Anti usp21

Manufactured by Thermo Fisher Scientific

Anti-USP21 is a lab equipment product offered by Thermo Fisher Scientific. It functions as an inhibitor of the deubiquitinating enzyme USP21. The core purpose of this product is to facilitate the study and understanding of the biological role of USP21 in various cellular processes.

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Lab products found in correlation

Anti stat3, by Cell Signaling Technology (2 mentions) Anti foxd1, by Thermo Fisher Scientific (2 mentions) Anti cd44, by Cell Signaling Technology (2 mentions) Anti aldh1a3, by Abcam (2 mentions) Mg132, by Merck Group (1 mentions) Anti myc, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Mouse igg isotype control, by Cell Signaling Technology (1 mentions) Anti phospho stat3, by Cell Signaling Technology (1 mentions) Rabbit igg isotype control, by Cell Signaling Technology (1 mentions) Ab104139, by Abcam (1 mentions) Ab150113, by Abcam (1 mentions) Ab150079, by Abcam (1 mentions) Gapdh, by Abcam (1 mentions) Cell lysis buffer, by Beyotime (1 mentions) Anti p stat3, by Cell Signaling Technology (1 mentions) Anti gst, by Cell Signaling Technology (1 mentions) Anti c met, by Abcam (1 mentions) Anti ha, by Abcam (1 mentions) Anti c ebpβ, by Abcam (1 mentions)

3 protocols using anti usp21

Comprehensive Immunoblotting Protocol

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The following reagents and antibodies were used in this study:
MG132 (Sigma-Aldrich, Cat#M7449); anti-Flag (Sigma-Aldrich, Cat#F7425); anti-MYC (Sigma-Aldrich, Cat#M4439); anti-STAT3 (Cell Signaling Technology, Cat#9319S); anti-phospho-STAT3 (Cell Signaling Technology, Cat#9145S).
Mouse IgG Isotype control (Cell Signaling Technology, Cat#53484).
Rabbit IgG Isotype control (Cell Signaling Technology, Cat#2729); anti-Usp21 (Invitrogen, Cat#PA5-110556); anti-beta actin (2D4H5) (Proteintech, Cat#66 009-1-Ig).

Deng B., Yang B., Chen J., Wang S., Zhang W., Guo Y., Han Y., Li H., Dang Y., Yuan Y., Dai X., Zang Y., Li Y, & Li B. (2022). Gallic acid induces T-helper-1-like Treg cells and strengthens immune checkpoint blockade efficacy. Journal for Immunotherapy of Cancer, 10(7), e004037.

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Immunofluorescence and Immunohistochemistry Analysis of GSCs

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Following antibodies were used for IF and IHC analysis. Anti-USP21 (catalog MA5-34953), anti-FOXD1 (catalog PA5-35145) were purchased from Invitrogen. Anti-ALDH1A3 (catalog ab129815) was purchased from Abcam. Anti-CD44 (catalog 3570) was purchased from Cell Signaling Technology.
GSCs were fixed in 4% formaldehyde for IF staining, permeated with 0.25% Triton X-100, and then blocked with 1% BSA at room temperature for 1 h. The cells were probed with the primary antibody. After washing by PBS-T, cells were combined with the Alexa Fluor 488 (abcam, catalog ab150113) or Alexa Fluor 647 (abcam, catalog ab150079) labeled secondary antibody.We used a mounting medium containing DAPI (abcam, catalog ab104139). The samples were observed through a confocal laser scanning microscope. For IHC staining, the slides were handled as before [29 (link)]. If <10% of the cells in the tumor area were stained, the result would be classified as negative. If the staining were 10% to 100%, the result would be positive. The percentage of positive tumor cells per slide (10–100%) multiplied by the main intensity pattern of staining (1, weak; 2, medium; 3, strong).

Zhang Q., Chen Z., Tang Q., Wang Z., Lu J., You Y, & Wang H. (2022). USP21 promotes self-renewal and tumorigenicity of mesenchymal glioblastoma stem cells by deubiquitinating and stabilizing FOXD1. Cell Death & Disease, 13(8), 712.

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Western Blot Analysis of Diverse Cellular Proteins

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Following antibodies were used for IB analysis. Anti-USP21 (catalog MA5-34953), anti-FOXD1 (catalog PA5-27142) were purchased from Invitrogen. Anti-TAZ (catalog ab84927), anti-C/EBPβ (catalog ab32358), anti-c-MET (catalog ab74217), anti-ALDH1A3 (catalog ab129815), anti-OLIG2 (catalog ab109186), anti-Flag (catalog ab205606), anti-Myc (catalog ab32), anti-HA (catalog 236632), and GAPDH (catalog ab8245) were purchased from Abcam. Anti-CD44 (catalog 3570), anti-p-STAT3 (catalog 9145), anti-STAT3 (catalog 9139), anti-mouse IgG (catalog 5415), anti-rabbit IgG (catalog 3900), and anti-GST (catalog 2624) were purchased from Cell Signaling Technology.
Samples or cells with indicated treatments were lysed with cell lysis buffer (Beyotime, catalog P0013) with protease Cocktail Inhibitor (MCE, catalog HY-K0010) at 4 °C for 30 minutes. The lysates were centrifugated at 12,000 rpm for 20 min. Cell lysates were subjected to SDS-PAGE, transferred onto a polyvinylidenedifluoride membrane (Roche, catalog 03010040001), followed by incubation overnight at 4 °C with primary antibodies. The membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 2 h. Proteins were then detected with the enhanced chemiluminescence methods.

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