G007 lk

Manufactured by Selleck Chemicals

Sourced in United States

The G007-LK is a laboratory equipment designed for general use in chemical and scientific research applications. It serves as a versatile tool for various tasks within a laboratory setting. The core function of the G007-LK is to provide a stable and controlled environment for conducting experiments and analyses.

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Lab products found in correlation

8 protocols using g007 lk

Comprehensive Small Molecule Evaluation

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AGI-5198 was provided by Agios. Debio1347 was obtained from DebioPharm. G007-LK and LGK-974 were purchased from Selleckchem.

Chatila W.K., Walch H., Hechtman J.F., Moyer S.M., Sgambati V., Faleck D.M., Srivastava A., Tang L., Benhamida J., Ismailgeci D., Campos C., Wu F., Chang Q., Vakiani E., de Stanchina E., Weiser M.R., Widmar M., Yantiss R.K., Shah M.A., Bass A.J., Stadler Z.K., Katz L.H., Mellinghoff I.K., Sethi N.S., Schultz N., Ganesh K., Kelsen D, & Yaeger R. (2023). Integrated clinical and genomic analysis identifies driver events and molecular evolution of colitis-associated cancers. Nature Communications, 14, 110.

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Regulatory Signaling Pathways in Osteoclastogenesis

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Recombinant murine and human M-CSF and RANKL proteins were purchased from Peprotech (Rocky Hill, NJ, USA). IWR-1-endo (IWR-1) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). G007-LK, XAV939, and ICG-001 were purchased from Selleck Chemicals (Houston, TX, USA). FK506 was obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-SH3BP2 antibody (H00006452-M01) was obtained from Abcam (Cambridge, MA, USA). Anti-ACTIN antibody (A2066) was obtained from Sigma-Aldrich. Anti-Phospho-SYK (Tyr352) antibody (2701), anti-total SYK antibody (13198), anti-NUP98 antibody (2598), anti-GAPDH antibody (2118), anti-c-FOS antibody (2250), anti-AXIN1 antibody (2074), anti-β-catenin antibody (8480), anti-RUNX2 antibody (8486), HRP-conjugated anti-rabbit IgG antibody (7074), and HRP-conjugated anti-mouse IgG antibody (7076) were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-NFATc1 antibody (sc-7294), anti-NF-κB p50 antibody (sc-1190), anti-ABL antibody (sc-56887), and HRP-conjugated anti-goat IgG antibody (sc-2354) were obtained from Santa Cruz Biotechnology.

Fujita S., Mukai T., Mito T., Kodama S., Nagasu A., Kittaka M., Sone T., Ueki Y, & Morita Y. (2017). Pharmacological inhibition of tankyrase induces bone loss in mice by increasing osteoclastogenesis. Bone, 106, 156-166.

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Optimized Storage and Handling of Cell Culture Drugs

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XAV939, NVP636, G007-LK, Palbociclib, Gefitinib and Trametinib were purchased form Selleckchem. All drugs were aliquoted at 20mM stocks and stored at -80C indefinitely. 1mM aliquots were prepared from these stocks and stored at -20C for 6–12 months. 1mM aliquots were thawed once and aliquots in use were kept at 4C for a period <2 weeks (leftovers were discarded if left unused after these periods of time). The working concentrations are indicated in each Fig panel. Drugs in solution for cellular treatment were replenished every 48h by adding fresh media.

Foronda M., Tarumoto Y., Schatoff E.M., Leach B.I., Diaz B.J., Zimmerman J., Goswami S., Shusterman M., Vakoc C.R, & Dow L.E. (2019). Tankyrase inhibition sensitizes cells to CDK4 blockade. PLoS ONE, 14(12), e0226645.

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Cell Viability Assay with THP-1 Cells

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THP-1 cell lines were cultured in RPMI supplemented with 10% FCS, 100 μg ml⁻¹ of penicillin and 100 μg ml⁻¹ of streptomycin and 1 μg ml⁻¹ puromycin. ABT-199 was purchased from ChemieTek (Indianopolis, IN). Cantharidin, digoxigenin, proscardillin, wortmannin, SB203580, TOFA, IC 261 and vorinostat were all purchased from Sigma. ABT-737, GSK-J4, GSK-126, G007-LK, MM-102, SKI-606 and JNK-IN-8 were purchased from Sellekchem (Houston, TX). LB100 (HY-18597) was obtained from MedChemExpress (MonMouth Junction, NJ). After 4 days of Dox induction (or control without Dox), cells were plated in RPMI 20% foetal bovine serum at 2 × 10⁵ ml⁻¹ in 96-well plate with twofold dilutions of each drug performed in duplicate. At 72 h, cell viability was measured using a plate-reader after addition of 10% Presto Blue Cell Viability Reagent (ThermoFisher Scientific) at emission fluorescence 590 nm. IC₅₀ curves were calculated for each drug in the presence and absence of Dox using GraphPad Prism 6.0 (dose response inhibition) and the difference in IC₅₀ was plotted as a percentage of control (no dox). For ACACA validation, transduced THP-1 cells were induced with Dox for 3 days, then washed into low serum RPMI (0.5%) and cultured at low cell density for 7 days +/−TOFA before cell growth was measured with Presto Blue on a plate reader.

Sinha S., Thomas D., Chan S., Gao Y., Brunen D., Torabi D., Reinisch A., Hernandez D., Chan A., Rankin E.B., Bernards R., Majeti R, & Dill D.L. (2017). Systematic discovery of mutation-specific synthetic lethals by mining pan-cancer human primary tumor data. Nature Communications, 8, 15580.

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Modulating Wnt Signaling in Cells

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To stimulate or inhibit WNT signaling activity, cells were treated for 24 h with Wnt3A CM or G007-LK, respectively. Wnt3A CM was prepared by collecting the supernatant from L Wnt3A cells (ATCC CRL-2647) according to the manufactures protocol. Unless otherwise stated, HEK293 cells were treated with Wnt3A CM diluted 50% in complete DMEM. Stock solutions of G007-LK [16 (link)] (and Selleck Chemicals, Houston, TX, USA) was prepared in DMSO and was added to the growth medium at a final concentration of 1 µm.

Olsen P.A, & Krauss S. (2021). The Adenoviral E1B-55k Protein Present in HEK293 Cells Mediates Abnormal Accumulation of Key WNT Signaling Proteins in Large Cytoplasmic Aggregates. Genes, 12(12), 1920.

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PARP Inhibitor Compounds Evaluation

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The PARP inhibitors used in this study were purchased from Selleckchem (Houston, TX, USA) with the following names and catalog numbers: A-966492 (catalog number S2197), AG14361 (catalog number S2178), AZD2461 (catalog number S7029), E7449 (catalog number S8419), G007-LK (catalog number S7239), Niraparib (MK-4827; catalog number S2741), NMS-P118 (catalog number S8363), NU1025 (catalog number S7730), Olaparib (AZD2281, Ku-0059436; catalog number S1060), PJ34-HCl (catalog number S7300), Rucaparib (AG-014699, PF-01367338; catalog number S1098), Talazoparib (BMN673; catalog number S7048), and UPF1069 (catalog number S8038). Stock solutions were dissolved in dimethyl sulfoxide (DMSO) according to the manufacturer’s protocol to a concentration of 10 mM and stored at −20 °C. Table 1 lists the PARP inhibitors used in this study and their proposed targets.

Keung M.Y., Wu Y., Badar F, & Vadgama J.V. (2020). Response of Breast Cancer Cells to PARP Inhibitors Is Independent of BRCA Status. Journal of Clinical Medicine, 9(4), 940.

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Tankyrase Inhibitors Sensitize Cancer Cells

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A549 cells seeded in 6-well plates (500 cells/well) were treated with tankyrase inhibitors [XAV939 (#3748, Tocris Bioscience, Bristol, United Kingdom), JW55 (#4514, Tocris Bioscience), TNKS656 (provided by Dr. Fumiyuki Shirai, RIKEN) and G007-LK (S7239, Selleck Chemicals, Houston, TX) or a PARP1/2 inhibitor (olaparib, S1060, Selleck Chemicals)] at 3 μM for 16 h, treated with X-ray irradiation at various doses and then cultured for 10 days. In another assay, cells pretreated with or without 3 μM XAV939, 3 μM G007-LK or 3 μM olaparib for 16 h were treated with various concentrations of DNA-damaging anticancer drugs [bleomycin (BML-AP302, Enzo Biochem, Farmingdale, NY), doxorubicin (D1515, Sigma-Aldrich), etoposide (BML-GR307, Enzo Biochem), cisplatin (ALX-400-40, Enzo Biochem) or camptothecin] in the presence or absence of 3 μM XAV939 and cultured for 10 days. Colonies were stained with 0.5% crystal violet/25% methanol and quantified by colony analyzer CA-7II (System Science Co., Tokyo, Japan) or ImageJ software (National Institute of Health, Bethesda, MD).

Okamoto K., Ohishi T., Kuroiwa M., Iemura S.I., Natsume T, & Seimiya H. (2018). MERIT40-dependent recruitment of tankyrase to damaged DNA and its implication for cell sensitivity to DNA-damaging anticancer drugs. Oncotarget, 9(88), 35844-35855.

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Prostate Cancer Cell Line Generation

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LNCaP and Myc-CAP cells were provided by P.A. Watson. Primary murine fibroblasts from C57BL/6 mice were purchased from Cell Biologics, Inc and grown in complete fibroblast media (M2267). MPt, MP, and MPApc murine prostate cancer cell lines were derived from EPO-GEMM prostate tumors with these genotypes. To generate these cell lines, prostate tumors were minced, digested in DMEM media containing 3 mg/ml Dispase II (Gibco) and 1 mg/ml Collagenase IV (C5138;Sigma) for 1 hour at 37^oC, and plated on 10 cm culture dishes coated with 100 μg/ml collagen (PureCol) (5005; Advanced Biomatrix). Primary cultures were passaged at least 3 times to remove fibroblast contamination. All prostate cancer cell lines were maintained in a humidified incubator at 37^oC with 5% CO₂, and grown in RPMI 1640 or DMEM supplemented with 10% FBS and 100 IU ml⁻¹ penicillin/streptomycin. All cell lines used were negative for mycoplasma.
Enzalutamide (S1250) and G007-LK (S7239) were all purchased from Selleck chemicals for in vitro studies. Drugs for in vitro studies were dissolved in DMSO (vehicle) to yield 10 mM stock solutions and stored at −80°C. For in vitro studies, growth media with or without drugs was changed every 3 days. For in vivo studies, G007-LK (B5830) was purchased from APExBIO. G007-LK was dissolved in 10% DMSO and then reconstitued in 20% Cremophor EL (Sigma-Aldrich) in saline.

Leibold J., Ruscetti M., Cao Z., Ho Y.J., Baslan T., Zou M., Abida W., Feucht J., Han T., Barriga F.M., Tsanov K.M., Zamechek L., Kulick A., Amor C., Tian S., Rybczyk K., Salgado N.R., Sánchez-Rivera F.J., Watson P.A., de Stanchina E., Wilkinson J.E., Dow L.E., Abate-Shen C., Sawyers C.L, & Lowe S.W. (2020). Somatic tissue engineering in mouse models reveals an actionable role for WNT pathway alterations in prostate cancer metastasis. Cancer discovery, 10(7), 1038-1057.

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