Recombinant human il 6 receptor

Dynabeads Human T-Activator CD3/28 was purchased from Invitrogen (Carlsbad, CA, USA). An affiniPure F (ab’)² Fragment Goat Anti-Human IgA/IgG/IgM (H+L) (anti-BCR, 10 μg/ml) was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Recombinant human cytokines (IL-12 (50 ng/ml), IFN-γ (20 ng/ml), IL-4 (20 ng/ml), IL-6 (50 ng/ml), IL-10 (10 ng/ml), IL-21 (50 ng/ml), TGF-β (50 ng/ml), IL-2 (100 U/ml), IL-15 (1 ng/ml) and recombinant human IL-6 Receptor (100 ng/ml) were obtained from R&D Systems (Minneapolis, MN, USA). CpG oligonucleotide type B (0.1 μM) was purchased from Gene Design Inc. (Osaka, Japan). Phorbol myristate acetate (PMA) and Ionomycin were purchased from Calbiochem (Nottingham, UK). A human monoclonal antibody (mAb) against IL-21 (anti-IL-21, used at the dose of 10 μg/ml) was purchased from Mabtech AB (Nacka Strand, Sweden).

An affiniPure F (ab’) Fragment Goat Anti-Human IgA/IgG/IgM (H + L) (BCR, 10 μg/ml) was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Anti-human CD40 monoclonal antibody (CD40, 2 μg/ml), recombinant human cytokines (TNF-α (100 ng/ml), IFN-γ (20 ng/ml), IL-4 (20 ng/ml), IL-17 (100 ng/ml), IL-2 (100 ng/ml), IL-6 (100 ng/ml)), recombinant human IL-6 Receptor (100 ng/ml) and OPG (100 ng/ml)) were from R&D Systems (Minneapolis, MN, USA). Recombinant human CXC motif chemokine ligand 10 (CXCL10, 100 ng/ml) was from PeproTech Inc (Rocky Hill, NJ, USA). Recombinant human IL-21(20 ng/ml) was from Miltenyi Biotec (Auburn, CA, USA). CpG ODN 2006, type C (Toll-like receptor 9 (TLR9), 0.1 μM) was from Gene Design Inc (Osaka, Japan). A fully human monoclonal antibody against RANKL (αRANKL, 100 ng/ml) was from Amgen Inc (Thousand Oaks, CA, USA).