Nucleofection with 1 μM si-IRE1α (4390824, ThermoFisher Scientific, Stockholm, Sweden), or 1 μM siCtrl (4390843, ThermoFisher Scientific, Stockholm, Sweden) was performed using Amaxa Nucleofector program T-028 (HepG2) in ice-cold Ingenio electroporation solution (MIR50114, Mirus Bio LLC, Taastrup, Denmark) on 1.0 × 106 cells per transfection. HepG2 cells were re-suspended in 2 mL DMEM with 10% FBS and left to adhere for 6 h, after which the medium was changed to fresh DMEM. Transfection efficiency was checked 24 h post-transfection by RT-PCR, achieving reduced mRNA expression by >60%.
Si ire1α
Manufactured by Thermo Fisher Scientific
Si-IRE1α is a laboratory equipment used for the detection and analysis of the endoplasmic reticulum stress sensor IRE1α. It provides a reliable and efficient method for researchers to study the cellular response to endoplasmic reticulum stress.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using si ire1α
1
Nucleofection of siRNA in HepG2 Cells
2
Nucleofection of si-IRE1α in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nucleofection with 0.1–1 µM si-IRE1α (s200432, ThermoFisher Scientific, Stockholm, Sweden), or 0.1 µM siCtrl (4390843, ThermoFisher Scientific, Stockholm, Sweden) was done using Amaxa Nucleofector program S-005 (LX2-cells) or T-028 (HepG2 and Huh7) in ice-cold Ingenio electroporation solution (MIR50114, Mirus Bio LLC, Taastrup, Denmark) on 1.0 × 106 cells per transfection. Cells were promptly re-suspended in 2 mL DMEM with 10% FBS and left adhere for 6–8 hr, after which the medium was changed to fresh DMEM. Transfection efficiency was checked 24 hr post-transfection by qPCR. Only one si-RNA was used, as this reduced mRNA expression by >40% in all cell lines.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!