For digestion of the extracellular domain of human RAGE (soluble RAGE, or sRAGE), 5 μg sRAGE was digested by 20 ng mCtsK in CtsK digestion buffer at 22 °C in the presence of absence of 12mer for up to 20 mins.
Z phe arg pna
Z-Phe-Arg-pNA is a laboratory reagent used in biochemical and analytical applications. It functions as a chromogenic substrate for the detection and quantification of proteolytic enzymes, particularly those with arginine-specific activity.
2 protocols using z phe arg pna
Cathepsin K Activity Assays
For digestion of the extracellular domain of human RAGE (soluble RAGE, or sRAGE), 5 μg sRAGE was digested by 20 ng mCtsK in CtsK digestion buffer at 22 °C in the presence of absence of 12mer for up to 20 mins.
Substrate Specificity Analysis of Proteases
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