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Agilent tapestation high sensitivity d1000 assay

Manufactured by Agilent Technologies
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The Agilent TapeStation High Sensitivity D1000 Assay is a lab equipment product designed for the analysis of DNA samples. It provides rapid and accurate size-based separation and quantification of DNA fragments ranging from 35 to 1,000 base pairs. The assay utilizes a microfluidic-based platform to automate the sample preparation and analysis process.

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Lab products found in correlation

Sureselectxt human all exon v5, by Agilent Technologies (2 mentions) Kapa hyper prep kit, by Roche (2 mentions) Kapa hyperprep kit, by Covaris (1 mentions)

2 protocols using agilent tapestation high sensitivity d1000 assay

1

FFPE DNA Library Preparation and Enrichment

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For DNA extracted from Formalin-fixed paraffin embedded (FFPE) tissue, adapter-ligated libraries were prepared using the KAPA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA) followed by Agilent SureSelectXT capture enrichment according to the manufacturers’ protocols. Samples were normalised to 400 ng and sheared to 150–200 bp using a Covaris E220 (Covaris, Woburn, MA, USA), following the parameters outlined in the KAPA HyperPrep Kit for SureSelect Target Enrichment protocol. KAPA HyperPrep libraries were generated and amplified using 10, 11 or 12 pre-capture PCR cycles and subsequently enriched using either the Agilent custom Melanoma Driver Panel or SureSelectXT Human All Exon v5 capture library. The quality and fragment size distributions of the purified libraries were assessed using the Agilent TapeStation High Sensitivity D1000 Assay (Agilent Technologies).
Spain L., Coulton A., Lobon I., Rowan A., Schnidrig D., Shepherd S.T., Shum B., Byrne F., Goicoechea M., Piperni E., Au L., Edmonds K., Carlyle E., Hunter N., Renn A., Messiou C., Hughes P., Nobbs J., Foijer F., van den Bos H., Wardenaar R., Spierings D.C., Spencer C., Schmitt A.M., Tippu Z., Lingard K., Grostate L., Peat K., Kelly K., Sarker S., Vaughan S., Mangwende M., Terry L., Kelly D., Biano J., Murra A., Korteweg J., Lewis C., O'Flaherty M., Cattin A.L., Emmerich M., Gerard C.L., Pallikonda H.A., Lynch J., Mason R., Rogiers A., Xu H., Huebner A., McGranahan N., Al Bakir M., Murai J., Naceur-Lombardelli C., Borg E., Mitchison M., Moore D.A., Falzon M., Proctor I., Stamp G.W., Nye E.L., Young K., Furness A.J., Pickering L., Stewart R., Mahadeva U., Green A., Larkin J., Litchfield K., Swanton C., Jamal-Hanjani M, & Turajlic S. (2023). Late-Stage Metastatic Melanoma Emerges through a Diversity of Evolutionary Pathways. Cancer Discovery, 13(6), 1364-1385.
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2

FFPE DNA Capture Enrichment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For DNA extracted from FFPE tissue, adapter-ligated libraries were prepared using the KAPA HyperPrep Kit (KAPA Biosystems), followed by Agilent SureSelectXT capture enrichment according to the manufacturer's protocols. Samples were normalized to 400 ng and sheared to 150 to 200 bp using a Covaris E220 (Covaris), following the parameters outlined in the KAPA HyperPrep Kit for SureSelect Target Enrichment protocol. KAPA HyperPrep libraries were generated and amplified using 10, 11, or 12 precapture PCR cycles and subsequently enriched using either the Agilent custom Melanoma Driver Panel or SureSelectXT Human All Exon v5 capture library. The quality and fragment size distributions of the purified libraries were assessed using the Agilent TapeStation High-Sensitivity D1000 Assay (Agilent Technologies).
Spain L., Coulton A., Lobon I., Rowan A., Schnidrig D., Shepherd S.T., Shum B., Byrne F., Goicoechea M., Piperni E., Au L., Edmonds K., Carlyle E., Hunter N., Renn A., Messiou C., Hughes P., Nobbs J., Foijer F., van den Bos H., Wardenaar R., Spierings D.C., Spencer C., Schmitt A.M., Tippu Z., Lingard K., Grostate L., Peat K., Kelly K., Sarker S., Vaughan S., Mangwende M., Terry L., Kelly D., Biano J., Murra A., Korteweg J., Lewis C., O'Flaherty M., Cattin A.L., Emmerich M., Gerard C.L., Pallikonda H.A., Lynch J., Mason R., Rogiers A., Xu H., Huebner A., McGranahan N., Al Bakir M., Murai J., Naceur-Lombardelli C., Borg E., Mitchison M., Moore D.A., Falzon M., Proctor I., Stamp G.W., Nye E.L., Young K., Furness A.J., Pickering L., Stewart R., Mahadeva U., Green A., Larkin J., Litchfield K., Swanton C., Jamal-Hanjani M, & Turajlic S. (2023). Late-Stage Metastatic Melanoma Emerges through a Diversity of Evolutionary Pathways. Cancer Discovery, 13(6), 1364-1385.
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