mix, by New England Biolabs (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Amicon ultra 15 centrifugal filter unit, by Merck Group (1 mentions) Restriction enzyme, by New England Biolabs (1 mentions) Qiaprep spin plasmid miniprep kit, by Qiagen (1 mentions) Alkaline phosphatase cip, by New England Biolabs (1 mentions)

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[3H](−)-Cocaine (10 citations)

4 protocols using 3h cocaine 50 ci mmol

1

Recombinant Protein Expression in CHO Cells

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Q5® Site-Directed Mutagenesis Kit was ordered from New England Biolabs (Ipswich, MA). All oligonucleotides were synthesized by Eurofins MWG Operon (Huntsville, AL). Chinese Hamster Ovary-suspension (CHO-S) cells, FreeStyle™ CHO Expression Medium, Fetal Bovine Serum (FBS), 4-12% Tris-Glycine Mini Protein Gel, and SimpleBlue SafeStain were obtained from Invitrogen (Grand Island, NY). TransIT-PRO® Transfection Kit was purchased from Mirus (Madison, WI). The rmp Protein A Sepharose Fast Flow was from GE Healthcare Life Sciences (Pittsburgh, PA). (–)-Cocaine was provided by the National Institute on Drug Abuse (NIDA) Drug Supply Program (Bethesda, MD); and [3H](–)-Cocaine (50 Ci/mmol) was obtained from PerkinElmer (Waltham, Massachusetts). All other materials were from Sigma-Aldrich (St Louis, MO) or Thermo Fisher Scientific (Waltham, MA).
Chen X., Deng J., Zheng X., Zhang J., Zhou Z., Wei H., Zhan C.G, & Zheng F. (2019). Development of a Long-acting Fc-fused Cocaine Hydrolase with Improved Yield of Protein Expression. Chemico-biological interactions, 306, 89-95.
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2

Recombinant Cocaine Receptor Expression

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Expression vector pCMV-MCS was obtained from Agilent (Santa Clara, CA). Phusion DNA polymerase, restriction endonucleases, T4 DNA ligase, and DpnI endonuclease were ordered from New England Biolabs (Ipswich, MA). All oligonucleotides were synthesized by Eurofins MWG Operon (Huntsville, AL). Chinese Hamster Ovary-suspension (CHO-S) cells, FreeStyle™ CHO Expression Medium, Fetal Bovine Serum (FBS), 4–12% Tris-Glycine Mini Protein Gel, and SimpleBlue SafeStain were obtained from Invitrogen (Grand Island, NY). TransIT-PRO® Transfection Kit was purchased from Mirus (Madison, WI). The rmp Protein A Sepharose Fast Flow was from GE Healthcare Life Sciences (Pittsburgh, PA). (−)-Cocaine was provided by the National Institute on Drug Abuse (NIDA) Drug Supply Program (Bethesda, MD); and [3H](−)-Cocaine (50 Ci/mmol) was obtained from PerkinElmer (Waltham, Massachusetts). All of other materials were from Sigma-Aldrich (St Louis, MO) or Thermo Fisher Scientific (Waltham, MA).
Chen X., Deng J., Cui W., Hou S., Zhang J., Zheng X., Ding X., Wei H., Zhou Z., Kim K., Zhan C.G, & Zheng F. (2018). Development of Fc-fused Cocaine Hydrolase for Cocaine Addiction Treatment: Catalytic and Pharmacokinetic Properties. The AAPS journal, 20(3), 53.
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3

High-Yield Expression and Purification of Cocaine Receptor

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Expression vector pCMV-MCS was obtained from Agilent (Santa Clara, CA). Q5 hot start high-fidelity DNA polymerase, Kinase-Ligase-DpnI (KLD) enzyme mix, and Gibson Assembly® Master Mix were ordered from New England Biolabs (Ipswich, MA). All oligonucleotides were synthesized by Eurofins MWG Operon (Huntsville, AL). Chinese Hamster Ovary (CHO-S) cells, FreeStyle™ CHO Expression Medium, fetal bovine serum (FBS), hypoxanthine/thymidine (HT) supplement, and L-glutamine were obtained from Invitrogen (Grand Island, NY). TransIT-PRO® Transfection Kit was purchased from Mirus (Madison, WI). The AlbuPure® was from Prometic Life Science (Québec, Canada), and Amicon Ultra-15 Centrifugal Filter Unit was from MilliporeSigma (Massachusetts, United States). (−)-Cocaine was provided by the National Institute on Drug Abuse (NIDA) Drug Supply Program (Bethesda, MD), and [3H](−)-Cocaine (50 Ci/mmol) was obtained from PerkinElmer (Waltham, Massachusetts). All of the other materials were from Sigma-Aldrich (St Louis, MO) or Thermo Fisher Scientific (Waltham, MA).
Cai Y., Zhou S., Jin Z., Wei H., Shang L., Deng J., Zhan C.G, & Zheng F. (2019). Reengineering of albumin-fused cocaine hydrolase CocH1 (TV-1380) to prolong its biological half-life. The AAPS journal, 22(1), 5.
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4

Cloning and Expression of mBChE

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The cDNA for mBChE containing N-terminal signal was kindly provided by Dr. Palmer Taylor (Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, CA). Cloned pfu DNA polymerase and Dpn I endonuclease were obtained from Stratagene (La Jolla, CA). Restriction enzyme, alkaline phosphatase (CIP), and T4 DNA ligase were purchased from New England Biolabs (Ipswich, MA). All oligonucleotides were synthesized by Eurofins MWG Operon (Huntsville, AL). Vector pCMV-MCS was obtained from Agilent Technologies (Santa Clara, CA). The QIAprep Spin Plasmid Miniprep Kit and QIAquick Gel Extraction Kit were obtained from QIAGEN (Valencia, CA). Chinese hamster ovary (CHO)-S cells and FreeStyle™ CHO Expression Medium were ordered from Invitrogen (Grand Island, NY). [3H](−)-Cocaine (50 Ci/mmol) and [3H]acetylcholine (ACh) were purchased from PerkinElmer Life and Analytical Sciences (Waltham, MA). Butyrylthiocholine (BTC) was obtained from Sigma-Aldrich (St Louis, MO).
Chen X., Huang X., Geng L., Xue L., Hou S., Zheng X., Brimijoin S., Zheng F, & Zhan C.G. (2015). Kinetic Characterization of a Cocaine Hydrolase Engineered from Mouse Butyrylcholinesterase. The Biochemical journal, 466(2), 243-251.
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