Rabbit anti atp5a1

The fixed renal tissues were embedded in paraffin and made to 5 μm sections. Renal sections were deparaffinized in xylene and rehydrated in graded ethanols, and then stained with hematoxylin eosin (HE) and periodic acid-schiff (PAS) staining. For immunohistochemical (IHC) staining, sections were blocked with 1% BSA, and incubated with diluted primary antibodies including rabbit anti-α-SMA (Abcam, USA), mouse anti-MCP-1 (Abcam, USA), rabbit anti-IL-6 (Abcam, USA), rabbit anti-CPT1 (Proteintech, USA), rabbit anti-FABP1 (Proteintech, USA), rabbit anti-PPARα (Proteintech, USA), rabbit anti-UCP2 (Abcam, USA), rabbit anti-ATP5A1 (ABClonal, USA), rabbit anti-Sirt1 (CST, USA), rabbit anti-p-AMPK (CST, USA) and rabbit anti-PGC1α (Proteintech, USA), then incubated with HRP-conjugated secondary antibody (DAKO, USA), and finally stained with DAB substrate and hematoxylin. The images of stained sections were acquired by microscope (Carl Zeiss, Germany), and quantitative analysis of positive staining areas (%) in images was done by using Image J software (NIH, USA).Five images of non-overlapping fields were captured from each section per animal, and the pathological score of renal injury including glomerulosclerosis, interstitial fibrosis and glomerular size were performed as previously described [20 (link), 21 (link)].